Production, Purification, and Characterization of Recombinant Human Hemoglobin Rainier Expressed inSaccharomyces cerevisiae

Hemoglobin Rainier is a naturally occurring hemoglobin variant in which the β145 tyrosine is substituted with cysteine. The α and βRainierglobin cDNAs were cloned in a high copy number vector and expressed inSaccharomyces cerevisiaeunder the control of galactose-regulated hybrid promoters. Using thi...

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Veröffentlicht in:Protein expression and purification 1996-12, Vol.8 (4), p.447-455
Hauptverfasser: Motwani, Nalini, Talarico, Todd, Jain, Sanjay, Bajwa, Wajeeh, Blackburn, Robert, Nwosu, Veronica, Holland, Michael, Deangelo, Joseph, Privalle, Christopher, Keng, Teresa
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Sprache:eng
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