The cytogenetic and hepatotoxic effects of dioxin on mouse liver cells
The cytogenetic and hepatotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on mouse liver cells were investigated. Male C57BL/6J strain mice, which have TCDD receptors, were given single intraperitoneal injections of 25, 37.5, 75 and 150 micrograms of TCDD/kg body weight or corn oil carri...
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| Veröffentlicht in: | Cell biology and toxicology 1988-03, Vol.4 (1), p.31-40 |
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| creator | Brooks, A L Jordan, S W Bose, K K Smith, J Allison, D C |
| description | The cytogenetic and hepatotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on mouse liver cells were investigated. Male C57BL/6J strain mice, which have TCDD receptors, were given single intraperitoneal injections of 25, 37.5, 75 and 150 micrograms of TCDD/kg body weight or corn oil carrier alone. Two-thirds hepatectomies were carried out at 1 or 7 days after injection and chromosomal aberrations and mitotic indexes of the regenerating hepatocytes were scored 54 hr after hepatectomy. Liver sections from additional intact mice were studied for TCDD-hepatotoxicity at 1, 7 and 30 days after injection. The three high doses of TCDD caused hepatotoxicity with necrosis of liver cells and focal architectural collapse by 30 days after injection. No evidence was obtained of an increase in the frequency of chromosomal structural aberrations at doses that allowed sufficient mitotic activity for cytogenetic evaluation. We conclude that TCDD is not a clastogen for mouse hepatocytes, although high doses cause marked hepatocellular necrosis. |
| doi_str_mv | 10.1007/BF00141285 |
| format | Article |
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Male C57BL/6J strain mice, which have TCDD receptors, were given single intraperitoneal injections of 25, 37.5, 75 and 150 micrograms of TCDD/kg body weight or corn oil carrier alone. Two-thirds hepatectomies were carried out at 1 or 7 days after injection and chromosomal aberrations and mitotic indexes of the regenerating hepatocytes were scored 54 hr after hepatectomy. Liver sections from additional intact mice were studied for TCDD-hepatotoxicity at 1, 7 and 30 days after injection. The three high doses of TCDD caused hepatotoxicity with necrosis of liver cells and focal architectural collapse by 30 days after injection. No evidence was obtained of an increase in the frequency of chromosomal structural aberrations at doses that allowed sufficient mitotic activity for cytogenetic evaluation. We conclude that TCDD is not a clastogen for mouse hepatocytes, although high doses cause marked hepatocellular necrosis.</description><identifier>ISSN: 0742-2091</identifier><identifier>EISSN: 1573-6822</identifier><identifier>DOI: 10.1007/BF00141285</identifier><identifier>PMID: 2465810</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Cell Survival - drug effects ; Chromosome Aberrations ; Dioxins - toxicity ; Liver - cytology ; Liver - drug effects ; Liver - metabolism ; Mice ; Mice, Inbred Strains ; Mitotic Index ; Polychlorinated Dibenzodioxins - toxicity ; Staining and Labeling</subject><ispartof>Cell biology and toxicology, 1988-03, Vol.4 (1), p.31-40</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c282t-244cbbe1990615290bb3cd72534031037d026ce0f65e0928edf6ed353c6a72083</citedby><cites>FETCH-LOGICAL-c282t-244cbbe1990615290bb3cd72534031037d026ce0f65e0928edf6ed353c6a72083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2465810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brooks, A L</creatorcontrib><creatorcontrib>Jordan, S W</creatorcontrib><creatorcontrib>Bose, K K</creatorcontrib><creatorcontrib>Smith, J</creatorcontrib><creatorcontrib>Allison, D C</creatorcontrib><title>The cytogenetic and hepatotoxic effects of dioxin on mouse liver cells</title><title>Cell biology and toxicology</title><addtitle>Cell Biol Toxicol</addtitle><description>The cytogenetic and hepatotoxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on mouse liver cells were investigated. 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| ispartof | Cell biology and toxicology, 1988-03, Vol.4 (1), p.31-40 |
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| language | eng |
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| subjects | Animals Cell Survival - drug effects Chromosome Aberrations Dioxins - toxicity Liver - cytology Liver - drug effects Liver - metabolism Mice Mice, Inbred Strains Mitotic Index Polychlorinated Dibenzodioxins - toxicity Staining and Labeling |
| title | The cytogenetic and hepatotoxic effects of dioxin on mouse liver cells |
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