A comparative study of PAS-phosphotungstic acid-diamine supra blue FGL and immunological reactions for type I collagen
Immunohistochemical techniques proved valuable in histological studies of various types of collagens. However drawbacks include non-specific reactions of antibodies, masking of antigens, and the high cost of antibodies. This study was undertaken to ascertain the specificity of the PAS-phosphotungsti...
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| Veröffentlicht in: | Histochemistry 1989-01, Vol.91 (4), p.283-289 |
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| container_title | Histochemistry |
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| creator | RAO, R. N FALLMAN, P. M FALLS, D. G MELOAN, S. N |
| description | Immunohistochemical techniques proved valuable in histological studies of various types of collagens. However drawbacks include non-specific reactions of antibodies, masking of antigens, and the high cost of antibodies. This study was undertaken to ascertain the specificity of the PAS-phosphotungstic acid-Diamine Supra Blue FGL (PAS-PTA-DSB-FGL) reaction for type I collagen, differentiating it from other collagens. Duplicate series of methacarn-fixed sections of various tissues were treated with the PAS-PTA-DSB FGL reaction and the peroxidase-antiperoxidase (PAP) technique for type I collagen and the staining patterns were compared. Fibers binding the blue dye were found only at sites reacting with antibodies against type I collagen. These observations indicate that the PAS-PTA-DSB FGL procedure is suitable for visualization of type I collagen, e.g. in screening of large series of sections and in the practice of surgical and autopsy pathology. |
| doi_str_mv | 10.1007/BF00493002 |
| format | Article |
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Duplicate series of methacarn-fixed sections of various tissues were treated with the PAS-PTA-DSB FGL reaction and the peroxidase-antiperoxidase (PAP) technique for type I collagen and the staining patterns were compared. Fibers binding the blue dye were found only at sites reacting with antibodies against type I collagen. These observations indicate that the PAS-PTA-DSB FGL procedure is suitable for visualization of type I collagen, e.g. in screening of large series of sections and in the practice of surgical and autopsy pathology.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Bronchi - metabolism</subject><subject>Collagen - metabolism</subject><subject>Fixatives</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kidney - metabolism</subject><subject>Liver - metabolism</subject><subject>Proteins</subject><subject>Spleen - metabolism</subject><subject>Staining and Labeling</subject><issn>0301-5564</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LxDAQhoMoun5cvAu56EGoTvqV5rgu7rqwoKCCt5KmkzXSNjVphf33Rix6GObwPrwzPIScM7hhAPz2bgmQigQg3iMzliZxxJh42yczSIBFWZanR-TY-w-AHDgvDslhnHLGgc3I15wq2_bSycF8IfXDWO-o1fRp_hz179aHGcZu6wejqFSmjmojW9MFcuydpFUzIl2uNlR2NTVtO3a2sVujZEMdSjUY23mqraPDrke6DreaRm6xOyUHWjYez6Z9Ql6X9y-Lh2jzuFov5ptIJSweIoEFU3HCsyqtuNCsKjJdFJjVUEPFucgQcwQWa8wEioyBzKUQklc51yxQyQm5-u3tnf0c0Q9la7zC8ESHdvQlF8AKztMAXv-CylnvHeqyd6aVblcyKH8kl_-SA3wxtY5Vi_UfOlkN-eWUSx9UaCc7Zfx_o0jyIuUi-QZi6oRF</recordid><startdate>19890101</startdate><enddate>19890101</enddate><creator>RAO, R. 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N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparative study of PAS-phosphotungstic acid-diamine supra blue FGL and immunological reactions for type I collagen</atitle><jtitle>Histochemistry</jtitle><addtitle>Histochemistry</addtitle><date>1989-01-01</date><risdate>1989</risdate><volume>91</volume><issue>4</issue><spage>283</spage><epage>289</epage><pages>283-289</pages><issn>0301-5564</issn><eissn>1432-119X</eissn><coden>HCMYAL</coden><abstract>Immunohistochemical techniques proved valuable in histological studies of various types of collagens. However drawbacks include non-specific reactions of antibodies, masking of antigens, and the high cost of antibodies. This study was undertaken to ascertain the specificity of the PAS-phosphotungstic acid-Diamine Supra Blue FGL (PAS-PTA-DSB-FGL) reaction for type I collagen, differentiating it from other collagens. Duplicate series of methacarn-fixed sections of various tissues were treated with the PAS-PTA-DSB FGL reaction and the peroxidase-antiperoxidase (PAP) technique for type I collagen and the staining patterns were compared. Fibers binding the blue dye were found only at sites reacting with antibodies against type I collagen. These observations indicate that the PAS-PTA-DSB FGL procedure is suitable for visualization of type I collagen, e.g. in screening of large series of sections and in the practice of surgical and autopsy pathology.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>2471701</pmid><doi>10.1007/BF00493002</doi><tpages>7</tpages></addata></record> |
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| ispartof | Histochemistry, 1989-01, Vol.91 (4), p.283-289 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Bronchi - metabolism Collagen - metabolism Fixatives Fundamental and applied biological sciences. Psychology Glycoproteins Humans Immunohistochemistry Kidney - metabolism Liver - metabolism Proteins Spleen - metabolism Staining and Labeling |
| title | A comparative study of PAS-phosphotungstic acid-diamine supra blue FGL and immunological reactions for type I collagen |
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