Picogram detection levels of asialofetuin via the carbohydrate moieties using the light addressable potentiometric sensor
Fetal calf serum asialofetuin was assayed in the sandwich format using biotinylated and fluoresceinated ricin toxin (B-RCA and F-RCA). The sandwiched species was captured on a biotin-BSA coated nitrocellulose membrane with streptavidin. Anti-fluorescein antibody-urease conjugate was bound to the com...
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| Veröffentlicht in: | Glycoconjugate journal 1995-10, Vol.12 (5), p.660-663 |
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| container_title | Glycoconjugate journal |
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| creator | Dill, K Olson, J D |
| description | Fetal calf serum asialofetuin was assayed in the sandwich format using biotinylated and fluoresceinated ricin toxin (B-RCA and F-RCA). The sandwiched species was captured on a biotin-BSA coated nitrocellulose membrane with streptavidin. Anti-fluorescein antibody-urease conjugate was bound to the complex, and detected and quantitated under microvolume conditions using the light addressable potentiometric sensor. As little as 250 pg of asialofetuin was detectable whereas fetuin gave no response at conditions as high as 32 ng. Using a competitive inhibition assay, we established that the binding constant for the asialofetuin-ricin complex was 3.6 x 10(8) M-1. This is in good agreement with data published using glycopeptides derived from asialofetuin, and RCA and the ricin agglutinin, RCA-120. |
| doi_str_mv | 10.1007/BF00731262 |
| format | Article |
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This is in good agreement with data published using glycopeptides derived from asialofetuin, and RCA and the ricin agglutinin, RCA-120.</description><identifier>ISSN: 0282-0080</identifier><identifier>EISSN: 1573-4986</identifier><identifier>DOI: 10.1007/BF00731262</identifier><identifier>PMID: 8595257</identifier><language>eng</language><publisher>United States</publisher><subject>alpha-Fetoproteins - analysis ; alpha-Fetoproteins - chemistry ; Animals ; Asialoglycoproteins - analysis ; Asialoglycoproteins - chemistry ; Biotin ; Carbohydrate Conformation ; Carbohydrate Sequence ; Cattle ; Fetuins ; Fluoresceins ; Light ; Microchemistry - methods ; Molecular Sequence Data ; Oligosaccharides - chemistry ; Oligosaccharides - isolation & purification ; Potentiometry - methods ; Ricin ; Serine ; Threonine</subject><ispartof>Glycoconjugate journal, 1995-10, Vol.12 (5), p.660-663</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-6074cca952ccd76debcef40e18927e109cba245201213c9f6cb3b7403fff4ab33</citedby><cites>FETCH-LOGICAL-c282t-6074cca952ccd76debcef40e18927e109cba245201213c9f6cb3b7403fff4ab33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8595257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dill, K</creatorcontrib><creatorcontrib>Olson, J D</creatorcontrib><title>Picogram detection levels of asialofetuin via the carbohydrate moieties using the light addressable potentiometric sensor</title><title>Glycoconjugate journal</title><addtitle>Glycoconj J</addtitle><description>Fetal calf serum asialofetuin was assayed in the sandwich format using biotinylated and fluoresceinated ricin toxin (B-RCA and F-RCA). 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This is in good agreement with data published using glycopeptides derived from asialofetuin, and RCA and the ricin agglutinin, RCA-120.</description><subject>alpha-Fetoproteins - analysis</subject><subject>alpha-Fetoproteins - chemistry</subject><subject>Animals</subject><subject>Asialoglycoproteins - analysis</subject><subject>Asialoglycoproteins - chemistry</subject><subject>Biotin</subject><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Cattle</subject><subject>Fetuins</subject><subject>Fluoresceins</subject><subject>Light</subject><subject>Microchemistry - methods</subject><subject>Molecular Sequence Data</subject><subject>Oligosaccharides - chemistry</subject><subject>Oligosaccharides - isolation & purification</subject><subject>Potentiometry - methods</subject><subject>Ricin</subject><subject>Serine</subject><subject>Threonine</subject><issn>0282-0080</issn><issn>1573-4986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEUhYMotVY37oWshdE85rnUYlUo6ELXQ3Lnpo3MTEqSFvrvjbbo5p7F_Th8HEKuObvjjFX3j4t0JRelOCFTXlQyy5u6PCVTJmqRMVazc3IRwhdLWC7qCZnURVOIopqS_bsFt_JqoB1GhGjdSHvcYR-oM1QFq3pnMG7tSHdW0bhGCsprt953XkWkg7MYLQa6DXZc_f57u1pHqrrOYwhK90g3LuKYqgeM3gINOAbnL8mZUX3Aq2POyOfi6WP-ki3fnl_nD8sMknzMyqQMoJIuQFeVHWpAkzPkdSMq5KwBrUReCMYFl9CYErTUVc6kMSZXWsoZuT30gncheDTtxttB-X3LWfszX_s_X4JvDvBmqwfs_tDjXvIbgXttrw</recordid><startdate>199510</startdate><enddate>199510</enddate><creator>Dill, K</creator><creator>Olson, J D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199510</creationdate><title>Picogram detection levels of asialofetuin via the carbohydrate moieties using the light addressable potentiometric sensor</title><author>Dill, K ; Olson, J D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-6074cca952ccd76debcef40e18927e109cba245201213c9f6cb3b7403fff4ab33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>alpha-Fetoproteins - analysis</topic><topic>alpha-Fetoproteins - chemistry</topic><topic>Animals</topic><topic>Asialoglycoproteins - analysis</topic><topic>Asialoglycoproteins - chemistry</topic><topic>Biotin</topic><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Cattle</topic><topic>Fetuins</topic><topic>Fluoresceins</topic><topic>Light</topic><topic>Microchemistry - methods</topic><topic>Molecular Sequence Data</topic><topic>Oligosaccharides - chemistry</topic><topic>Oligosaccharides - isolation & purification</topic><topic>Potentiometry - methods</topic><topic>Ricin</topic><topic>Serine</topic><topic>Threonine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dill, K</creatorcontrib><creatorcontrib>Olson, J D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Glycoconjugate journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dill, K</au><au>Olson, J D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Picogram detection levels of asialofetuin via the carbohydrate moieties using the light addressable potentiometric sensor</atitle><jtitle>Glycoconjugate journal</jtitle><addtitle>Glycoconj J</addtitle><date>1995-10</date><risdate>1995</risdate><volume>12</volume><issue>5</issue><spage>660</spage><epage>663</epage><pages>660-663</pages><issn>0282-0080</issn><eissn>1573-4986</eissn><abstract>Fetal calf serum asialofetuin was assayed in the sandwich format using biotinylated and fluoresceinated ricin toxin (B-RCA and F-RCA). The sandwiched species was captured on a biotin-BSA coated nitrocellulose membrane with streptavidin. Anti-fluorescein antibody-urease conjugate was bound to the complex, and detected and quantitated under microvolume conditions using the light addressable potentiometric sensor. As little as 250 pg of asialofetuin was detectable whereas fetuin gave no response at conditions as high as 32 ng. Using a competitive inhibition assay, we established that the binding constant for the asialofetuin-ricin complex was 3.6 x 10(8) M-1. This is in good agreement with data published using glycopeptides derived from asialofetuin, and RCA and the ricin agglutinin, RCA-120.</abstract><cop>United States</cop><pmid>8595257</pmid><doi>10.1007/BF00731262</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0282-0080 |
| ispartof | Glycoconjugate journal, 1995-10, Vol.12 (5), p.660-663 |
| issn | 0282-0080 1573-4986 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | alpha-Fetoproteins - analysis alpha-Fetoproteins - chemistry Animals Asialoglycoproteins - analysis Asialoglycoproteins - chemistry Biotin Carbohydrate Conformation Carbohydrate Sequence Cattle Fetuins Fluoresceins Light Microchemistry - methods Molecular Sequence Data Oligosaccharides - chemistry Oligosaccharides - isolation & purification Potentiometry - methods Ricin Serine Threonine |
| title | Picogram detection levels of asialofetuin via the carbohydrate moieties using the light addressable potentiometric sensor |
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