Analysis of centromere function in Saccharomyces cerevisiae using synthetic centromere mutants

We constructed Saccharides cerevisiae centromere DNA mutants by annealing and ligating synthetics oligonucleotides, a novel approach to centromere DNA mutagenesis that allowed us to change only one structural parameter at a time. Using this method, we confirmed that CDE I, II, and III alone are suff...

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Veröffentlicht in:	Chromosoma 1991-12, Vol.101 (3), p.189-197
Hauptverfasser:	Murphy, M.R, Fowlkes, D.M, Fitzgerald-Hayes, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Centromere - physiology Centromere - ultrastructure centromeres Chromosomes, Fungal - physiology Chromosomes, Fungal - ultrastructure Classical genetics, quantitative genetics, hybrids Consensus Sequence DNA DNA, Fungal - physiology Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution meiosis mitosis Molecular Sequence Data mutagenesis mutants Mutation nucleotide sequences plasmids Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae - ultrastructure segregation Thallophyta, bryophyta Vegetals
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