ELISA IgG as Screening Test for the Research of True Prevalence of Human Hydatidosis in Badajoz (Spain)
IgG antibodies against. E. granulosus were investigated in order to determine the true prevalence of human echinococcosis in Badajoz, Spain. The seroepidemiological survey was carried out on 1540 subjects not suspected of suffering from hydatidosis, in order to add this morbidity rate to that alread...
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| Veröffentlicht in: | European journal of epidemiology 1988-12, Vol.4 (4), p.439-444 |
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| container_end_page | 444 |
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| container_title | European journal of epidemiology |
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| creator | M. C. González Roiz M. P. Carmona Plá Giraldo, C. Pérez M. T. Blanco Roca Dubón, M. Beltrán |
| description | IgG antibodies against. E. granulosus were investigated in order to determine the true prevalence of human echinococcosis in Badajoz, Spain. The seroepidemiological survey was carried out on 1540 subjects not suspected of suffering from hydatidosis, in order to add this morbidity rate to that already declared. Because of its high sensitivity and specificity, a standardized immunoenzymatic technique, ELISA was used, and the results subsequently compared using a classical technique for seroepidemiological surveys, namely indirect hemagglutination test (IHA). According to ELISA, 2.2% of the tested subjects were positive. This indicates a higher morbidity in our province than that declared. This test was found to be ideal for seroepidemiological surveys since it is reproducible, easily and rapidly conducted and safe. It requires economical reagents, a large number of samples can be processed simultaneously and it offers a totally objective reading. |
| doi_str_mv | 10.1007/BF00146395 |
| format | Article |
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C. González Roiz ; M. P. Carmona Plá ; Giraldo, C. Pérez ; M. T. Blanco Roca ; Dubón, M. Beltrán</creator><creatorcontrib>M. C. González Roiz ; M. P. Carmona Plá ; Giraldo, C. Pérez ; M. T. Blanco Roca ; Dubón, M. Beltrán</creatorcontrib><description>IgG antibodies against. E. granulosus were investigated in order to determine the true prevalence of human echinococcosis in Badajoz, Spain. The seroepidemiological survey was carried out on 1540 subjects not suspected of suffering from hydatidosis, in order to add this morbidity rate to that already declared. Because of its high sensitivity and specificity, a standardized immunoenzymatic technique, ELISA was used, and the results subsequently compared using a classical technique for seroepidemiological surveys, namely indirect hemagglutination test (IHA). According to ELISA, 2.2% of the tested subjects were positive. This indicates a higher morbidity in our province than that declared. This test was found to be ideal for seroepidemiological surveys since it is reproducible, easily and rapidly conducted and safe. 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C. González Roiz</creatorcontrib><creatorcontrib>M. P. Carmona Plá</creatorcontrib><creatorcontrib>Giraldo, C. Pérez</creatorcontrib><creatorcontrib>M. T. Blanco Roca</creatorcontrib><creatorcontrib>Dubón, M. Beltrán</creatorcontrib><title>ELISA IgG as Screening Test for the Research of True Prevalence of Human Hydatidosis in Badajoz (Spain)</title><title>European journal of epidemiology</title><description>IgG antibodies against. E. granulosus were investigated in order to determine the true prevalence of human echinococcosis in Badajoz, Spain. The seroepidemiological survey was carried out on 1540 subjects not suspected of suffering from hydatidosis, in order to add this morbidity rate to that already declared. Because of its high sensitivity and specificity, a standardized immunoenzymatic technique, ELISA was used, and the results subsequently compared using a classical technique for seroepidemiological surveys, namely indirect hemagglutination test (IHA). According to ELISA, 2.2% of the tested subjects were positive. This indicates a higher morbidity in our province than that declared. This test was found to be ideal for seroepidemiological surveys since it is reproducible, easily and rapidly conducted and safe. It requires economical reagents, a large number of samples can be processed simultaneously and it offers a totally objective reading.</description><subject>Antibodies</subject><subject>Antigens</subject><subject>Echinococcosis</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Epidemiology</subject><subject>Hemagglutination</subject><subject>Hydatids</subject><subject>Morbidity rates</subject><subject>Optical density</subject><subject>Parasitism</subject><issn>0393-2990</issn><issn>0392-2990</issn><issn>1573-7284</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNpFkE1LAzEURYMoWKsb1y6yVGH0JZlMMsu29AsKiq3rIcm8tFPamZJMhfrrbano6sLlcOEeQu4ZvDAA9dofAbA0E7m8IB0mlUgU1-kl6YDIRcLzHK7JTYxrANCQyw5ZDmfTeY9Ol2NqIp27gFhX9ZIuMLbUN4G2K6QfGNEEt6KNp4uwR_oe8MtssHZ4qib7ranp5FCatiqbWEVa1bRvSrNuvunjfGeq-umWXHmziXj3m13yORouBpNk9jaeDnqzxHEJbcKytLTSeMWhtF7lNtNWZpanVjKj0ClUTh7_mSxFW3Kmda41cG1Tjr60VnTJ83nXhSbGgL7YhWprwqFgUJwUFf-KjvDDGV7Htgl_pJCcCS7FD8DgYRc</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>M. C. González Roiz</creator><creator>M. P. Carmona Plá</creator><creator>Giraldo, C. Pérez</creator><creator>M. T. Blanco Roca</creator><creator>Dubón, M. Beltrán</creator><general>Kluwer Academic Publishers</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19881201</creationdate><title>ELISA IgG as Screening Test for the Research of True Prevalence of Human Hydatidosis in Badajoz (Spain)</title><author>M. C. González Roiz ; M. P. Carmona Plá ; Giraldo, C. Pérez ; M. T. Blanco Roca ; Dubón, M. 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The seroepidemiological survey was carried out on 1540 subjects not suspected of suffering from hydatidosis, in order to add this morbidity rate to that already declared. Because of its high sensitivity and specificity, a standardized immunoenzymatic technique, ELISA was used, and the results subsequently compared using a classical technique for seroepidemiological surveys, namely indirect hemagglutination test (IHA). According to ELISA, 2.2% of the tested subjects were positive. This indicates a higher morbidity in our province than that declared. This test was found to be ideal for seroepidemiological surveys since it is reproducible, easily and rapidly conducted and safe. It requires economical reagents, a large number of samples can be processed simultaneously and it offers a totally objective reading.</abstract><pub>Kluwer Academic Publishers</pub><doi>10.1007/BF00146395</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0393-2990 |
| ispartof | European journal of epidemiology, 1988-12, Vol.4 (4), p.439-444 |
| issn | 0393-2990 0392-2990 1573-7284 |
| language | eng |
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| source | Jstor Complete Legacy; Springer Nature - Complete Springer Journals |
| subjects | Antibodies Antigens Echinococcosis Enzyme linked immunosorbent assay Epidemiology Hemagglutination Hydatids Morbidity rates Optical density Parasitism |
| title | ELISA IgG as Screening Test for the Research of True Prevalence of Human Hydatidosis in Badajoz (Spain) |
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