Purification and characterization of fructokinase from developing tomato (Lycopersicon esculentum Mill.) fruits

A procedure is described which allows the purification of fructokinase (EC 2.7.1.4) from young tomato fruit. The procedure yielded a 400-fold purification and two isoenzymes designated fructokinase I and II (FKI and FKII) were separated by anion-exchange chromatography. Using sodium dodecyl sulfate-...

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Veröffentlicht in:	Planta 1996, Vol.199 (3), p.451-458
Hauptverfasser:	Martinez-Barajas, E, Randall, D.D. (Missouri Univ., Columbia (USA). Biochemistry Dept.)
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ADENOSINE TRIPHOSPHATE ADENOSINTRIFOSFATO Biological and medical sciences Corn ENZIMAS ENZYME ENZYMES ENZYMIC ACTIVITY ETAPAS DE DESARROLLO DE LA PLANTA Fructokinase FRUCTOSA FRUCTOSE FRUIT Fruits FRUTO Fundamental and applied biological sciences. Psychology Gels Gemuesebau ISOENZIMAS ISOENZYME ISOENZYMES LYCOPERSICON ESCULENTUM METABOLISM METABOLISME METABOLISMO Peas Pflanzenphysiologie Phosphorylation Physiological regulation PLANT DEVELOPMENTAL STAGES Plant physiology and development Plants Protein isoforms SACCHAROSE STADE DE DEVELOPPEMENT VEGETAL SUCROSA SUCROSE Tubers
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