Determination of lysozyme content in eggs and egg products using SDS-gel electrophoresis
Lysozyme can be separated commercially from other egg components and is used as a natural, antibiotically active protein. A method for the detection of de-lysozymed egg products using SDS-polyacrylamide gel electrophoresis (PAGE) is described. The method can also be used for lysozyme quantification....
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| Veröffentlicht in: | Zeitschrift f r Lebensmittel-Untersuchung und -Forschung 1988-12, Vol.187 (6), p.541-545 |
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| container_end_page | 545 |
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| container_issue | 6 |
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| container_title | Zeitschrift f r Lebensmittel-Untersuchung und -Forschung |
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| creator | WETH, F SCHROEDER, T BUXTORF, U. P |
| description | Lysozyme can be separated commercially from other egg components and is used as a natural, antibiotically active protein. A method for the detection of de-lysozymed egg products using SDS-polyacrylamide gel electrophoresis (PAGE) is described. The method can also be used for lysozyme quantification. Lysozyme concentrations in egg products are tentatively assigned to three classes, based on the lysozyme contents found in intact eggs. The results obtained from a series of egg products on the market allow two groups to be distinguished. The first group has values for average lysozyme content, standard deviation and total protein content very close to the values found for intact eggs, and the second group clearly shows a lower lysozyme content but a total protein content comparable with intact eggs. |
| doi_str_mv | 10.1007/BF01042386 |
| format | Article |
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The first group has values for average lysozyme content, standard deviation and total protein content very close to the values found for intact eggs, and the second group clearly shows a lower lysozyme content but a total protein content comparable with intact eggs.</description><identifier>ISSN: 0044-3026</identifier><identifier>EISSN: 1438-2385</identifier><identifier>DOI: 10.1007/BF01042386</identifier><identifier>PMID: 3223094</identifier><identifier>CODEN: ZLUFAR</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Biological and medical sciences ; Densitometry ; Egg and egg product industries ; Egg Proteins - analysis ; Egg White - analysis ; Eggs - analysis ; Electrophoresis, Polyacrylamide Gel ; Food industries ; Fundamental and applied biological sciences. 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The first group has values for average lysozyme content, standard deviation and total protein content very close to the values found for intact eggs, and the second group clearly shows a lower lysozyme content but a total protein content comparable with intact eggs.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Densitometry</subject><subject>Egg and egg product industries</subject><subject>Egg Proteins - analysis</subject><subject>Egg White - analysis</subject><subject>Eggs - analysis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. 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P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of lysozyme content in eggs and egg products using SDS-gel electrophoresis</atitle><jtitle>Zeitschrift f r Lebensmittel-Untersuchung und -Forschung</jtitle><addtitle>Z Lebensm Unters Forsch</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>187</volume><issue>6</issue><spage>541</spage><epage>545</epage><pages>541-545</pages><issn>0044-3026</issn><eissn>1438-2385</eissn><coden>ZLUFAR</coden><abstract>Lysozyme can be separated commercially from other egg components and is used as a natural, antibiotically active protein. A method for the detection of de-lysozymed egg products using SDS-polyacrylamide gel electrophoresis (PAGE) is described. The method can also be used for lysozyme quantification. Lysozyme concentrations in egg products are tentatively assigned to three classes, based on the lysozyme contents found in intact eggs. 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| ispartof | Zeitschrift f r Lebensmittel-Untersuchung und -Forschung, 1988-12, Vol.187 (6), p.541-545 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Biological and medical sciences Densitometry Egg and egg product industries Egg Proteins - analysis Egg White - analysis Eggs - analysis Electrophoresis, Polyacrylamide Gel Food industries Fundamental and applied biological sciences. Psychology Muramidase - analysis |
| title | Determination of lysozyme content in eggs and egg products using SDS-gel electrophoresis |
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