Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections: an electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens
A simple method is described allowing the enhancement of the visibility of small gold probes for the electron microscopy. This method, which allows the silver intensification of gold directly on epon-embedded ultrathin sections, was used for the electron microscopic localization of Mouse Mammary Tum...
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| Veröffentlicht in: | Histochemistry 1987-01, Vol.86 (4), p.371-373 |
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| container_end_page | 373 |
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| container_title | Histochemistry |
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| creator | MARCHETTI, A BISTOCCHI, M TOGNETTI, A. R |
| description | A simple method is described allowing the enhancement of the visibility of small gold probes for the electron microscopy. This method, which allows the silver intensification of gold directly on epon-embedded ultrathin sections, was used for the electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens in cultured cells derived from GR and BALB/cfRIII mouse mammary tumors. After the immunostaining with the preembedding protein A-gold technique, the ultrathin sections, placed on 200 mesh copper grids, were rehydrated and exposed to a photographic developer containing silver nitrate. During this physical development gold particles are incapsulated in growing shells of metallic silver, which gradually become more and more visible. We were able to obtain a heavy labelling of the viral particles, well visible even at low magnification, with a negligeable background staining. The present technique can be useful whenever it is necessary to use the smallest gold probes today available. |
| doi_str_mv | 10.1007/BF00494995 |
| format | Article |
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We were able to obtain a heavy labelling of the viral particles, well visible even at low magnification, with a negligeable background staining. The present technique can be useful whenever it is necessary to use the smallest gold probes today available.</description><identifier>ISSN: 0301-5564</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/BF00494995</identifier><identifier>PMID: 2437083</identifier><identifier>CODEN: HCMYAL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Antigens, Viral, Tumor - analysis ; Biological and medical sciences ; Cells, Cultured ; Fundamental and applied biological sciences. 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R</creatorcontrib><title>Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections: an electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens</title><title>Histochemistry</title><addtitle>Histochemistry</addtitle><description>A simple method is described allowing the enhancement of the visibility of small gold probes for the electron microscopy. This method, which allows the silver intensification of gold directly on epon-embedded ultrathin sections, was used for the electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens in cultured cells derived from GR and BALB/cfRIII mouse mammary tumors. After the immunostaining with the preembedding protein A-gold technique, the ultrathin sections, placed on 200 mesh copper grids, were rehydrated and exposed to a photographic developer containing silver nitrate. During this physical development gold particles are incapsulated in growing shells of metallic silver, which gradually become more and more visible. We were able to obtain a heavy labelling of the viral particles, well visible even at low magnification, with a negligeable background staining. The present technique can be useful whenever it is necessary to use the smallest gold probes today available.</description><subject>Animals</subject><subject>Antigens, Viral, Tumor - analysis</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold</subject><subject>Immunologic Techniques</subject><subject>Mammary Neoplasms, Experimental - immunology</subject><subject>Mammary Tumor Virus, Mouse - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Microscopy, Electron - methods</subject><subject>Silver</subject><subject>Staining and Labeling</subject><subject>Staphylococcal Protein A</subject><subject>Techniques used in virology</subject><subject>Virology</subject><issn>0301-5564</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkV9rFTEQxUNR6rXtS9-FPBRRYWuyyd4kvtViVejFB6-lb0v-zLYp2eSa7Ar6dfyiZumlPg3D_DgzZw5Cp5ScU0LE-49XhHDFleoO0Ipy1jaUqttnaEUYoU3XrfkL9LKUB0LWRAh5iA5bzgSRbIX-fvfhF2QM8V5HCyPECacB73KawEd80dyl4JbWQMEp4gzFxwZGA86Bw3OYsp7uK1nATj7F8gHriCHULld89DanYtPOWxyS1cH_0Qu27NikuQDe6HHU-TfezmPK-MbnueA3m8325m0VmvwdxHKMng86FDjZ1yP04-rT9vJLc_3t89fLi-vGtoJMTatZa5w0jppBUaGt404qkPU1Le8ok3otpBKSWQpUGsUE1W7gYq2MMlJ27Ai9ftStdn_OUKZ-9MVCCDpCvbUXgkvVMl7Bd4_gYq5kGPpd9ouLnpJ-SaT_n0iFX-1VZzOCe0L3EdT52X6uS33QkGsOvjxhgvO6tmX_ANpmlOQ</recordid><startdate>19870101</startdate><enddate>19870101</enddate><creator>MARCHETTI, A</creator><creator>BISTOCCHI, M</creator><creator>TOGNETTI, A. R</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19870101</creationdate><title>Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections: an electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens</title><author>MARCHETTI, A ; BISTOCCHI, M ; TOGNETTI, A. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-2a32bd8bd1bf917acd4d89e8049245138a6789783c1e18b9371adf4769b9b8853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Antigens, Viral, Tumor - analysis</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold</topic><topic>Immunologic Techniques</topic><topic>Mammary Neoplasms, Experimental - immunology</topic><topic>Mammary Tumor Virus, Mouse - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Microscopy, Electron - methods</topic><topic>Silver</topic><topic>Staining and Labeling</topic><topic>Staphylococcal Protein A</topic><topic>Techniques used in virology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MARCHETTI, A</creatorcontrib><creatorcontrib>BISTOCCHI, M</creatorcontrib><creatorcontrib>TOGNETTI, A. R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MARCHETTI, A</au><au>BISTOCCHI, M</au><au>TOGNETTI, A. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections: an electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens</atitle><jtitle>Histochemistry</jtitle><addtitle>Histochemistry</addtitle><date>1987-01-01</date><risdate>1987</risdate><volume>86</volume><issue>4</issue><spage>371</spage><epage>373</epage><pages>371-373</pages><issn>0301-5564</issn><eissn>1432-119X</eissn><coden>HCMYAL</coden><abstract>A simple method is described allowing the enhancement of the visibility of small gold probes for the electron microscopy. This method, which allows the silver intensification of gold directly on epon-embedded ultrathin sections, was used for the electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens in cultured cells derived from GR and BALB/cfRIII mouse mammary tumors. After the immunostaining with the preembedding protein A-gold technique, the ultrathin sections, placed on 200 mesh copper grids, were rehydrated and exposed to a photographic developer containing silver nitrate. During this physical development gold particles are incapsulated in growing shells of metallic silver, which gradually become more and more visible. We were able to obtain a heavy labelling of the viral particles, well visible even at low magnification, with a negligeable background staining. The present technique can be useful whenever it is necessary to use the smallest gold probes today available.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2437083</pmid><doi>10.1007/BF00494995</doi><tpages>3</tpages></addata></record> |
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| ispartof | Histochemistry, 1987-01, Vol.86 (4), p.371-373 |
| issn | 0301-5564 1432-119X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Antigens, Viral, Tumor - analysis Biological and medical sciences Cells, Cultured Fundamental and applied biological sciences. Psychology Gold Immunologic Techniques Mammary Neoplasms, Experimental - immunology Mammary Tumor Virus, Mouse - immunology Mice Mice, Inbred BALB C Microbiology Microscopy, Electron - methods Silver Staining and Labeling Staphylococcal Protein A Techniques used in virology Virology |
| title | Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections: an electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) antigens |
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