Veratridine blocks voltage-gated potassium current in human T lymphocytes and in mouse neuroblastoma cells
(i) Effects of veratridine on ionic conductances of human peripheral blood T lymphocytes have been investigated using the whole-cell patch-clamp technique. (ii) Veratridine reduces the net outward current evoked by membrane depolarizations. The reduction originates from block of a 4-aminopyridine-se...
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| Veröffentlicht in: | The Journal of membrane biology 1994-02, Vol.137 (3), p.205-214 |
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| container_title | The Journal of membrane biology |
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| creator | BERHEUGEN, J. A. H OORTGIESEN, M VIJVERBERG, H. P. M |
| description | (i) Effects of veratridine on ionic conductances of human peripheral blood T lymphocytes have been investigated using the whole-cell patch-clamp technique. (ii) Veratridine reduces the net outward current evoked by membrane depolarizations. The reduction originates from block of a 4-aminopyridine-sensitive, voltage-gated K+ current. (iii) Human T lymphocytes do not appear to express voltage-gated Na+ channels, since inward currents are observed neither in control nor in veratridine- and bretylium-exposed lymphocytes. (iv) The effect of veratridine consists of an increase in the rate of decay of the voltage-gated K+ current and a reduction of the peak current amplitude. Both effects depend on veratridine concentration. Half-maximum block occurs at 97 microM and the time constant of decay is reduced by 50% at 54 microM of veratridine. (v) Possible mechanisms of veratridine action are discussed. The increased rate of K+ current decay is most likely due to open channel block. The decrease of current amplitude may involve an additional mechanism. (vi) In cultured mouse neuroblastoma N1E-115 cells, veratridine blocks a component of voltage-gated K+ current, in addition to its effect on voltage-gated Na+ current. This result shows that the novel effect of veratridine is not confined to lymphocytes. |
| doi_str_mv | 10.1007/BF00232589 |
| format | Article |
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A. H ; OORTGIESEN, M ; VIJVERBERG, H. P. M</creator><creatorcontrib>BERHEUGEN, J. A. H ; OORTGIESEN, M ; VIJVERBERG, H. P. M</creatorcontrib><description>(i) Effects of veratridine on ionic conductances of human peripheral blood T lymphocytes have been investigated using the whole-cell patch-clamp technique. (ii) Veratridine reduces the net outward current evoked by membrane depolarizations. The reduction originates from block of a 4-aminopyridine-sensitive, voltage-gated K+ current. (iii) Human T lymphocytes do not appear to express voltage-gated Na+ channels, since inward currents are observed neither in control nor in veratridine- and bretylium-exposed lymphocytes. (iv) The effect of veratridine consists of an increase in the rate of decay of the voltage-gated K+ current and a reduction of the peak current amplitude. Both effects depend on veratridine concentration. Half-maximum block occurs at 97 microM and the time constant of decay is reduced by 50% at 54 microM of veratridine. (v) Possible mechanisms of veratridine action are discussed. The increased rate of K+ current decay is most likely due to open channel block. The decrease of current amplitude may involve an additional mechanism. (vi) In cultured mouse neuroblastoma N1E-115 cells, veratridine blocks a component of voltage-gated K+ current, in addition to its effect on voltage-gated Na+ current. This result shows that the novel effect of veratridine is not confined to lymphocytes.</description><identifier>ISSN: 0022-2631</identifier><identifier>EISSN: 1432-1424</identifier><identifier>DOI: 10.1007/BF00232589</identifier><identifier>PMID: 8182730</identifier><identifier>CODEN: JMBBBO</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>4-Aminopyridine - pharmacology ; Animals ; Biological and medical sciences ; Cadmium - pharmacology ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; Dose-Response Relationship, Drug ; Drug Interactions ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; Membrane Potentials ; Mice ; Molecular and cellular biology ; Neuroblastoma - metabolism ; Potassium - metabolism ; Potassium Channels - drug effects ; Potassium Channels - metabolism ; T-Lymphocytes - drug effects ; T-Lymphocytes - metabolism ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - metabolism ; Veratridine - administration & dosage ; Veratridine - pharmacology</subject><ispartof>The Journal of membrane biology, 1994-02, Vol.137 (3), p.205-214</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4159028$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8182730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BERHEUGEN, J. A. H</creatorcontrib><creatorcontrib>OORTGIESEN, M</creatorcontrib><creatorcontrib>VIJVERBERG, H. P. M</creatorcontrib><title>Veratridine blocks voltage-gated potassium current in human T lymphocytes and in mouse neuroblastoma cells</title><title>The Journal of membrane biology</title><addtitle>J Membr Biol</addtitle><description>(i) Effects of veratridine on ionic conductances of human peripheral blood T lymphocytes have been investigated using the whole-cell patch-clamp technique. (ii) Veratridine reduces the net outward current evoked by membrane depolarizations. The reduction originates from block of a 4-aminopyridine-sensitive, voltage-gated K+ current. (iii) Human T lymphocytes do not appear to express voltage-gated Na+ channels, since inward currents are observed neither in control nor in veratridine- and bretylium-exposed lymphocytes. (iv) The effect of veratridine consists of an increase in the rate of decay of the voltage-gated K+ current and a reduction of the peak current amplitude. Both effects depend on veratridine concentration. Half-maximum block occurs at 97 microM and the time constant of decay is reduced by 50% at 54 microM of veratridine. (v) Possible mechanisms of veratridine action are discussed. The increased rate of K+ current decay is most likely due to open channel block. The decrease of current amplitude may involve an additional mechanism. (vi) In cultured mouse neuroblastoma N1E-115 cells, veratridine blocks a component of voltage-gated K+ current, in addition to its effect on voltage-gated Na+ current. This result shows that the novel effect of veratridine is not confined to lymphocytes.</description><subject>4-Aminopyridine - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cadmium - pharmacology</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Interactions</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Membrane Potentials</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Neuroblastoma - metabolism</subject><subject>Potassium - metabolism</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - metabolism</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - metabolism</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Veratridine - administration & dosage</subject><subject>Veratridine - pharmacology</subject><issn>0022-2631</issn><issn>1432-1424</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LwzAUgIMoc04v3oUcPAnV_GrTHHU4FQZepteSpsnWmTQlSYX993ZszNOD9308eB8Atxg9YoT408sCIUJJXoozMMWMkgwzws7BdFyTjBQUX4KrGLcIYc4LNgGTEpeEUzQF228dZApt03Ya1tarnwh_vU1yrbO1TLqBvU8yxnZwUA0h6C7BtoObwckOrqDduX7j1S7pCGXX7JHzQ9Sw00PwtZUxeSeh0tbGa3BhpI365jhn4Gvxupq_Z8vPt4_58zJThKOUKU5ETpVipaamrLGgFGPSYKIaViJpBDO8EAwVBeGYCoG1KZSRiHPN8vFvOgMPh7sq-BiDNlUfWifDrsKo2veq_nuN8t1B7ofa6eakHgON_P7IZVTSmiA71caTxnAuECnpH6gYcms</recordid><startdate>19940201</startdate><enddate>19940201</enddate><creator>BERHEUGEN, J. A. H</creator><creator>OORTGIESEN, M</creator><creator>VIJVERBERG, H. P. M</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19940201</creationdate><title>Veratridine blocks voltage-gated potassium current in human T lymphocytes and in mouse neuroblastoma cells</title><author>BERHEUGEN, J. A. H ; OORTGIESEN, M ; VIJVERBERG, H. P. M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c270t-c72953cc48e3f8b1933112d12cd480af94f76940662713991ef6cfa077e451423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>4-Aminopyridine - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cadmium - pharmacology</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Interactions</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Membrane Potentials</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Neuroblastoma - metabolism</topic><topic>Potassium - metabolism</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - metabolism</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - metabolism</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - metabolism</topic><topic>Veratridine - administration & dosage</topic><topic>Veratridine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BERHEUGEN, J. A. H</creatorcontrib><creatorcontrib>OORTGIESEN, M</creatorcontrib><creatorcontrib>VIJVERBERG, H. P. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The Journal of membrane biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BERHEUGEN, J. A. H</au><au>OORTGIESEN, M</au><au>VIJVERBERG, H. P. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Veratridine blocks voltage-gated potassium current in human T lymphocytes and in mouse neuroblastoma cells</atitle><jtitle>The Journal of membrane biology</jtitle><addtitle>J Membr Biol</addtitle><date>1994-02-01</date><risdate>1994</risdate><volume>137</volume><issue>3</issue><spage>205</spage><epage>214</epage><pages>205-214</pages><issn>0022-2631</issn><eissn>1432-1424</eissn><coden>JMBBBO</coden><abstract>(i) Effects of veratridine on ionic conductances of human peripheral blood T lymphocytes have been investigated using the whole-cell patch-clamp technique. (ii) Veratridine reduces the net outward current evoked by membrane depolarizations. The reduction originates from block of a 4-aminopyridine-sensitive, voltage-gated K+ current. (iii) Human T lymphocytes do not appear to express voltage-gated Na+ channels, since inward currents are observed neither in control nor in veratridine- and bretylium-exposed lymphocytes. (iv) The effect of veratridine consists of an increase in the rate of decay of the voltage-gated K+ current and a reduction of the peak current amplitude. Both effects depend on veratridine concentration. Half-maximum block occurs at 97 microM and the time constant of decay is reduced by 50% at 54 microM of veratridine. (v) Possible mechanisms of veratridine action are discussed. The increased rate of K+ current decay is most likely due to open channel block. The decrease of current amplitude may involve an additional mechanism. (vi) In cultured mouse neuroblastoma N1E-115 cells, veratridine blocks a component of voltage-gated K+ current, in addition to its effect on voltage-gated Na+ current. This result shows that the novel effect of veratridine is not confined to lymphocytes.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>8182730</pmid><doi>10.1007/BF00232589</doi><tpages>10</tpages></addata></record> |
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| ispartof | The Journal of membrane biology, 1994-02, Vol.137 (3), p.205-214 |
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| subjects | 4-Aminopyridine - pharmacology Animals Biological and medical sciences Cadmium - pharmacology Cell membranes. Ionic channels. Membrane pores Cell structures and functions Dose-Response Relationship, Drug Drug Interactions Electrophysiology Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Membrane Potentials Mice Molecular and cellular biology Neuroblastoma - metabolism Potassium - metabolism Potassium Channels - drug effects Potassium Channels - metabolism T-Lymphocytes - drug effects T-Lymphocytes - metabolism Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism Veratridine - administration & dosage Veratridine - pharmacology |
| title | Veratridine blocks voltage-gated potassium current in human T lymphocytes and in mouse neuroblastoma cells |
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