Sperm enzyme activity analysis in individual sperm for detection of heritable mutations in mammals
Male mice were injected i.p. with 2.5 mg/kg mitomycin C, 100 mg/kg ethyl nitrosourea or saline and mated with untreated virgin females five weeks later. Sperm from 64 of the F1 male progeny were analyzed histochemically for acrosin, succinic dehydrogenase and alpha-glycerophosphate dehydrogenase act...
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| Veröffentlicht in: | Experientia 1988-03, Vol.44 (3), p.253-254 |
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| container_title | Experientia |
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| creator | Panda, B.B Ficsor, G Ginsberg, L.C |
| description | Male mice were injected i.p. with 2.5 mg/kg mitomycin C, 100 mg/kg ethyl nitrosourea or saline and mated with untreated virgin females five weeks later. Sperm from 64 of the F1 male progeny were analyzed histochemically for acrosin, succinic dehydrogenase and alpha-glycerophosphate dehydrogenase activity. The frequency of F1 males with sub-normal sperm enzyme activity was significantly higher among progeny from treated males than in controls. These results show that analysis of sperm enzyme activity in F1 males is a practical method for detection of transmitted mutations induced in a treated parent. |
| doi_str_mv | 10.1007/BF01941727 |
| format | Article |
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Sperm from 64 of the F1 male progeny were analyzed histochemically for acrosin, succinic dehydrogenase and alpha-glycerophosphate dehydrogenase activity. The frequency of F1 males with sub-normal sperm enzyme activity was significantly higher among progeny from treated males than in controls. These results show that analysis of sperm enzyme activity in F1 males is a practical method for detection of transmitted mutations induced in a treated parent.</description><identifier>ISSN: 0014-4754</identifier><identifier>EISSN: 1420-9071</identifier><identifier>DOI: 10.1007/BF01941727</identifier><identifier>PMID: 3127237</identifier><identifier>CODEN: EXPEAM</identifier><language>eng</language><publisher>Basel: Birkhäuser</publisher><subject>Acrosin - metabolism ; Animals ; Biological and medical sciences ; enzyme activity ; Ethylnitrosourea - pharmacology ; Fundamental and applied biological sciences. Psychology ; Glycerolphosphate Dehydrogenase - metabolism ; heritability ; Male ; Mammalian male genital system ; mammals ; Mice ; Mitomycin ; Mitomycins - pharmacology ; Morphology. Physiology ; Mutagenicity Tests ; mutation ; spermatozoa ; Spermatozoa - enzymology ; Succinate Dehydrogenase - metabolism ; Vertebrates: reproduction</subject><ispartof>Experientia, 1988-03, Vol.44 (3), p.253-254</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c325t-5a16c25582d3751d1e284e9f263881c528fe76ed71d7ed85e2b0c12427eda4f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7103176$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3127237$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Panda, B.B</creatorcontrib><creatorcontrib>Ficsor, G</creatorcontrib><creatorcontrib>Ginsberg, L.C</creatorcontrib><title>Sperm enzyme activity analysis in individual sperm for detection of heritable mutations in mammals</title><title>Experientia</title><addtitle>Experientia</addtitle><description>Male mice were injected i.p. with 2.5 mg/kg mitomycin C, 100 mg/kg ethyl nitrosourea or saline and mated with untreated virgin females five weeks later. Sperm from 64 of the F1 male progeny were analyzed histochemically for acrosin, succinic dehydrogenase and alpha-glycerophosphate dehydrogenase activity. The frequency of F1 males with sub-normal sperm enzyme activity was significantly higher among progeny from treated males than in controls. These results show that analysis of sperm enzyme activity in F1 males is a practical method for detection of transmitted mutations induced in a treated parent.</description><subject>Acrosin - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>enzyme activity</subject><subject>Ethylnitrosourea - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerolphosphate Dehydrogenase - metabolism</subject><subject>heritability</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>mammals</subject><subject>Mice</subject><subject>Mitomycin</subject><subject>Mitomycins - pharmacology</subject><subject>Morphology. Physiology</subject><subject>Mutagenicity Tests</subject><subject>mutation</subject><subject>spermatozoa</subject><subject>Spermatozoa - enzymology</subject><subject>Succinate Dehydrogenase - metabolism</subject><subject>Vertebrates: reproduction</subject><issn>0014-4754</issn><issn>1420-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M9LHTEQB_AgFfuqXryX5lB6KKzO5Mdm92ilVkHwoJ6XvGTSbtnsvia7hedf3-h7WAgMmfnMHL6MnSGcI4C5-HYN2Co0whywFSoBVQsG37EVAKpKGa3esw85_waQoLE-YkcShRHSrNj6YUMpchqft5G4dXP_t5-33I522OY-834sz5emX-zA8ysOU-KeZip4GvkU-C9K_WzXA_G4zPal-7oYbYx2yCfsMJRCp_t6zJ6uvz9e3VR39z9ury7vKieFnittsXZC60Z4aTR6JNEoaoOoZdOg06IJZGryBr0h32gSa3AolCg_q4KQx-zL7u4mTX8WynMX--xoGOxI05I7VC20DaoCv-6gS1POiUK3SX20adshdC-Bdv8DLfjj_uqyjuTf6D7BMv-8n9vs7BCSHV2f35hBkGjqwj7tWLBTZ3-mQp4eBKAEAUZpaOQ_eGeGbA</recordid><startdate>19880315</startdate><enddate>19880315</enddate><creator>Panda, B.B</creator><creator>Ficsor, G</creator><creator>Ginsberg, L.C</creator><general>Birkhäuser</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19880315</creationdate><title>Sperm enzyme activity analysis in individual sperm for detection of heritable mutations in mammals</title><author>Panda, B.B ; Ficsor, G ; Ginsberg, L.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-5a16c25582d3751d1e284e9f263881c528fe76ed71d7ed85e2b0c12427eda4f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Acrosin - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>enzyme activity</topic><topic>Ethylnitrosourea - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycerolphosphate Dehydrogenase - metabolism</topic><topic>heritability</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>mammals</topic><topic>Mice</topic><topic>Mitomycin</topic><topic>Mitomycins - pharmacology</topic><topic>Morphology. Physiology</topic><topic>Mutagenicity Tests</topic><topic>mutation</topic><topic>spermatozoa</topic><topic>Spermatozoa - enzymology</topic><topic>Succinate Dehydrogenase - metabolism</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panda, B.B</creatorcontrib><creatorcontrib>Ficsor, G</creatorcontrib><creatorcontrib>Ginsberg, L.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Experientia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panda, B.B</au><au>Ficsor, G</au><au>Ginsberg, L.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm enzyme activity analysis in individual sperm for detection of heritable mutations in mammals</atitle><jtitle>Experientia</jtitle><addtitle>Experientia</addtitle><date>1988-03-15</date><risdate>1988</risdate><volume>44</volume><issue>3</issue><spage>253</spage><epage>254</epage><pages>253-254</pages><issn>0014-4754</issn><eissn>1420-9071</eissn><coden>EXPEAM</coden><abstract>Male mice were injected i.p. with 2.5 mg/kg mitomycin C, 100 mg/kg ethyl nitrosourea or saline and mated with untreated virgin females five weeks later. Sperm from 64 of the F1 male progeny were analyzed histochemically for acrosin, succinic dehydrogenase and alpha-glycerophosphate dehydrogenase activity. The frequency of F1 males with sub-normal sperm enzyme activity was significantly higher among progeny from treated males than in controls. These results show that analysis of sperm enzyme activity in F1 males is a practical method for detection of transmitted mutations induced in a treated parent.</abstract><cop>Basel</cop><cop>Boston, MA</cop><cop>Berlin</cop><pub>Birkhäuser</pub><pmid>3127237</pmid><doi>10.1007/BF01941727</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Experientia, 1988-03, Vol.44 (3), p.253-254 |
| issn | 0014-4754 1420-9071 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Acrosin - metabolism Animals Biological and medical sciences enzyme activity Ethylnitrosourea - pharmacology Fundamental and applied biological sciences. Psychology Glycerolphosphate Dehydrogenase - metabolism heritability Male Mammalian male genital system mammals Mice Mitomycin Mitomycins - pharmacology Morphology. Physiology Mutagenicity Tests mutation spermatozoa Spermatozoa - enzymology Succinate Dehydrogenase - metabolism Vertebrates: reproduction |
| title | Sperm enzyme activity analysis in individual sperm for detection of heritable mutations in mammals |
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