Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2
A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin o...
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| Veröffentlicht in: | Archives of microbiology 1984-04, Vol.137 (4), p.357-361 |
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| container_title | Archives of microbiology |
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| creator | PFEIFFER, P RADLER, F |
| description | A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr = 16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins. |
| doi_str_mv | 10.1007/BF00410734 |
| format | Article |
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Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr = 16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/BF00410734</identifier><identifier>PMID: 6375620</identifier><identifier>CODEN: AMICCW</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Amino Acids - analysis ; Biological and medical sciences ; Candida - chemistry ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Hydrogen-Ion Concentration ; Isoelectric Point ; Killer Factors, Yeast ; Microbiology ; Molecular Weight ; Mycology ; Mycotoxins - isolation & purification ; Mycotoxins - pharmacology ; Pichia - chemistry ; Saccharomyces cerevisiae - chemistry ; Saccharomyces cerevisiae Proteins ; Species Specificity ; Temperature ; Yeasts - drug effects</subject><ispartof>Archives of microbiology, 1984-04, Vol.137 (4), p.357-361</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-33adc4b39fa572a9557da060a5b8ce1ae2759aa7655dea14112bb70ceb2f064c3</citedby><cites>FETCH-LOGICAL-c334t-33adc4b39fa572a9557da060a5b8ce1ae2759aa7655dea14112bb70ceb2f064c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9673167$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6375620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PFEIFFER, P</creatorcontrib><creatorcontrib>RADLER, F</creatorcontrib><creatorcontrib>Kasetsart Univ., Bangkok (Thailand)</creatorcontrib><title>Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr = 16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.</description><subject>Amino Acids - analysis</subject><subject>Biological and medical sciences</subject><subject>Candida - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Isoelectric Point</subject><subject>Killer Factors, Yeast</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Mycology</subject><subject>Mycotoxins - isolation & purification</subject><subject>Mycotoxins - pharmacology</subject><subject>Pichia - chemistry</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Species Specificity</subject><subject>Temperature</subject><subject>Yeasts - drug effects</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDFPwzAQRi0EKqWwsIM8MCEFznYcJyOUFhCVWEBCYogujg2GtInsFNF_T2iqdjiddN_7bniEnDK4YgDq-nYKEDNQIt4jQxYLHoHib_tkCAJ4lGZCHJKjEL4AGE_TdEAGiVAy4TAk7-N63qB3oV7Q2tL209BvV1XG07b-detbMD_GY0VXBkMbKC7KNdYsvbNOY-v6Ku4a7aox9IkfkwOLVTAnmz0ir9PJy_ghmj3fP45vZpEWIm4jIbDUcSEyi1JxzKRUJUICKItUG4aGK5khqkTK0iCLGeNFoUCbgltIYi1G5LL_q30dgjc2b7ybo1_lDPJ_QflOUAef9XCzLOam3KIbI11-sckxaKysx4V2YYtliRKsmxE57zGLdY4fncD8bsKyVAJjSopU_AEL2HWm</recordid><startdate>198404</startdate><enddate>198404</enddate><creator>PFEIFFER, P</creator><creator>RADLER, F</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>198404</creationdate><title>Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2</title><author>PFEIFFER, P ; RADLER, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-33adc4b39fa572a9557da060a5b8ce1ae2759aa7655dea14112bb70ceb2f064c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Amino Acids - analysis</topic><topic>Biological and medical sciences</topic><topic>Candida - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Isoelectric Point</topic><topic>Killer Factors, Yeast</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Mycology</topic><topic>Mycotoxins - isolation & purification</topic><topic>Mycotoxins - pharmacology</topic><topic>Pichia - chemistry</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Species Specificity</topic><topic>Temperature</topic><topic>Yeasts - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PFEIFFER, P</creatorcontrib><creatorcontrib>RADLER, F</creatorcontrib><creatorcontrib>Kasetsart Univ., Bangkok (Thailand)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PFEIFFER, P</au><au>RADLER, F</au><aucorp>Kasetsart Univ., Bangkok (Thailand)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2</atitle><jtitle>Archives of microbiology</jtitle><addtitle>Arch Microbiol</addtitle><date>1984-04</date><risdate>1984</risdate><volume>137</volume><issue>4</issue><spage>357</spage><epage>361</epage><pages>357-361</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><coden>AMICCW</coden><abstract>A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr = 16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>6375620</pmid><doi>10.1007/BF00410734</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-8933 |
| ispartof | Archives of microbiology, 1984-04, Vol.137 (4), p.357-361 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF00410734 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amino Acids - analysis Biological and medical sciences Candida - chemistry Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hydrogen-Ion Concentration Isoelectric Point Killer Factors, Yeast Microbiology Molecular Weight Mycology Mycotoxins - isolation & purification Mycotoxins - pharmacology Pichia - chemistry Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae Proteins Species Specificity Temperature Yeasts - drug effects |
| title | Comparison of the killer toxin of several yeasts and the purification of a toxin of type K2 |
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