A simple fixation procedure for immunofluorescent detection of different cytoskeletal components within the same cell

In recent studies on the cytoskeletal organization of T51B rat liver cells by indirect immunofluorescence microscopy, we have been unable to achieve double-staining of microtubules and intermediate filaments within the same cell. In acetone-fixed cells, microtubules were poorly preserved, and two ou...

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Veröffentlicht in:	Histochemistry 1989, Vol.91 (1), p.81-88
Hauptverfasser:	VIELKIND, U, SWIERENGA, S. H
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - analysis Animals Biological and medical sciences Cells, Cultured Cytoskeleton - analysis Diverse techniques Fixatives Formaldehyde Fundamental and applied biological sciences. Psychology Immunohistochemistry - methods Intermediate Filaments - analysis Liver - analysis Liver - cytology Microtubules - analysis Molecular and cellular biology Rats
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