Light and electron microscopic localization of the N-terminal fragment of human pro-opiomelanocortin in the human pituitary gland and in neoplasms
Immunohistochemical localization of the N-terminal fragment (1-76) (NTF) of human pro-opiomelanocortin (POMC) was studied in human adult and fetal pituitary glands, as well as in pituitary adenomas associated with Cushing's syndrome and in ectopic ACTH-producing tumors. Comparison of localizati...
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| Veröffentlicht in: | Virchows Archiv A Pathological Anatomy and Histopathology 1985, Vol.408 (2-3), p.281-287 |
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| container_title | Virchows Archiv A Pathological Anatomy and Histopathology |
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| creator | OSAMURA, R. Y WATANABE, K SEIDAH, N. G CHAN, J. S. D CHRETIEN, M |
| description | Immunohistochemical localization of the N-terminal fragment (1-76) (NTF) of human pro-opiomelanocortin (POMC) was studied in human adult and fetal pituitary glands, as well as in pituitary adenomas associated with Cushing's syndrome and in ectopic ACTH-producing tumors. Comparison of localization between NTF and ACTH was performed using mirror sections. Our results indicated concomitant localization of NTF and ACTH in the same cells, not only in normal adult and fetal pituitaries but also in pituitary adenomas and ectopic ACTH producing tumours. Specificity of the NTF staining was confirmed by immunoabsorption. Negative staining of the bovine pituitary gland indicated the immunohistochemical localization of N-terminal (1-45) of human POMC as there is a known species difference in the sequence 1-45 between human and the bovine N-terminal fragment. Presence of NTF in cisterna of rough endoplasmic reticulum indicates its production by small cell carcinoma. These findings, together with the previous studies, suggest that the complete form of POMC is produced in the tumours as well as in normal pituitaries. |
| doi_str_mv | 10.1007/BF00707990 |
| format | Article |
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Y ; WATANABE, K ; SEIDAH, N. G ; CHAN, J. S. D ; CHRETIEN, M</creator><creatorcontrib>OSAMURA, R. Y ; WATANABE, K ; SEIDAH, N. G ; CHAN, J. S. D ; CHRETIEN, M</creatorcontrib><description>Immunohistochemical localization of the N-terminal fragment (1-76) (NTF) of human pro-opiomelanocortin (POMC) was studied in human adult and fetal pituitary glands, as well as in pituitary adenomas associated with Cushing's syndrome and in ectopic ACTH-producing tumors. Comparison of localization between NTF and ACTH was performed using mirror sections. Our results indicated concomitant localization of NTF and ACTH in the same cells, not only in normal adult and fetal pituitaries but also in pituitary adenomas and ectopic ACTH producing tumours. Specificity of the NTF staining was confirmed by immunoabsorption. Negative staining of the bovine pituitary gland indicated the immunohistochemical localization of N-terminal (1-45) of human POMC as there is a known species difference in the sequence 1-45 between human and the bovine N-terminal fragment. Presence of NTF in cisterna of rough endoplasmic reticulum indicates its production by small cell carcinoma. These findings, together with the previous studies, suggest that the complete form of POMC is produced in the tumours as well as in normal pituitaries.</description><identifier>ISSN: 0174-7398</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/BF00707990</identifier><identifier>PMID: 3002013</identifier><identifier>CODEN: VAAHDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Adenoma - metabolism ; Adenoma - ultrastructure ; Adult ; Biological and medical sciences ; Carcinoma, Small Cell - analysis ; Carcinoma, Small Cell - ultrastructure ; Fundamental and applied biological sciences. Psychology ; Histocytochemistry ; Humans ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Immunochemistry ; Infant ; Lung Neoplasms - analysis ; Lung Neoplasms - ultrastructure ; Microscopy, Electron ; Morphology. 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Y</creatorcontrib><creatorcontrib>WATANABE, K</creatorcontrib><creatorcontrib>SEIDAH, N. G</creatorcontrib><creatorcontrib>CHAN, J. S. D</creatorcontrib><creatorcontrib>CHRETIEN, M</creatorcontrib><title>Light and electron microscopic localization of the N-terminal fragment of human pro-opiomelanocortin in the human pituitary gland and in neoplasms</title><title>Virchows Archiv A Pathological Anatomy and Histopathology</title><addtitle>Virchows Arch A Pathol Anat Histopathol</addtitle><description>Immunohistochemical localization of the N-terminal fragment (1-76) (NTF) of human pro-opiomelanocortin (POMC) was studied in human adult and fetal pituitary glands, as well as in pituitary adenomas associated with Cushing's syndrome and in ectopic ACTH-producing tumors. Comparison of localization between NTF and ACTH was performed using mirror sections. Our results indicated concomitant localization of NTF and ACTH in the same cells, not only in normal adult and fetal pituitaries but also in pituitary adenomas and ectopic ACTH producing tumours. Specificity of the NTF staining was confirmed by immunoabsorption. Negative staining of the bovine pituitary gland indicated the immunohistochemical localization of N-terminal (1-45) of human POMC as there is a known species difference in the sequence 1-45 between human and the bovine N-terminal fragment. Presence of NTF in cisterna of rough endoplasmic reticulum indicates its production by small cell carcinoma. These findings, together with the previous studies, suggest that the complete form of POMC is produced in the tumours as well as in normal pituitaries.</description><subject>Adenoma - metabolism</subject><subject>Adenoma - ultrastructure</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Small Cell - analysis</subject><subject>Carcinoma, Small Cell - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histocytochemistry</subject><subject>Humans</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Immunochemistry</subject><subject>Infant</subject><subject>Lung Neoplasms - analysis</subject><subject>Lung Neoplasms - ultrastructure</subject><subject>Microscopy, Electron</subject><subject>Morphology. Functional localizations</subject><subject>Peptide Fragments - analysis</subject><subject>Pituitary Gland - analysis</subject><subject>Pituitary Neoplasms - metabolism</subject><subject>Pituitary Neoplasms - ultrastructure</subject><subject>Pro-Opiomelanocortin - analysis</subject><subject>Vertebrates: endocrinology</subject><issn>0174-7398</issn><issn>1432-2307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1v1DAQhi0EapfChXslH1APSIGxHSfOsa0oIK3gUs6R49i7Rv7Y2s4BfkZ_cR01KpLHPrzPjGfeQegDgc8EoP9yc1dv6IcBXqEdaRltKIP-NdoB6dumZ4M4R29z_gPAORH8DJ0xAAqE7dDj3h6OBcswY-20KikG7K1KMat4sgq7qKSz_2SxVYgGl6PGP5uik7dBOmySPHgdyiodFy8DPqXY1MzotZMhqpiKDbieNXEjbFlskekvPrj13zUqEHQ8OZl9fofeGOmyfr-9F-j33df72-_N_te3H7fX-0ZR2pVmaskAetBMdrOQRCraDVIoMw2czkQZbQSZVcumWXYtEDkrJaaOk9lQpajp2AW6eq5bW35YdC6jt1lpV5vScclj33HOey4q-OkZXG3JSZvxlKyvA4wExnUB4_8FVPhyq7pMXs8v6OZ41T9uuszV2mpgUDa_YEJASwmwJ5ZXkPE</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>OSAMURA, R. 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D ; CHRETIEN, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c226t-b4190e9e3a6d8a1ac269a8cfb952d1cfef81dc43bda6401adcc8b651df2cc2f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Adenoma - metabolism</topic><topic>Adenoma - ultrastructure</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Small Cell - analysis</topic><topic>Carcinoma, Small Cell - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histocytochemistry</topic><topic>Humans</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Immunochemistry</topic><topic>Infant</topic><topic>Lung Neoplasms - analysis</topic><topic>Lung Neoplasms - ultrastructure</topic><topic>Microscopy, Electron</topic><topic>Morphology. Functional localizations</topic><topic>Peptide Fragments - analysis</topic><topic>Pituitary Gland - analysis</topic><topic>Pituitary Neoplasms - metabolism</topic><topic>Pituitary Neoplasms - ultrastructure</topic><topic>Pro-Opiomelanocortin - analysis</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OSAMURA, R. Y</creatorcontrib><creatorcontrib>WATANABE, K</creatorcontrib><creatorcontrib>SEIDAH, N. G</creatorcontrib><creatorcontrib>CHAN, J. S. D</creatorcontrib><creatorcontrib>CHRETIEN, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virchows Archiv A Pathological Anatomy and Histopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OSAMURA, R. Y</au><au>WATANABE, K</au><au>SEIDAH, N. G</au><au>CHAN, J. S. D</au><au>CHRETIEN, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Light and electron microscopic localization of the N-terminal fragment of human pro-opiomelanocortin in the human pituitary gland and in neoplasms</atitle><jtitle>Virchows Archiv A Pathological Anatomy and Histopathology</jtitle><addtitle>Virchows Arch A Pathol Anat Histopathol</addtitle><date>1985</date><risdate>1985</risdate><volume>408</volume><issue>2-3</issue><spage>281</spage><epage>287</epage><pages>281-287</pages><issn>0174-7398</issn><eissn>1432-2307</eissn><coden>VAAHDJ</coden><abstract>Immunohistochemical localization of the N-terminal fragment (1-76) (NTF) of human pro-opiomelanocortin (POMC) was studied in human adult and fetal pituitary glands, as well as in pituitary adenomas associated with Cushing's syndrome and in ectopic ACTH-producing tumors. Comparison of localization between NTF and ACTH was performed using mirror sections. Our results indicated concomitant localization of NTF and ACTH in the same cells, not only in normal adult and fetal pituitaries but also in pituitary adenomas and ectopic ACTH producing tumours. Specificity of the NTF staining was confirmed by immunoabsorption. Negative staining of the bovine pituitary gland indicated the immunohistochemical localization of N-terminal (1-45) of human POMC as there is a known species difference in the sequence 1-45 between human and the bovine N-terminal fragment. Presence of NTF in cisterna of rough endoplasmic reticulum indicates its production by small cell carcinoma. These findings, together with the previous studies, suggest that the complete form of POMC is produced in the tumours as well as in normal pituitaries.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>3002013</pmid><doi>10.1007/BF00707990</doi><tpages>7</tpages></addata></record> |
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| subjects | Adenoma - metabolism Adenoma - ultrastructure Adult Biological and medical sciences Carcinoma, Small Cell - analysis Carcinoma, Small Cell - ultrastructure Fundamental and applied biological sciences. Psychology Histocytochemistry Humans Hypothalamus. Hypophysis. Epiphysis. Urophysis Immunochemistry Infant Lung Neoplasms - analysis Lung Neoplasms - ultrastructure Microscopy, Electron Morphology. Functional localizations Peptide Fragments - analysis Pituitary Gland - analysis Pituitary Neoplasms - metabolism Pituitary Neoplasms - ultrastructure Pro-Opiomelanocortin - analysis Vertebrates: endocrinology |
| title | Light and electron microscopic localization of the N-terminal fragment of human pro-opiomelanocortin in the human pituitary gland and in neoplasms |
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