Differential response of Cu,Zn superoxide dismutases in two pea cultivars during a short-term exposure to sulfur dioxide
Pea cultivars Progress and Nugget have been shown previously to be differentially sensitive with respect to apparent photosynthesis in a short-term exposure to 0.8 microliters/l SO2. One possible contributing factor to the relative insensitivity of apparent photosynthesis of Progress to SO2 is an in...
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| Veröffentlicht in: | Plant molecular biology 1994-10, Vol.26 (1), p.95-103 |
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| creator | Madamanchi, N.R. (Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA). Dept. of Plant Pathology, Physiology and Weed Science) Donahue, J.L Cramer, C.L Alscher, R.G Pedersen, K |
| description | Pea cultivars Progress and Nugget have been shown previously to be differentially sensitive with respect to apparent photosynthesis in a short-term exposure to 0.8 microliters/l SO2. One possible contributing factor to the relative insensitivity of apparent photosynthesis of Progress to SO2 is an increase in superoxide dismutase (SOD) activities. We show here that both chloroplastic and cytoplastic Cu,Zn-SOD proteins increased in Progress on exposure to sulfur dioxide whereas both proteins decreased in Nugget. The increase in cytosolic Cu,Zn-SOD protein was greater than that of chloroplastic Cu,Zn-SOD protein. Using a gene-specific probe for the plastid SOD, northern blot analysis revealed an initial decrease in transcript abundance of the chloroplastic Cu,Zn-SOD gene in Progress on exposure to SO2 with an eventual recovery to pre-exposure levels. The transcript levels of the chloroplastic Cu,Zn-SOD decreased in Nugget over the time period of the exposure. These results suggest that a combination of translational and post-translational mechanisms may be involved in SO2-induced changes in cytosolic and plastidic Cu,Zn-SODs in pea. |
| doi_str_mv | 10.1007/BF00039523 |
| format | Article |
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(Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA). Dept. of Plant Pathology, Physiology and Weed Science) ; Donahue, J.L ; Cramer, C.L ; Alscher, R.G ; Pedersen, K</creator><creatorcontrib>Madamanchi, N.R. (Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA). Dept. of Plant Pathology, Physiology and Weed Science) ; Donahue, J.L ; Cramer, C.L ; Alscher, R.G ; Pedersen, K</creatorcontrib><description>Pea cultivars Progress and Nugget have been shown previously to be differentially sensitive with respect to apparent photosynthesis in a short-term exposure to 0.8 microliters/l SO2. One possible contributing factor to the relative insensitivity of apparent photosynthesis of Progress to SO2 is an increase in superoxide dismutase (SOD) activities. We show here that both chloroplastic and cytoplastic Cu,Zn-SOD proteins increased in Progress on exposure to sulfur dioxide whereas both proteins decreased in Nugget. The increase in cytosolic Cu,Zn-SOD protein was greater than that of chloroplastic Cu,Zn-SOD protein. Using a gene-specific probe for the plastid SOD, northern blot analysis revealed an initial decrease in transcript abundance of the chloroplastic Cu,Zn-SOD gene in Progress on exposure to SO2 with an eventual recovery to pre-exposure levels. The transcript levels of the chloroplastic Cu,Zn-SOD decreased in Nugget over the time period of the exposure. 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(Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA). Dept. of Plant Pathology, Physiology and Weed Science)</creatorcontrib><creatorcontrib>Donahue, J.L</creatorcontrib><creatorcontrib>Cramer, C.L</creatorcontrib><creatorcontrib>Alscher, R.G</creatorcontrib><creatorcontrib>Pedersen, K</creatorcontrib><title>Differential response of Cu,Zn superoxide dismutases in two pea cultivars during a short-term exposure to sulfur dioxide</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Pea cultivars Progress and Nugget have been shown previously to be differentially sensitive with respect to apparent photosynthesis in a short-term exposure to 0.8 microliters/l SO2. One possible contributing factor to the relative insensitivity of apparent photosynthesis of Progress to SO2 is an increase in superoxide dismutase (SOD) activities. We show here that both chloroplastic and cytoplastic Cu,Zn-SOD proteins increased in Progress on exposure to sulfur dioxide whereas both proteins decreased in Nugget. The increase in cytosolic Cu,Zn-SOD protein was greater than that of chloroplastic Cu,Zn-SOD protein. Using a gene-specific probe for the plastid SOD, northern blot analysis revealed an initial decrease in transcript abundance of the chloroplastic Cu,Zn-SOD gene in Progress on exposure to SO2 with an eventual recovery to pre-exposure levels. The transcript levels of the chloroplastic Cu,Zn-SOD decreased in Nugget over the time period of the exposure. These results suggest that a combination of translational and post-translational mechanisms may be involved in SO2-induced changes in cytosolic and plastidic Cu,Zn-SODs in pea.</description><subject>AIR POLLUTION</subject><subject>CHLOROPLASTE</subject><subject>CHLOROPLASTS</subject><subject>Chloroplasts - chemistry</subject><subject>Chloroplasts - metabolism</subject><subject>CINC</subject><subject>CLOROPLASTO</subject><subject>COBRE</subject><subject>COPPER</subject><subject>CUIVRE</subject><subject>Cytoplasm - chemistry</subject><subject>Cytoplasm - metabolism</subject><subject>DIOXIDO DE AZUFRE</subject><subject>DIOXYDE DE SOUFRE</subject><subject>DNA, Plant - analysis</subject><subject>ESTRES</subject><subject>FOTOSINTESIS</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>PHOTOSYNTHESE</subject><subject>PHOTOSYNTHESIS</subject><subject>PISUM SATIVUM</subject><subject>Pisum sativum - enzymology</subject><subject>Pisum sativum - genetics</subject><subject>POLLUTION ATMOSPHERIQUE</subject><subject>POLUCION DEL AIRE</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Plant - analysis</subject><subject>STRESS</subject><subject>Sulfur Dioxide - pharmacology</subject><subject>SULPHUR DIOXIDE</subject><subject>SUPEROXIDE DISMUTASE</subject><subject>Superoxide Dismutase - biosynthesis</subject><subject>Superoxide Dismutase - genetics</subject><subject>SUPEROXIDO DISMUTASA</subject><subject>SUPEROXYDE DISMUTASE</subject><subject>Transcription, Genetic - drug effects</subject><subject>VARIEDADES</subject><subject>VARIETE</subject><subject>VARIETIES</subject><subject>ZINC</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAURS0EKqWwMCIheUYEnmPnwyMUCkgVLLCwRE7yXIySOLIdKP-eQCuY7nCPznAIOWZwwQCyy-sFAHCZxHyHTFmS8SiBON8lU2BpFgnB4n1y4P07wIjzdEImmRS5BDkl6xujNTrsglENdeh723mkVtP5cP7aUT_06Oza1Ehr49shKI-emo6GT0t7VLQammA-lPO0HpzpVlRR_2ZdiAK6luK6t35wSIMdVY0e3Kj51R2SPa0aj0fbnZGXxe3z_D5aPt09zK-WURWzNESaMcwVg0qwkiFUZZ6IjGNaQqqYTlOWVglntaiBCw1K1ULkcS1lXvIqRxnzGTnbeCtnvXeoi96ZVrmvgkHxU6_4rzfCpxu4H8oW6z90m2v8Tza_VrZQK2d88biUyZg5lvwbvB9z4A</recordid><startdate>199410</startdate><enddate>199410</enddate><creator>Madamanchi, N.R. 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Dept. of Plant Pathology, Physiology and Weed Science) ; Donahue, J.L ; Cramer, C.L ; Alscher, R.G ; Pedersen, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c216t-f11e8a10c41b1e0cb85473e6b06a1f6616c531d4d034f0aad4482d998b3c8e923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>AIR POLLUTION</topic><topic>CHLOROPLASTE</topic><topic>CHLOROPLASTS</topic><topic>Chloroplasts - chemistry</topic><topic>Chloroplasts - metabolism</topic><topic>CINC</topic><topic>CLOROPLASTO</topic><topic>COBRE</topic><topic>COPPER</topic><topic>CUIVRE</topic><topic>Cytoplasm - chemistry</topic><topic>Cytoplasm - metabolism</topic><topic>DIOXIDO DE AZUFRE</topic><topic>DIOXYDE DE SOUFRE</topic><topic>DNA, Plant - analysis</topic><topic>ESTRES</topic><topic>FOTOSINTESIS</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>PHOTOSYNTHESE</topic><topic>PHOTOSYNTHESIS</topic><topic>PISUM SATIVUM</topic><topic>Pisum sativum - enzymology</topic><topic>Pisum sativum - genetics</topic><topic>POLLUTION ATMOSPHERIQUE</topic><topic>POLUCION DEL AIRE</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Plant - analysis</topic><topic>STRESS</topic><topic>Sulfur Dioxide - pharmacology</topic><topic>SULPHUR DIOXIDE</topic><topic>SUPEROXIDE DISMUTASE</topic><topic>Superoxide Dismutase - biosynthesis</topic><topic>Superoxide Dismutase - genetics</topic><topic>SUPEROXIDO DISMUTASA</topic><topic>SUPEROXYDE DISMUTASE</topic><topic>Transcription, Genetic - drug effects</topic><topic>VARIEDADES</topic><topic>VARIETE</topic><topic>VARIETIES</topic><topic>ZINC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Madamanchi, N.R. (Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA). Dept. of Plant Pathology, Physiology and Weed Science)</creatorcontrib><creatorcontrib>Donahue, J.L</creatorcontrib><creatorcontrib>Cramer, C.L</creatorcontrib><creatorcontrib>Alscher, R.G</creatorcontrib><creatorcontrib>Pedersen, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Madamanchi, N.R. (Virginia Polytechnic Inst. and State Univ., Blacksburg, VA (USA). Dept. of Plant Pathology, Physiology and Weed Science)</au><au>Donahue, J.L</au><au>Cramer, C.L</au><au>Alscher, R.G</au><au>Pedersen, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential response of Cu,Zn superoxide dismutases in two pea cultivars during a short-term exposure to sulfur dioxide</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1994-10</date><risdate>1994</risdate><volume>26</volume><issue>1</issue><spage>95</spage><epage>103</epage><pages>95-103</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Pea cultivars Progress and Nugget have been shown previously to be differentially sensitive with respect to apparent photosynthesis in a short-term exposure to 0.8 microliters/l SO2. One possible contributing factor to the relative insensitivity of apparent photosynthesis of Progress to SO2 is an increase in superoxide dismutase (SOD) activities. We show here that both chloroplastic and cytoplastic Cu,Zn-SOD proteins increased in Progress on exposure to sulfur dioxide whereas both proteins decreased in Nugget. The increase in cytosolic Cu,Zn-SOD protein was greater than that of chloroplastic Cu,Zn-SOD protein. Using a gene-specific probe for the plastid SOD, northern blot analysis revealed an initial decrease in transcript abundance of the chloroplastic Cu,Zn-SOD gene in Progress on exposure to SO2 with an eventual recovery to pre-exposure levels. The transcript levels of the chloroplastic Cu,Zn-SOD decreased in Nugget over the time period of the exposure. These results suggest that a combination of translational and post-translational mechanisms may be involved in SO2-induced changes in cytosolic and plastidic Cu,Zn-SODs in pea.</abstract><cop>Netherlands</cop><pmid>7948909</pmid><doi>10.1007/BF00039523</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant molecular biology, 1994-10, Vol.26 (1), p.95-103 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | AIR POLLUTION CHLOROPLASTE CHLOROPLASTS Chloroplasts - chemistry Chloroplasts - metabolism CINC CLOROPLASTO COBRE COPPER CUIVRE Cytoplasm - chemistry Cytoplasm - metabolism DIOXIDO DE AZUFRE DIOXYDE DE SOUFRE DNA, Plant - analysis ESTRES FOTOSINTESIS Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Plant - drug effects PHOTOSYNTHESE PHOTOSYNTHESIS PISUM SATIVUM Pisum sativum - enzymology Pisum sativum - genetics POLLUTION ATMOSPHERIQUE POLUCION DEL AIRE RNA, Messenger - analysis RNA, Plant - analysis STRESS Sulfur Dioxide - pharmacology SULPHUR DIOXIDE SUPEROXIDE DISMUTASE Superoxide Dismutase - biosynthesis Superoxide Dismutase - genetics SUPEROXIDO DISMUTASA SUPEROXYDE DISMUTASE Transcription, Genetic - drug effects VARIEDADES VARIETE VARIETIES ZINC |
| title | Differential response of Cu,Zn superoxide dismutases in two pea cultivars during a short-term exposure to sulfur dioxide |
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