The use of an alginic acid matrix to support in vitro development of isolated murine blastomeres

The present study reports the use of a cross-linked calcium alginate matrix to replace the zona pellucida in supporting in vitro development of isolated two-cell murine blastomeres. The zona pellucidae were enzymatically removed with a 0.5% pronase solution and blastomeres separated with a fine-bore...

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Veröffentlicht in:	Journal of In Vitro Fertilization and Embryo Transfer 1990-02, Vol.7 (1), p.28-32
Hauptverfasser:	EATON, N. L, NIEMEYER, G. P, DOODY, M. C
Format:	Artikel
Sprache:	eng
Schlagworte:	Alginates - pharmacology Animals Biological and medical sciences Blastomeres - physiology Culture Media Early stages. Segmentation. Gastrulation. Neurulation Embryo, Mammalian - cytology Embryo, Mammalian - physiology Embryology: invertebrates and vertebrates. Teratology Fundamental and applied biological sciences. Psychology Glucuronic Acid Hexuronic Acids In Vitro Techniques Mice Mice, Inbred Strains Zona Pellucida - physiology
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