Purification of Sepharose-Unbinding Ricin from Castor Beans (Ricinus communis) by Hydroxyapatite Chromatography

Sepharose-unbinding ricin was one-step separated and purified from a crude extract of castor beans (Ricinus communis) by affinity chromatography on hydroxyapatite. This purification method does not require the time-consuming and complicated steps, such as gel filtration and ion-exchange chromatograp...

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Veröffentlicht in:	Protein expression and purification 1998-07, Vol.13 (2), p.150-154
Hauptverfasser:	Woo, Byung Ho, Lee, Jung Tae, Lee, Kang Choon
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Affinity - methods Durapatite Electrophoresis, Polyacrylamide Gel Isoelectric Focusing Lethal Dose 50 Plant Lectins Plants, Toxic Ricin - isolation & purification Ricin - toxicity Ricinus communis - chemistry Seeds - chemistry
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