Corneal endothelial wound repair in normal and mitotically inhibited cultures
The aim of the present study was to compare the morphology, proliferative activity and cytoskeletal organization of bovine corneal endothelial cells during wound healing under normal and mitotically inhibited conditions. Cell cultures were grown to confluency and incubated with the mitotic inhibitor...
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| Veröffentlicht in: | Graefe's archive for clinical and experimental ophthalmology 1995-11, Vol.233 (11), p.727-736 |
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| creator | MOHAY, J MCLAUGHLIN, B. J |
| description | The aim of the present study was to compare the morphology, proliferative activity and cytoskeletal organization of bovine corneal endothelial cells during wound healing under normal and mitotically inhibited conditions.
Cell cultures were grown to confluency and incubated with the mitotic inhibitor 5-fluorouracil (5-FU; 2.5 micrograms/ml) followed by a touch wound. Control cultures were maintained without 5-FU. Mitotic activity, F-actin, vinculin, vimentin and connexin 43 localization were evaluated before, during and after wound closure.
5-FU inhibited irreversibly the mitotic activity of corneal endothelial cells during the whole wound healing process. In the presence of 5-FU, a high degree of polymegathism and delay in actin and vinculin redistribution to the cell borders after wound closure was observed. Vimentin and connexin 43 immunolabeling revealed only slight differences between 5-FU-treated and control cultures.
Significant changes in cell geometry and cytoskeletal organization in the amitotic corneal endothelium became manifested only after wounding. These changes may influence cell-cell and cell-matrix interactions as well as functional restoration of the monolayer after wound closure. |
| doi_str_mv | 10.1007/BF00164678 |
| format | Article |
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Cell cultures were grown to confluency and incubated with the mitotic inhibitor 5-fluorouracil (5-FU; 2.5 micrograms/ml) followed by a touch wound. Control cultures were maintained without 5-FU. Mitotic activity, F-actin, vinculin, vimentin and connexin 43 localization were evaluated before, during and after wound closure.
5-FU inhibited irreversibly the mitotic activity of corneal endothelial cells during the whole wound healing process. In the presence of 5-FU, a high degree of polymegathism and delay in actin and vinculin redistribution to the cell borders after wound closure was observed. Vimentin and connexin 43 immunolabeling revealed only slight differences between 5-FU-treated and control cultures.
Significant changes in cell geometry and cytoskeletal organization in the amitotic corneal endothelium became manifested only after wounding. These changes may influence cell-cell and cell-matrix interactions as well as functional restoration of the monolayer after wound closure.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/BF00164678</identifier><identifier>PMID: 8566832</identifier><identifier>CODEN: GACODL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Antimetabolites - pharmacology ; Biological and medical sciences ; Bromodeoxyuridine - metabolism ; Cattle ; Cell Division ; Cells, Cultured ; Connexin 43 - metabolism ; Cytoskeletal Proteins - metabolism ; Cytoskeleton - metabolism ; Cytoskeleton - pathology ; DNA - biosynthesis ; Endothelium, Corneal - drug effects ; Endothelium, Corneal - pathology ; Endothelium, Corneal - physiology ; Fluorescent Antibody Technique ; Fluorouracil - pharmacology ; Injuries of the orbit. Foreign bodies of the eye. Diseases due to physical agents ; Medical sciences ; Mitosis - drug effects ; Traumas. Diseases due to physical agents ; Wound Healing - drug effects</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 1995-11, Vol.233 (11), p.727-736</ispartof><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-104401df920bda29e4f4e0c5af419c5c0da412d74676ab7bf7effedad1e1950e3</citedby><cites>FETCH-LOGICAL-c311t-104401df920bda29e4f4e0c5af419c5c0da412d74676ab7bf7effedad1e1950e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3698319$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8566832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOHAY, J</creatorcontrib><creatorcontrib>MCLAUGHLIN, B. J</creatorcontrib><title>Corneal endothelial wound repair in normal and mitotically inhibited cultures</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>The aim of the present study was to compare the morphology, proliferative activity and cytoskeletal organization of bovine corneal endothelial cells during wound healing under normal and mitotically inhibited conditions.
Cell cultures were grown to confluency and incubated with the mitotic inhibitor 5-fluorouracil (5-FU; 2.5 micrograms/ml) followed by a touch wound. Control cultures were maintained without 5-FU. Mitotic activity, F-actin, vinculin, vimentin and connexin 43 localization were evaluated before, during and after wound closure.
5-FU inhibited irreversibly the mitotic activity of corneal endothelial cells during the whole wound healing process. In the presence of 5-FU, a high degree of polymegathism and delay in actin and vinculin redistribution to the cell borders after wound closure was observed. Vimentin and connexin 43 immunolabeling revealed only slight differences between 5-FU-treated and control cultures.
Significant changes in cell geometry and cytoskeletal organization in the amitotic corneal endothelium became manifested only after wounding. These changes may influence cell-cell and cell-matrix interactions as well as functional restoration of the monolayer after wound closure.</description><subject>Animals</subject><subject>Antimetabolites - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Bromodeoxyuridine - metabolism</subject><subject>Cattle</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Connexin 43 - metabolism</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Cytoskeleton - metabolism</subject><subject>Cytoskeleton - pathology</subject><subject>DNA - biosynthesis</subject><subject>Endothelium, Corneal - drug effects</subject><subject>Endothelium, Corneal - pathology</subject><subject>Endothelium, Corneal - physiology</subject><subject>Fluorescent Antibody Technique</subject><subject>Fluorouracil - pharmacology</subject><subject>Injuries of the orbit. Foreign bodies of the eye. Diseases due to physical agents</subject><subject>Medical sciences</subject><subject>Mitosis - drug effects</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Wound Healing - drug effects</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1Lw0AQhhdRaq1evAs5eBKiM8kmmxw1WBUqXhR6C5vdWbqSj7KbIP33rrTU0wzv8zAwL2PXCPcIIB6elgCY81wUJ2yOPM1iAcn6lM1BJBgXabI-ZxfefwNAgDhjsyLL85DP2Xs1uJ5kG1Gvh3FDrQ37zzD1OnK0ldZFto_6wXUhliHs7DiMVsm23QWysY0dSUdqasfJkb9kZ0a2nq4Oc8G-ls-f1Wu8-nh5qx5XsUoRxxiBc0BtygQaLZOSuOEEKpOGY6kyBVpyTLQIH-WyEY0RZAxpqZGwzIDSBbvb31Vu8N6RqbfOdtLtaoT6r5L6v5Ig3-zl7dR0pI_qoYPAbw9c-vCYcbJX1h-1NC-LFMv0Fzk3abo</recordid><startdate>19951101</startdate><enddate>19951101</enddate><creator>MOHAY, J</creator><creator>MCLAUGHLIN, B. J</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19951101</creationdate><title>Corneal endothelial wound repair in normal and mitotically inhibited cultures</title><author>MOHAY, J ; MCLAUGHLIN, B. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-104401df920bda29e4f4e0c5af419c5c0da412d74676ab7bf7effedad1e1950e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Antimetabolites - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Bromodeoxyuridine - metabolism</topic><topic>Cattle</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Connexin 43 - metabolism</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Cytoskeleton - metabolism</topic><topic>Cytoskeleton - pathology</topic><topic>DNA - biosynthesis</topic><topic>Endothelium, Corneal - drug effects</topic><topic>Endothelium, Corneal - pathology</topic><topic>Endothelium, Corneal - physiology</topic><topic>Fluorescent Antibody Technique</topic><topic>Fluorouracil - pharmacology</topic><topic>Injuries of the orbit. Foreign bodies of the eye. Diseases due to physical agents</topic><topic>Medical sciences</topic><topic>Mitosis - drug effects</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOHAY, J</creatorcontrib><creatorcontrib>MCLAUGHLIN, B. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOHAY, J</au><au>MCLAUGHLIN, B. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corneal endothelial wound repair in normal and mitotically inhibited cultures</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>1995-11-01</date><risdate>1995</risdate><volume>233</volume><issue>11</issue><spage>727</spage><epage>736</epage><pages>727-736</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><coden>GACODL</coden><abstract>The aim of the present study was to compare the morphology, proliferative activity and cytoskeletal organization of bovine corneal endothelial cells during wound healing under normal and mitotically inhibited conditions.
Cell cultures were grown to confluency and incubated with the mitotic inhibitor 5-fluorouracil (5-FU; 2.5 micrograms/ml) followed by a touch wound. Control cultures were maintained without 5-FU. Mitotic activity, F-actin, vinculin, vimentin and connexin 43 localization were evaluated before, during and after wound closure.
5-FU inhibited irreversibly the mitotic activity of corneal endothelial cells during the whole wound healing process. In the presence of 5-FU, a high degree of polymegathism and delay in actin and vinculin redistribution to the cell borders after wound closure was observed. Vimentin and connexin 43 immunolabeling revealed only slight differences between 5-FU-treated and control cultures.
Significant changes in cell geometry and cytoskeletal organization in the amitotic corneal endothelium became manifested only after wounding. These changes may influence cell-cell and cell-matrix interactions as well as functional restoration of the monolayer after wound closure.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>8566832</pmid><doi>10.1007/BF00164678</doi><tpages>10</tpages></addata></record> |
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| ispartof | Graefe's archive for clinical and experimental ophthalmology, 1995-11, Vol.233 (11), p.727-736 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Antimetabolites - pharmacology Biological and medical sciences Bromodeoxyuridine - metabolism Cattle Cell Division Cells, Cultured Connexin 43 - metabolism Cytoskeletal Proteins - metabolism Cytoskeleton - metabolism Cytoskeleton - pathology DNA - biosynthesis Endothelium, Corneal - drug effects Endothelium, Corneal - pathology Endothelium, Corneal - physiology Fluorescent Antibody Technique Fluorouracil - pharmacology Injuries of the orbit. Foreign bodies of the eye. Diseases due to physical agents Medical sciences Mitosis - drug effects Traumas. Diseases due to physical agents Wound Healing - drug effects |
| title | Corneal endothelial wound repair in normal and mitotically inhibited cultures |
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