Dissimilar responsiveness of cultured corticotrophs and melanotrophs to tripeptide aldehydes
Cultured cells from adult rat anterior pituitaries or intermediate lobes were treated with the proteinase inhibitor tripeptide aldehydes BOC-DPhe-Pro-Arg-H (Boc-fPRH) and DPhe-Pro-Arg-H (fPRH), ovine corticotropin-releasing factor (oCRF), and bromocriptine. One millimolar fPRH stimulated basal, and...
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| Veröffentlicht in: | Histochemistry 1986-01, Vol.84 (4-6), p.418-422 |
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| container_title | Histochemistry |
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| creator | FAZEKAS, I RAPPAY, G BACSY, E MEDZIHRADSZKY-SCHWEIGER, H GYEVAI, A GAAL, G |
| description | Cultured cells from adult rat anterior pituitaries or intermediate lobes were treated with the proteinase inhibitor tripeptide aldehydes BOC-DPhe-Pro-Arg-H (Boc-fPRH) and DPhe-Pro-Arg-H (fPRH), ovine corticotropin-releasing factor (oCRF), and bromocriptine. One millimolar fPRH stimulated basal, and slightly enhanced oCRF-induced ACTH release by melanotrophs in short-term experiments. The basal release of alpha-MSH was also stimulated by the drug. In long-term experiments, fPRH elevated markedly both the release and the intracellular level of ACTH; BOC-fPRH caused an increased alpha-MSH release. Tritiated fPRH had no preference for POMC-producing cells and BOC-fPRH or fPRH were harmless to the cell morphology. In anterior pituitary cell cultures, fPRH diminished slightly basal and oCRF-induced ACTH release. Bromocriptine was ineffective on corticotrophs, however, in melanotrophs it inhibited ACTH release markedly with or without fPRH in the medium. The dissimilar responsiveness of the corticotrophs and melanotrophs to the peptide aldehydes may be interpreted in terms of their differing membrane receptors or intracellular mechanism of stimulus-secretion coupling. |
| doi_str_mv | 10.1007/BF00482972 |
| format | Article |
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One millimolar fPRH stimulated basal, and slightly enhanced oCRF-induced ACTH release by melanotrophs in short-term experiments. The basal release of alpha-MSH was also stimulated by the drug. In long-term experiments, fPRH elevated markedly both the release and the intracellular level of ACTH; BOC-fPRH caused an increased alpha-MSH release. Tritiated fPRH had no preference for POMC-producing cells and BOC-fPRH or fPRH were harmless to the cell morphology. In anterior pituitary cell cultures, fPRH diminished slightly basal and oCRF-induced ACTH release. Bromocriptine was ineffective on corticotrophs, however, in melanotrophs it inhibited ACTH release markedly with or without fPRH in the medium. The dissimilar responsiveness of the corticotrophs and melanotrophs to the peptide aldehydes may be interpreted in terms of their differing membrane receptors or intracellular mechanism of stimulus-secretion coupling.</description><identifier>ISSN: 0301-5564</identifier><identifier>EISSN: 1432-119X</identifier><identifier>DOI: 10.1007/BF00482972</identifier><identifier>PMID: 3013810</identifier><identifier>CODEN: HCMYAL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Adrenocorticotropic Hormone - metabolism ; Aldehydes - pharmacology ; Animals ; Biological and medical sciences ; Cells, Cultured ; Corticotropin-Releasing Hormone - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Hormones - pharmacology ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Melanocyte-Stimulating Hormones - metabolism ; Microscopy, Electron ; Oligopeptides - pharmacology ; Pituitary Gland - drug effects ; Pituitary Gland - metabolism ; Pituitary Gland - ultrastructure ; Pituitary Gland, Anterior - drug effects ; Pituitary Gland, Anterior - metabolism ; Pituitary Gland, Anterior - ultrastructure ; Protease Inhibitors - pharmacology ; Rats ; Secretory Rate - drug effects ; Vertebrates: endocrinology</subject><ispartof>Histochemistry, 1986-01, Vol.84 (4-6), p.418-422</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-a599012e580ad944ce84ae33603cd80a55716eb2d03acd86dcfd1c22cab722923</citedby><cites>FETCH-LOGICAL-c311t-a599012e580ad944ce84ae33603cd80a55716eb2d03acd86dcfd1c22cab722923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7999721$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3013810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FAZEKAS, I</creatorcontrib><creatorcontrib>RAPPAY, G</creatorcontrib><creatorcontrib>BACSY, E</creatorcontrib><creatorcontrib>MEDZIHRADSZKY-SCHWEIGER, H</creatorcontrib><creatorcontrib>GYEVAI, A</creatorcontrib><creatorcontrib>GAAL, G</creatorcontrib><title>Dissimilar responsiveness of cultured corticotrophs and melanotrophs to tripeptide aldehydes</title><title>Histochemistry</title><addtitle>Histochemistry</addtitle><description>Cultured cells from adult rat anterior pituitaries or intermediate lobes were treated with the proteinase inhibitor tripeptide aldehydes BOC-DPhe-Pro-Arg-H (Boc-fPRH) and DPhe-Pro-Arg-H (fPRH), ovine corticotropin-releasing factor (oCRF), and bromocriptine. One millimolar fPRH stimulated basal, and slightly enhanced oCRF-induced ACTH release by melanotrophs in short-term experiments. The basal release of alpha-MSH was also stimulated by the drug. In long-term experiments, fPRH elevated markedly both the release and the intracellular level of ACTH; BOC-fPRH caused an increased alpha-MSH release. Tritiated fPRH had no preference for POMC-producing cells and BOC-fPRH or fPRH were harmless to the cell morphology. In anterior pituitary cell cultures, fPRH diminished slightly basal and oCRF-induced ACTH release. Bromocriptine was ineffective on corticotrophs, however, in melanotrophs it inhibited ACTH release markedly with or without fPRH in the medium. The dissimilar responsiveness of the corticotrophs and melanotrophs to the peptide aldehydes may be interpreted in terms of their differing membrane receptors or intracellular mechanism of stimulus-secretion coupling.</description><subject>Adrenocorticotropic Hormone - metabolism</subject><subject>Aldehydes - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Corticotropin-Releasing Hormone - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones - pharmacology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Melanocyte-Stimulating Hormones - metabolism</subject><subject>Microscopy, Electron</subject><subject>Oligopeptides - pharmacology</subject><subject>Pituitary Gland - drug effects</subject><subject>Pituitary Gland - metabolism</subject><subject>Pituitary Gland - ultrastructure</subject><subject>Pituitary Gland, Anterior - drug effects</subject><subject>Pituitary Gland, Anterior - metabolism</subject><subject>Pituitary Gland, Anterior - ultrastructure</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Rats</subject><subject>Secretory Rate - drug effects</subject><subject>Vertebrates: endocrinology</subject><issn>0301-5564</issn><issn>1432-119X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LxDAQhYMo67p68S70IB6E6iTprxx1dVVY8KLgQSjZZMpG2qZmWmH_eyu76mngex-P4TF2yuGKA-TXtwuApBAqF3tsyhMpYs7V2z6bggQep2mWHLIjog-ADPK8mLDJyGXBYcre7xyRa1ytQxSQOt-S-8IWiSJfRWao-yGgjYwPvTO-D75bU6RbGzVY6_YX9D7qg-uw653FSNcW1xuLdMwOKl0TnuzujL0u7l_mj_Hy-eFpfrOMjeS8j3WqFHCBaQHaqiQxWCQapcxAGjuyNM15hithQeoRZNZUlhshjF7lQighZ-xi29sF_zkg9WXjyGA9foh-oDLPFEiuslG83IomeKKAVdkF1-iwKTmUP1OW_1OO8tmudVg1aP_U3XZjfr7LNRldV0G3xtGflis1tnD5DYX2fMU</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>FAZEKAS, I</creator><creator>RAPPAY, G</creator><creator>BACSY, E</creator><creator>MEDZIHRADSZKY-SCHWEIGER, H</creator><creator>GYEVAI, A</creator><creator>GAAL, G</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19860101</creationdate><title>Dissimilar responsiveness of cultured corticotrophs and melanotrophs to tripeptide aldehydes</title><author>FAZEKAS, I ; RAPPAY, G ; BACSY, E ; MEDZIHRADSZKY-SCHWEIGER, H ; GYEVAI, A ; GAAL, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-a599012e580ad944ce84ae33603cd80a55716eb2d03acd86dcfd1c22cab722923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Adrenocorticotropic Hormone - metabolism</topic><topic>Aldehydes - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Corticotropin-Releasing Hormone - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones - pharmacology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Melanocyte-Stimulating Hormones - metabolism</topic><topic>Microscopy, Electron</topic><topic>Oligopeptides - pharmacology</topic><topic>Pituitary Gland - drug effects</topic><topic>Pituitary Gland - metabolism</topic><topic>Pituitary Gland - ultrastructure</topic><topic>Pituitary Gland, Anterior - drug effects</topic><topic>Pituitary Gland, Anterior - metabolism</topic><topic>Pituitary Gland, Anterior - ultrastructure</topic><topic>Protease Inhibitors - pharmacology</topic><topic>Rats</topic><topic>Secretory Rate - drug effects</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FAZEKAS, I</creatorcontrib><creatorcontrib>RAPPAY, G</creatorcontrib><creatorcontrib>BACSY, E</creatorcontrib><creatorcontrib>MEDZIHRADSZKY-SCHWEIGER, H</creatorcontrib><creatorcontrib>GYEVAI, A</creatorcontrib><creatorcontrib>GAAL, G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Histochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FAZEKAS, I</au><au>RAPPAY, G</au><au>BACSY, E</au><au>MEDZIHRADSZKY-SCHWEIGER, H</au><au>GYEVAI, A</au><au>GAAL, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissimilar responsiveness of cultured corticotrophs and melanotrophs to tripeptide aldehydes</atitle><jtitle>Histochemistry</jtitle><addtitle>Histochemistry</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>84</volume><issue>4-6</issue><spage>418</spage><epage>422</epage><pages>418-422</pages><issn>0301-5564</issn><eissn>1432-119X</eissn><coden>HCMYAL</coden><abstract>Cultured cells from adult rat anterior pituitaries or intermediate lobes were treated with the proteinase inhibitor tripeptide aldehydes BOC-DPhe-Pro-Arg-H (Boc-fPRH) and DPhe-Pro-Arg-H (fPRH), ovine corticotropin-releasing factor (oCRF), and bromocriptine. One millimolar fPRH stimulated basal, and slightly enhanced oCRF-induced ACTH release by melanotrophs in short-term experiments. The basal release of alpha-MSH was also stimulated by the drug. In long-term experiments, fPRH elevated markedly both the release and the intracellular level of ACTH; BOC-fPRH caused an increased alpha-MSH release. Tritiated fPRH had no preference for POMC-producing cells and BOC-fPRH or fPRH were harmless to the cell morphology. In anterior pituitary cell cultures, fPRH diminished slightly basal and oCRF-induced ACTH release. Bromocriptine was ineffective on corticotrophs, however, in melanotrophs it inhibited ACTH release markedly with or without fPRH in the medium. The dissimilar responsiveness of the corticotrophs and melanotrophs to the peptide aldehydes may be interpreted in terms of their differing membrane receptors or intracellular mechanism of stimulus-secretion coupling.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>3013810</pmid><doi>10.1007/BF00482972</doi><tpages>5</tpages></addata></record> |
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| ispartof | Histochemistry, 1986-01, Vol.84 (4-6), p.418-422 |
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| subjects | Adrenocorticotropic Hormone - metabolism Aldehydes - pharmacology Animals Biological and medical sciences Cells, Cultured Corticotropin-Releasing Hormone - pharmacology Female Fundamental and applied biological sciences. Psychology Hormones - pharmacology Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Melanocyte-Stimulating Hormones - metabolism Microscopy, Electron Oligopeptides - pharmacology Pituitary Gland - drug effects Pituitary Gland - metabolism Pituitary Gland - ultrastructure Pituitary Gland, Anterior - drug effects Pituitary Gland, Anterior - metabolism Pituitary Gland, Anterior - ultrastructure Protease Inhibitors - pharmacology Rats Secretory Rate - drug effects Vertebrates: endocrinology |
| title | Dissimilar responsiveness of cultured corticotrophs and melanotrophs to tripeptide aldehydes |
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