Sites of action of D2O in intact and skinned crayfish muscle fibers
The effect on tension development of replacing 90% of the H2O of the bathing saline with D2O was studied on intact single fibers, and on skinned fibers before and after the latter were treated so as to eliminate Ca-accumulation by the sarcoplasmic reticulum (SR). Excitation-contraction coupling (ECC...
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| Veröffentlicht in: | The Journal of membrane biology 1975-12, Vol.24 (3-4), p.249-263 |
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| container_end_page | 263 |
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| container_issue | 3-4 |
| container_start_page | 249 |
| container_title | The Journal of membrane biology |
| container_volume | 24 |
| creator | Eastwood, A B Grundfest, H Brandt, P W Reuben, J P |
| description | The effect on tension development of replacing 90% of the H2O of the bathing saline with D2O was studied on intact single fibers, and on skinned fibers before and after the latter were treated so as to eliminate Ca-accumulation by the sarcoplasmic reticulum (SR). Excitation-contraction coupling (ECC) of intact fibers is not abolished, but is depressed by D2O so that higher depolarizations are required to elicit a given tension. The reduction in tension at a given level of depolarization is not due to inhibition of the contractile system. The latter showed an enhanced Ca sensitivity; that is, skinned fibers respond to Ca concentrations that are 1-2 orders of magnitude smaller in D2O than in H2O saline. When bathed in D2O saline, intact fibers or skinned fibers with functional SR can still accumulate and release Ca in sufficient quantities to allow repeated induction of maximum tensions. Relaxation is slowed in all three types of preparation, perhaps because of an increased affinity of troponin to Ca in D2O salines. |
| doi_str_mv | 10.1007/BF01868626 |
| format | Article |
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Excitation-contraction coupling (ECC) of intact fibers is not abolished, but is depressed by D2O so that higher depolarizations are required to elicit a given tension. The reduction in tension at a given level of depolarization is not due to inhibition of the contractile system. The latter showed an enhanced Ca sensitivity; that is, skinned fibers respond to Ca concentrations that are 1-2 orders of magnitude smaller in D2O than in H2O saline. When bathed in D2O saline, intact fibers or skinned fibers with functional SR can still accumulate and release Ca in sufficient quantities to allow repeated induction of maximum tensions. Relaxation is slowed in all three types of preparation, perhaps because of an increased affinity of troponin to Ca in D2O salines.</description><identifier>ISSN: 0022-2631</identifier><identifier>EISSN: 1432-1424</identifier><identifier>DOI: 10.1007/BF01868626</identifier><identifier>PMID: 814241</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Astacoidea ; Caffeine - pharmacology ; Calcium - metabolism ; Calcium - pharmacology ; Deuterium - pharmacology ; Egtazic Acid - pharmacology ; Glycine - analogs & derivatives ; Glycine - pharmacology ; Manganese - pharmacology ; Membrane Potentials ; Muscle Contraction - drug effects ; Muscles - drug effects ; Muscles - metabolism ; Potassium - pharmacology ; Potassium Chloride - pharmacology ; Sarcoplasmic Reticulum - metabolism ; Sarcoplasmic Reticulum - physiology ; Troponin - metabolism</subject><ispartof>The Journal of membrane biology, 1975-12, Vol.24 (3-4), p.249-263</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c250t-f936a72e6d74c321a12e565e03d3a127ed1754d0cefb15537c4c8bab4e841f413</citedby><cites>FETCH-LOGICAL-c250t-f936a72e6d74c321a12e565e03d3a127ed1754d0cefb15537c4c8bab4e841f413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/814241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eastwood, A B</creatorcontrib><creatorcontrib>Grundfest, H</creatorcontrib><creatorcontrib>Brandt, P W</creatorcontrib><creatorcontrib>Reuben, J P</creatorcontrib><title>Sites of action of D2O in intact and skinned crayfish muscle fibers</title><title>The Journal of membrane biology</title><addtitle>J Membr Biol</addtitle><description>The effect on tension development of replacing 90% of the H2O of the bathing saline with D2O was studied on intact single fibers, and on skinned fibers before and after the latter were treated so as to eliminate Ca-accumulation by the sarcoplasmic reticulum (SR). Excitation-contraction coupling (ECC) of intact fibers is not abolished, but is depressed by D2O so that higher depolarizations are required to elicit a given tension. The reduction in tension at a given level of depolarization is not due to inhibition of the contractile system. The latter showed an enhanced Ca sensitivity; that is, skinned fibers respond to Ca concentrations that are 1-2 orders of magnitude smaller in D2O than in H2O saline. When bathed in D2O saline, intact fibers or skinned fibers with functional SR can still accumulate and release Ca in sufficient quantities to allow repeated induction of maximum tensions. Relaxation is slowed in all three types of preparation, perhaps because of an increased affinity of troponin to Ca in D2O salines.</description><subject>Animals</subject><subject>Astacoidea</subject><subject>Caffeine - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Calcium - pharmacology</subject><subject>Deuterium - pharmacology</subject><subject>Egtazic Acid - pharmacology</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - pharmacology</subject><subject>Manganese - pharmacology</subject><subject>Membrane Potentials</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscles - drug effects</subject><subject>Muscles - metabolism</subject><subject>Potassium - pharmacology</subject><subject>Potassium Chloride - pharmacology</subject><subject>Sarcoplasmic Reticulum - metabolism</subject><subject>Sarcoplasmic Reticulum - physiology</subject><subject>Troponin - metabolism</subject><issn>0022-2631</issn><issn>1432-1424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1975</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1LxDAUxIP4VVcvnj3kLFTz8lmPWl0VFvagnkuavGB02y5N97D_vS0VhYE3DD8ezBByCewGGDO3D0sGhS401wckAyl4DpLLQ5IxxnnOtYBTcpbSF2NgjJYn5LiYAMhI-RYHTLQL1Lohdu3kHvmaxnbUMGbUtp6m79i26Knr7T7E9EmbXXIbpCHW2KdzchTsJuHF712Qj-XTe_mSr9bPr-X9KndcsSEPd0Jbw1F7I53gYIGj0gqZ8GL0Bj0YJT1zGGpQShgnXVHbWmIhIUgQC3I9_3V9l1KPodr2sbH9vgJWTTtU_zuM8NUMb3d1g_4PnYuLHynpVhk</recordid><startdate>197512</startdate><enddate>197512</enddate><creator>Eastwood, A B</creator><creator>Grundfest, H</creator><creator>Brandt, P W</creator><creator>Reuben, J P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>197512</creationdate><title>Sites of action of D2O in intact and skinned crayfish muscle fibers</title><author>Eastwood, A B ; Grundfest, H ; Brandt, P W ; Reuben, J P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c250t-f936a72e6d74c321a12e565e03d3a127ed1754d0cefb15537c4c8bab4e841f413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1975</creationdate><topic>Animals</topic><topic>Astacoidea</topic><topic>Caffeine - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Calcium - pharmacology</topic><topic>Deuterium - pharmacology</topic><topic>Egtazic Acid - pharmacology</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - pharmacology</topic><topic>Manganese - pharmacology</topic><topic>Membrane Potentials</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscles - drug effects</topic><topic>Muscles - metabolism</topic><topic>Potassium - pharmacology</topic><topic>Potassium Chloride - pharmacology</topic><topic>Sarcoplasmic Reticulum - metabolism</topic><topic>Sarcoplasmic Reticulum - physiology</topic><topic>Troponin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eastwood, A B</creatorcontrib><creatorcontrib>Grundfest, H</creatorcontrib><creatorcontrib>Brandt, P W</creatorcontrib><creatorcontrib>Reuben, J P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The Journal of membrane biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eastwood, A B</au><au>Grundfest, H</au><au>Brandt, P W</au><au>Reuben, J P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sites of action of D2O in intact and skinned crayfish muscle fibers</atitle><jtitle>The Journal of membrane biology</jtitle><addtitle>J Membr Biol</addtitle><date>1975-12</date><risdate>1975</risdate><volume>24</volume><issue>3-4</issue><spage>249</spage><epage>263</epage><pages>249-263</pages><issn>0022-2631</issn><eissn>1432-1424</eissn><abstract>The effect on tension development of replacing 90% of the H2O of the bathing saline with D2O was studied on intact single fibers, and on skinned fibers before and after the latter were treated so as to eliminate Ca-accumulation by the sarcoplasmic reticulum (SR). Excitation-contraction coupling (ECC) of intact fibers is not abolished, but is depressed by D2O so that higher depolarizations are required to elicit a given tension. The reduction in tension at a given level of depolarization is not due to inhibition of the contractile system. The latter showed an enhanced Ca sensitivity; that is, skinned fibers respond to Ca concentrations that are 1-2 orders of magnitude smaller in D2O than in H2O saline. When bathed in D2O saline, intact fibers or skinned fibers with functional SR can still accumulate and release Ca in sufficient quantities to allow repeated induction of maximum tensions. Relaxation is slowed in all three types of preparation, perhaps because of an increased affinity of troponin to Ca in D2O salines.</abstract><cop>United States</cop><pmid>814241</pmid><doi>10.1007/BF01868626</doi><tpages>15</tpages></addata></record> |
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| issn | 0022-2631 1432-1424 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Astacoidea Caffeine - pharmacology Calcium - metabolism Calcium - pharmacology Deuterium - pharmacology Egtazic Acid - pharmacology Glycine - analogs & derivatives Glycine - pharmacology Manganese - pharmacology Membrane Potentials Muscle Contraction - drug effects Muscles - drug effects Muscles - metabolism Potassium - pharmacology Potassium Chloride - pharmacology Sarcoplasmic Reticulum - metabolism Sarcoplasmic Reticulum - physiology Troponin - metabolism |
| title | Sites of action of D2O in intact and skinned crayfish muscle fibers |
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