Effects of microprojectile bombardment on embryogenic suspension cell cultures of maize (Zea mays L.) used for genetic transformation
We have investigated the interaction between tungsten and gold microprojectiles with suspension-culture cells of maize used for genetic transformation. Particle size measurements were evaluated before and after DNA precipitation to determine mean particle size and the effect of DNA precipitation on...
Gespeichert in:
| Veröffentlicht in: | Planta 1995, Vol.196 (3), p.501-509 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 509 |
|---|---|
| container_issue | 3 |
| container_start_page | 501 |
| container_title | Planta |
| container_volume | 196 |
| creator | Kausch, A.P Adams, T.R Mangano, M Zachwieja, S.J Gordon-Kamm, W Daines, R Willetts, N.G Chambers, S.A Adam, W. Jr Anderson, A Williams, G Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA)) |
| description | We have investigated the interaction between tungsten and gold microprojectiles with suspension-culture cells of maize used for genetic transformation. Particle size measurements were evaluated before and after DNA precipitation to determine mean particle size and the effect of DNA precipitation on particle aggregation. Following particle bombardment, metal foils were examined by scanning electron microscopy to visualize dispersion of individual particles and aggregates. Particle penetration into suspension-culture cell clusters was examined in paraffin-embedded bombarded cells serially sectioned and viewed with light microscopy and by energy dispersive X-ray microanalysis. Acridine-orange-stained bombarded cells were examined to observe cellular response to particle penetration. Transient expression of reporter genes C1 and B and GUS, (β-glucuronidase) were used to assess effects of particle bombardment on embryogenic cell types. Autoradiographic analysis of the transformable suspension cell culture SC82 (see Gordon-Kamm et al. 1990, Plant Cell 2, 603—618) was conducted to evaluate the S-phase and mitotic indices in embryogenic and nonembryogenic cells throughout a subculture passage and in response to DNA/particle delivery. The results of these investigations are discussed relative to cytodifferentiation of suspension cell clusters and recovery of transformed clonal sectors. |
| doi_str_mv | 10.1007/BF00203650 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1007_BF00203650</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>23383431</jstor_id><sourcerecordid>23383431</sourcerecordid><originalsourceid>FETCH-LOGICAL-c299t-3eced13a352f0d81ebf26da9a364321f5af8bbcef6740a617ee104b23659a92c3</originalsourceid><addsrcrecordid>eNpFUD1PwzAQtRBIlMLCiITkgQGQCmc7TZoRSvmQKrHAwhJdnHOVKokrOx3Kzv_mUBBMd3rv3bu7J8SpghsFkN3ePwJoMOkU9sRIJUZPNCSzfTEC4B5yMz0URzGuAZjMspH4WjhHto_SO9nWNvhN8GsG6oZk6dsSQ9VS10vfSWrLsPMr6mor4zZuqIs1w5aaRtpt028DDTZYf5K8_CDkdhfl8uZKbiNV0vkgeZx6NugDdpGBFns2ORYHDptIJ791LN4fF2_z58ny9ellfrecWJ3n_cSQpUoZNFPtoJopKp1OK8zRpPyqclN0s7K05NIsAUxVRqQgKTXnkWOurRmL68GXH40xkCs2oW4x7AoFxU-AxX-ALL4YxBuMFhvHF9s6_k2wRgPvHYvzQbaOvQ9_tDZmZhKjmD8beIe-wFVgi4dFnj6pLM3NN84Gg9o</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effects of microprojectile bombardment on embryogenic suspension cell cultures of maize (Zea mays L.) used for genetic transformation</title><source>Jstor Complete Legacy</source><source>SpringerLink Journals - AutoHoldings</source><creator>Kausch, A.P ; Adams, T.R ; Mangano, M ; Zachwieja, S.J ; Gordon-Kamm, W ; Daines, R ; Willetts, N.G ; Chambers, S.A ; Adam, W. Jr ; Anderson, A ; Williams, G ; Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA))</creator><creatorcontrib>Kausch, A.P ; Adams, T.R ; Mangano, M ; Zachwieja, S.J ; Gordon-Kamm, W ; Daines, R ; Willetts, N.G ; Chambers, S.A ; Adam, W. Jr ; Anderson, A ; Williams, G ; Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA))</creatorcontrib><description>We have investigated the interaction between tungsten and gold microprojectiles with suspension-culture cells of maize used for genetic transformation. Particle size measurements were evaluated before and after DNA precipitation to determine mean particle size and the effect of DNA precipitation on particle aggregation. Following particle bombardment, metal foils were examined by scanning electron microscopy to visualize dispersion of individual particles and aggregates. Particle penetration into suspension-culture cell clusters was examined in paraffin-embedded bombarded cells serially sectioned and viewed with light microscopy and by energy dispersive X-ray microanalysis. Acridine-orange-stained bombarded cells were examined to observe cellular response to particle penetration. Transient expression of reporter genes C1 and B and GUS, (β-glucuronidase) were used to assess effects of particle bombardment on embryogenic cell types. Autoradiographic analysis of the transformable suspension cell culture SC82 (see Gordon-Kamm et al. 1990, Plant Cell 2, 603—618) was conducted to evaluate the S-phase and mitotic indices in embryogenic and nonembryogenic cells throughout a subculture passage and in response to DNA/particle delivery. The results of these investigations are discussed relative to cytodifferentiation of suspension cell clusters and recovery of transformed clonal sectors.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/BF00203650</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; Biolistics ; Biological and medical sciences ; Biotechnology ; CELL CULTURE ; Cell culture techniques ; CELL DIFFERENTIATION ; Cell lines ; Chemical suspensions ; Corn ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; Cultured cells ; DIFERENCIACION CELULAR ; DIFFERENCIATION CELLULAIRE ; DNA ; Embryonic cells ; Fundamental and applied biological sciences. Psychology ; Genetic engineering applications ; GENETIC TRANSFORMATION ; Genetics and breeding of economic plants ; Genuebertragung ; Particle size classes ; Pflanzenbau ; Pflanzenzuechtung ; Plant breeding: fundamental aspects and methodology ; Plasmid ; PLASMIDE ; PLASMIDIOS ; PLASMIDS ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Tungsten ; Zea ; ZEA MAYS ; Zelldifferenzierung ; Zellkultur</subject><ispartof>Planta, 1995, Vol.196 (3), p.501-509</ispartof><rights>Springer-Verlag 1995</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c299t-3eced13a352f0d81ebf26da9a364321f5af8bbcef6740a617ee104b23659a92c3</citedby><cites>FETCH-LOGICAL-c299t-3eced13a352f0d81ebf26da9a364321f5af8bbcef6740a617ee104b23659a92c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23383431$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23383431$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,4010,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3652064$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kausch, A.P</creatorcontrib><creatorcontrib>Adams, T.R</creatorcontrib><creatorcontrib>Mangano, M</creatorcontrib><creatorcontrib>Zachwieja, S.J</creatorcontrib><creatorcontrib>Gordon-Kamm, W</creatorcontrib><creatorcontrib>Daines, R</creatorcontrib><creatorcontrib>Willetts, N.G</creatorcontrib><creatorcontrib>Chambers, S.A</creatorcontrib><creatorcontrib>Adam, W. Jr</creatorcontrib><creatorcontrib>Anderson, A</creatorcontrib><creatorcontrib>Williams, G</creatorcontrib><creatorcontrib>Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA))</creatorcontrib><title>Effects of microprojectile bombardment on embryogenic suspension cell cultures of maize (Zea mays L.) used for genetic transformation</title><title>Planta</title><description>We have investigated the interaction between tungsten and gold microprojectiles with suspension-culture cells of maize used for genetic transformation. Particle size measurements were evaluated before and after DNA precipitation to determine mean particle size and the effect of DNA precipitation on particle aggregation. Following particle bombardment, metal foils were examined by scanning electron microscopy to visualize dispersion of individual particles and aggregates. Particle penetration into suspension-culture cell clusters was examined in paraffin-embedded bombarded cells serially sectioned and viewed with light microscopy and by energy dispersive X-ray microanalysis. Acridine-orange-stained bombarded cells were examined to observe cellular response to particle penetration. Transient expression of reporter genes C1 and B and GUS, (β-glucuronidase) were used to assess effects of particle bombardment on embryogenic cell types. Autoradiographic analysis of the transformable suspension cell culture SC82 (see Gordon-Kamm et al. 1990, Plant Cell 2, 603—618) was conducted to evaluate the S-phase and mitotic indices in embryogenic and nonembryogenic cells throughout a subculture passage and in response to DNA/particle delivery. The results of these investigations are discussed relative to cytodifferentiation of suspension cell clusters and recovery of transformed clonal sectors.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biolistics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CELL CULTURE</subject><subject>Cell culture techniques</subject><subject>CELL DIFFERENTIATION</subject><subject>Cell lines</subject><subject>Chemical suspensions</subject><subject>Corn</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>Cultured cells</subject><subject>DIFERENCIACION CELULAR</subject><subject>DIFFERENCIATION CELLULAIRE</subject><subject>DNA</subject><subject>Embryonic cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering applications</subject><subject>GENETIC TRANSFORMATION</subject><subject>Genetics and breeding of economic plants</subject><subject>Genuebertragung</subject><subject>Particle size classes</subject><subject>Pflanzenbau</subject><subject>Pflanzenzuechtung</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plasmid</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>Tungsten</subject><subject>Zea</subject><subject>ZEA MAYS</subject><subject>Zelldifferenzierung</subject><subject>Zellkultur</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNpFUD1PwzAQtRBIlMLCiITkgQGQCmc7TZoRSvmQKrHAwhJdnHOVKokrOx3Kzv_mUBBMd3rv3bu7J8SpghsFkN3ePwJoMOkU9sRIJUZPNCSzfTEC4B5yMz0URzGuAZjMspH4WjhHto_SO9nWNvhN8GsG6oZk6dsSQ9VS10vfSWrLsPMr6mor4zZuqIs1w5aaRtpt028DDTZYf5K8_CDkdhfl8uZKbiNV0vkgeZx6NugDdpGBFns2ORYHDptIJ791LN4fF2_z58ny9ellfrecWJ3n_cSQpUoZNFPtoJopKp1OK8zRpPyqclN0s7K05NIsAUxVRqQgKTXnkWOurRmL68GXH40xkCs2oW4x7AoFxU-AxX-ALL4YxBuMFhvHF9s6_k2wRgPvHYvzQbaOvQ9_tDZmZhKjmD8beIe-wFVgi4dFnj6pLM3NN84Gg9o</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Kausch, A.P</creator><creator>Adams, T.R</creator><creator>Mangano, M</creator><creator>Zachwieja, S.J</creator><creator>Gordon-Kamm, W</creator><creator>Daines, R</creator><creator>Willetts, N.G</creator><creator>Chambers, S.A</creator><creator>Adam, W. Jr</creator><creator>Anderson, A</creator><creator>Williams, G</creator><creator>Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA))</creator><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1995</creationdate><title>Effects of microprojectile bombardment on embryogenic suspension cell cultures of maize (Zea mays L.) used for genetic transformation</title><author>Kausch, A.P ; Adams, T.R ; Mangano, M ; Zachwieja, S.J ; Gordon-Kamm, W ; Daines, R ; Willetts, N.G ; Chambers, S.A ; Adam, W. Jr ; Anderson, A ; Williams, G ; Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA))</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c299t-3eced13a352f0d81ebf26da9a364321f5af8bbcef6740a617ee104b23659a92c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biolistics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CELL CULTURE</topic><topic>Cell culture techniques</topic><topic>CELL DIFFERENTIATION</topic><topic>Cell lines</topic><topic>Chemical suspensions</topic><topic>Corn</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>Cultured cells</topic><topic>DIFERENCIACION CELULAR</topic><topic>DIFFERENCIATION CELLULAIRE</topic><topic>DNA</topic><topic>Embryonic cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering applications</topic><topic>GENETIC TRANSFORMATION</topic><topic>Genetics and breeding of economic plants</topic><topic>Genuebertragung</topic><topic>Particle size classes</topic><topic>Pflanzenbau</topic><topic>Pflanzenzuechtung</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plasmid</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>Tungsten</topic><topic>Zea</topic><topic>ZEA MAYS</topic><topic>Zelldifferenzierung</topic><topic>Zellkultur</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kausch, A.P</creatorcontrib><creatorcontrib>Adams, T.R</creatorcontrib><creatorcontrib>Mangano, M</creatorcontrib><creatorcontrib>Zachwieja, S.J</creatorcontrib><creatorcontrib>Gordon-Kamm, W</creatorcontrib><creatorcontrib>Daines, R</creatorcontrib><creatorcontrib>Willetts, N.G</creatorcontrib><creatorcontrib>Chambers, S.A</creatorcontrib><creatorcontrib>Adam, W. Jr</creatorcontrib><creatorcontrib>Anderson, A</creatorcontrib><creatorcontrib>Williams, G</creatorcontrib><creatorcontrib>Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA))</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kausch, A.P</au><au>Adams, T.R</au><au>Mangano, M</au><au>Zachwieja, S.J</au><au>Gordon-Kamm, W</au><au>Daines, R</au><au>Willetts, N.G</au><au>Chambers, S.A</au><au>Adam, W. Jr</au><au>Anderson, A</au><au>Williams, G</au><au>Haines, G. (DeKalb Genetics Corp., Mystic, Connecticut (USA))</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of microprojectile bombardment on embryogenic suspension cell cultures of maize (Zea mays L.) used for genetic transformation</atitle><jtitle>Planta</jtitle><date>1995</date><risdate>1995</risdate><volume>196</volume><issue>3</issue><spage>501</spage><epage>509</epage><pages>501-509</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>We have investigated the interaction between tungsten and gold microprojectiles with suspension-culture cells of maize used for genetic transformation. Particle size measurements were evaluated before and after DNA precipitation to determine mean particle size and the effect of DNA precipitation on particle aggregation. Following particle bombardment, metal foils were examined by scanning electron microscopy to visualize dispersion of individual particles and aggregates. Particle penetration into suspension-culture cell clusters was examined in paraffin-embedded bombarded cells serially sectioned and viewed with light microscopy and by energy dispersive X-ray microanalysis. Acridine-orange-stained bombarded cells were examined to observe cellular response to particle penetration. Transient expression of reporter genes C1 and B and GUS, (β-glucuronidase) were used to assess effects of particle bombardment on embryogenic cell types. Autoradiographic analysis of the transformable suspension cell culture SC82 (see Gordon-Kamm et al. 1990, Plant Cell 2, 603—618) was conducted to evaluate the S-phase and mitotic indices in embryogenic and nonembryogenic cells throughout a subculture passage and in response to DNA/particle delivery. The results of these investigations are discussed relative to cytodifferentiation of suspension cell clusters and recovery of transformed clonal sectors.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><doi>10.1007/BF00203650</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 1995, Vol.196 (3), p.501-509 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_crossref_primary_10_1007_BF00203650 |
| source | Jstor Complete Legacy; SpringerLink Journals - AutoHoldings |
| subjects | Agronomy. Soil science and plant productions Biolistics Biological and medical sciences Biotechnology CELL CULTURE Cell culture techniques CELL DIFFERENTIATION Cell lines Chemical suspensions Corn CULTIVO DE CELULAS CULTURE DE CELLULE Cultured cells DIFERENCIACION CELULAR DIFFERENCIATION CELLULAIRE DNA Embryonic cells Fundamental and applied biological sciences. Psychology Genetic engineering applications GENETIC TRANSFORMATION Genetics and breeding of economic plants Genuebertragung Particle size classes Pflanzenbau Pflanzenzuechtung Plant breeding: fundamental aspects and methodology Plasmid PLASMIDE PLASMIDIOS PLASMIDS TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Tungsten Zea ZEA MAYS Zelldifferenzierung Zellkultur |
| title | Effects of microprojectile bombardment on embryogenic suspension cell cultures of maize (Zea mays L.) used for genetic transformation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T10%3A03%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20microprojectile%20bombardment%20on%20embryogenic%20suspension%20cell%20cultures%20of%20maize%20(Zea%20mays%20L.)%20used%20for%20genetic%20transformation&rft.jtitle=Planta&rft.au=Kausch,%20A.P&rft.date=1995&rft.volume=196&rft.issue=3&rft.spage=501&rft.epage=509&rft.pages=501-509&rft.issn=0032-0935&rft.eissn=1432-2048&rft.coden=PLANAB&rft_id=info:doi/10.1007/BF00203650&rft_dat=%3Cjstor_cross%3E23383431%3C/jstor_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_jstor_id=23383431&rfr_iscdi=true |