Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum
Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H2O2 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than t...
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| Veröffentlicht in: | Histochemistry 1979, Vol.64 (3), p.315-318 |
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| container_end_page | 318 |
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| container_title | Histochemistry |
| container_volume | 64 |
| creator | Christie, K N Stoward, P J |
| description | Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H2O2 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than to a non-enzymic binding reaction. The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium. |
| doi_str_mv | 10.1007/BF00495032 |
| format | Article |
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The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium.</description><identifier>ISSN: 0301-5564</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/BF00495032</identifier><identifier>PMID: 93104</identifier><language>eng</language><publisher>Germany</publisher><subject>Animals ; Cricetinae ; Histocytochemistry ; Muscles - enzymology ; Muscles - ultrastructure ; Peroxidases - analysis ; Sarcoplasmic Reticulum - enzymology ; Sarcoplasmic Reticulum - ultrastructure ; Staining and Labeling</subject><ispartof>Histochemistry, 1979, Vol.64 (3), p.315-318</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-33fc6f83c467fdda29cfe789192158f2b21701d42add16c57444c7ee6aea74383</citedby><cites>FETCH-LOGICAL-c280t-33fc6f83c467fdda29cfe789192158f2b21701d42add16c57444c7ee6aea74383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/93104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Christie, K N</creatorcontrib><creatorcontrib>Stoward, P J</creatorcontrib><title>Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum</title><title>Histochemistry</title><addtitle>Histochemistry</addtitle><description>Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H2O2 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than to a non-enzymic binding reaction. The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium.</description><subject>Animals</subject><subject>Cricetinae</subject><subject>Histocytochemistry</subject><subject>Muscles - enzymology</subject><subject>Muscles - ultrastructure</subject><subject>Peroxidases - analysis</subject><subject>Sarcoplasmic Reticulum - enzymology</subject><subject>Sarcoplasmic Reticulum - ultrastructure</subject><subject>Staining and Labeling</subject><issn>0301-5564</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1Lw0AQQBexaK1evHrZkwchul_JJkctVoWCBxW8he1ktl1JunE3EfvvTW3R08Dw5jE8Qs45u-aM6Zu7GWOqSJkUB2TMlRQJ58X7IRkzyXiSppk6JicxfjCWMa3zIzIqJGdqTJYvLYKzDly3od5S2HQeVtg4MDVtgwes-oCRWh9o7Yeli269pC0G_-0qE5Ea6NzX9tqtabdCGk0A39YmDg4asHPQ131zSkbW1BHP9nNC3mb3r9PHZP788DS9nScgctYlUlrIbC5BZdpWlREFWNR5wQvB09yKheCa8UoJU1U8g1QrpUAjZgaNVjKXE3K58w6_f_YYu7JxEbCuzRp9H0ut8iIVqRjAqx0IwccY0JZtcI0Jm5Kzctu0_G86wBd7a79osPpDfyPKH0mScyI</recordid><startdate>1979</startdate><enddate>1979</enddate><creator>Christie, K N</creator><creator>Stoward, P J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1979</creationdate><title>Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum</title><author>Christie, K N ; Stoward, P J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c280t-33fc6f83c467fdda29cfe789192158f2b21701d42add16c57444c7ee6aea74383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Animals</topic><topic>Cricetinae</topic><topic>Histocytochemistry</topic><topic>Muscles - enzymology</topic><topic>Muscles - ultrastructure</topic><topic>Peroxidases - analysis</topic><topic>Sarcoplasmic Reticulum - enzymology</topic><topic>Sarcoplasmic Reticulum - ultrastructure</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Christie, K N</creatorcontrib><creatorcontrib>Stoward, P J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Christie, K N</au><au>Stoward, P J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum</atitle><jtitle>Histochemistry</jtitle><addtitle>Histochemistry</addtitle><date>1979</date><risdate>1979</risdate><volume>64</volume><issue>3</issue><spage>315</spage><epage>318</epage><pages>315-318</pages><issn>0301-5564</issn><eissn>1432-119X</eissn><abstract>Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H2O2 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than to a non-enzymic binding reaction. The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium.</abstract><cop>Germany</cop><pmid>93104</pmid><doi>10.1007/BF00495032</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-5564 |
| ispartof | Histochemistry, 1979, Vol.64 (3), p.315-318 |
| issn | 0301-5564 1432-119X |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF00495032 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Cricetinae Histocytochemistry Muscles - enzymology Muscles - ultrastructure Peroxidases - analysis Sarcoplasmic Reticulum - enzymology Sarcoplasmic Reticulum - ultrastructure Staining and Labeling |
| title | Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum |
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