Localization of inorganic ions by precipitative freeze dissolution

Localization of inorganic ions by precipitative freeze dissolution

A new method for localization of inorganic diffusible ions in tissue is introduced. It has been applied to localization of Tl+ and Rb+ in barley roots and is probably also suited for Cs+, Ca2+, Cl-, Br-, PO 3/4- and perhaps K+. Its principle consists of dissolution of the ice from frozen tissue in a...

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Veröffentlicht in:Histochemistry 1978-06, Vol.55 (2), p.81-96
Hauptverfasser: van Iren, F, Bange, G G
Format: Artikel
Sprache:eng
Schlagworte:
Anions
Cations
Freezing
Histocytochemistry
Hordeum - metabolism
Methods
Plants - metabolism
Plants - ultrastructure
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Zusammenfassung:A new method for localization of inorganic diffusible ions in tissue is introduced. It has been applied to localization of Tl+ and Rb+ in barley roots and is probably also suited for Cs+, Ca2+, Cl-, Br-, PO 3/4- and perhaps K+. Its principle consists of dissolution of the ice from frozen tissue in a concentrated aqueous solution of a precipitating agent that is kept at a temperature just above its melting point.
ISSN:0301-5564
1432-119X
DOI:10.1007/BF00493511