Comparison of the large subunit ribosomal DNA ofNeosporaandToxoplasmaand development of a new genetic marker for their differentiation based on the D2 domain

Comparison of the large subunit ribosomal DNA ofNeosporaandToxoplasmaand development of a new genetic marker for their differentiation based on the D2 domain

The latest release of the large subunit ribosomal database contains 429 sequences, yet only 10 (six nuclear and four mitochondrial) are derived from parasites of the phylum Apicomplexa. Three of these (allToxoplasma gondii) were previously contained in the 1994 release of the database. As an initiat...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular and cellular probes 1998-02, Vol.12 (1), p.1-13
Hauptverfasser: Ellis, J.T, Amoyal, G, Ryce, C, Harper, P.A.W, Clough, K.A, Homan, W.L, Brindley, P.J
Format: Artikel
Sprache:eng
Schlagworte:
Neospora,Toxoplasma, large subunit ribosomal DNA, secondary structure, polymerase chain reaction
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 13
container_issue 1
container_start_page 1
container_title Molecular and cellular probes
container_volume 12
creator Ellis, J.T
Amoyal, G
Ryce, C
Harper, P.A.W
Clough, K.A
Homan, W.L
Brindley, P.J
description The latest release of the large subunit ribosomal database contains 429 sequences, yet only 10 (six nuclear and four mitochondrial) are derived from parasites of the phylum Apicomplexa. Three of these (allToxoplasma gondii) were previously contained in the 1994 release of the database. As an initiative towards an understanding of ribosomal gene organization in the Apicomplexa, the primary sequence of the large subunit (LSU) rDNA ofNeospora caninumis presented, and compared with a consensus sequence derived for the LSU rDNA ofT. gondii. Nucleotide differences observed between these two taxa in the D2 expansion segment (or domain) (also called the C1/C1′ region) of the LSU rDNA were incorporated into a primer that forms the basis of a species-specific polymerase chain reaction (PCR) forN. caninum. The D2 domain of the LSU rDNA, therefore, represents a new genetic marker that can be used for the differentiation and identification ofNeosporafrom other cyst-forming coccidia.
doi_str_mv 10.1006/mcpr.1997.0143
format Article
fullrecord <record><control><sourceid>elsevier_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1006_mcpr_1997_0143</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0890850897901432</els_id><sourcerecordid>S0890850897901432</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1333-333a1217da2d334ad25ee5a040157b6f6c341c0054ee9d3d04b089ea7e511e9b3</originalsourceid><addsrcrecordid>eNp1kE1OwzAQhS0EEqWwZe0LJIxjJ02WVcufVJVNWVuOPSmGJI7stMBhuCuOypbFaGakefOePkJuGaQMoLjr9OBTVlWLFJjgZ2TGoCoSxipxTmZQVpCUOZSX5CqEdwCoBJQz8rNy3aC8Da6nrqHjG9JW-T3ScKgPvR2pt7ULrlMtXW-X8WSLLgzOK9WbnftyQ6tCNy3U4BFbN3TYj9MnRXv8pHvscbSadsp_oKeN85OF9dTYpkEfb60abfSuVUBD4zAlWGfUREvbX5OLRrUBb_76nLw-3O9WT8nm5fF5tdwkmnHOk1iKZWxhVGY4F8pkOWKuQADLF3XRFJoLpgFygVgZbkDUkQeqBeaMYVXzOUlPf7V3IXhs5OBtzPwtGcgJrpzgygmunOBGQXkSYEx1tOhl0BZ7jcZ61KM0zv4n_QUnSIQT</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Comparison of the large subunit ribosomal DNA ofNeosporaandToxoplasmaand development of a new genetic marker for their differentiation based on the D2 domain</title><source>Access via ScienceDirect (Elsevier)</source><creator>Ellis, J.T ; Amoyal, G ; Ryce, C ; Harper, P.A.W ; Clough, K.A ; Homan, W.L ; Brindley, P.J</creator><creatorcontrib>Ellis, J.T ; Amoyal, G ; Ryce, C ; Harper, P.A.W ; Clough, K.A ; Homan, W.L ; Brindley, P.J</creatorcontrib><description>The latest release of the large subunit ribosomal database contains 429 sequences, yet only 10 (six nuclear and four mitochondrial) are derived from parasites of the phylum Apicomplexa. Three of these (allToxoplasma gondii) were previously contained in the 1994 release of the database. As an initiative towards an understanding of ribosomal gene organization in the Apicomplexa, the primary sequence of the large subunit (LSU) rDNA ofNeospora caninumis presented, and compared with a consensus sequence derived for the LSU rDNA ofT. gondii. Nucleotide differences observed between these two taxa in the D2 expansion segment (or domain) (also called the C1/C1′ region) of the LSU rDNA were incorporated into a primer that forms the basis of a species-specific polymerase chain reaction (PCR) forN. caninum. The D2 domain of the LSU rDNA, therefore, represents a new genetic marker that can be used for the differentiation and identification ofNeosporafrom other cyst-forming coccidia.</description><identifier>ISSN: 0890-8508</identifier><identifier>EISSN: 1096-1194</identifier><identifier>DOI: 10.1006/mcpr.1997.0143</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Neospora,Toxoplasma, large subunit ribosomal DNA, secondary structure, polymerase chain reaction</subject><ispartof>Molecular and cellular probes, 1998-02, Vol.12 (1), p.1-13</ispartof><rights>1998 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1333-333a1217da2d334ad25ee5a040157b6f6c341c0054ee9d3d04b089ea7e511e9b3</citedby><cites>FETCH-LOGICAL-c1333-333a1217da2d334ad25ee5a040157b6f6c341c0054ee9d3d04b089ea7e511e9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mcpr.1997.0143$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Ellis, J.T</creatorcontrib><creatorcontrib>Amoyal, G</creatorcontrib><creatorcontrib>Ryce, C</creatorcontrib><creatorcontrib>Harper, P.A.W</creatorcontrib><creatorcontrib>Clough, K.A</creatorcontrib><creatorcontrib>Homan, W.L</creatorcontrib><creatorcontrib>Brindley, P.J</creatorcontrib><title>Comparison of the large subunit ribosomal DNA ofNeosporaandToxoplasmaand development of a new genetic marker for their differentiation based on the D2 domain</title><title>Molecular and cellular probes</title><description>The latest release of the large subunit ribosomal database contains 429 sequences, yet only 10 (six nuclear and four mitochondrial) are derived from parasites of the phylum Apicomplexa. Three of these (allToxoplasma gondii) were previously contained in the 1994 release of the database. As an initiative towards an understanding of ribosomal gene organization in the Apicomplexa, the primary sequence of the large subunit (LSU) rDNA ofNeospora caninumis presented, and compared with a consensus sequence derived for the LSU rDNA ofT. gondii. Nucleotide differences observed between these two taxa in the D2 expansion segment (or domain) (also called the C1/C1′ region) of the LSU rDNA were incorporated into a primer that forms the basis of a species-specific polymerase chain reaction (PCR) forN. caninum. The D2 domain of the LSU rDNA, therefore, represents a new genetic marker that can be used for the differentiation and identification ofNeosporafrom other cyst-forming coccidia.</description><subject>Neospora,Toxoplasma, large subunit ribosomal DNA, secondary structure, polymerase chain reaction</subject><issn>0890-8508</issn><issn>1096-1194</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNp1kE1OwzAQhS0EEqWwZe0LJIxjJ02WVcufVJVNWVuOPSmGJI7stMBhuCuOypbFaGakefOePkJuGaQMoLjr9OBTVlWLFJjgZ2TGoCoSxipxTmZQVpCUOZSX5CqEdwCoBJQz8rNy3aC8Da6nrqHjG9JW-T3ScKgPvR2pt7ULrlMtXW-X8WSLLgzOK9WbnftyQ6tCNy3U4BFbN3TYj9MnRXv8pHvscbSadsp_oKeN85OF9dTYpkEfb60abfSuVUBD4zAlWGfUREvbX5OLRrUBb_76nLw-3O9WT8nm5fF5tdwkmnHOk1iKZWxhVGY4F8pkOWKuQADLF3XRFJoLpgFygVgZbkDUkQeqBeaMYVXzOUlPf7V3IXhs5OBtzPwtGcgJrpzgygmunOBGQXkSYEx1tOhl0BZ7jcZ61KM0zv4n_QUnSIQT</recordid><startdate>199802</startdate><enddate>199802</enddate><creator>Ellis, J.T</creator><creator>Amoyal, G</creator><creator>Ryce, C</creator><creator>Harper, P.A.W</creator><creator>Clough, K.A</creator><creator>Homan, W.L</creator><creator>Brindley, P.J</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199802</creationdate><title>Comparison of the large subunit ribosomal DNA ofNeosporaandToxoplasmaand development of a new genetic marker for their differentiation based on the D2 domain</title><author>Ellis, J.T ; Amoyal, G ; Ryce, C ; Harper, P.A.W ; Clough, K.A ; Homan, W.L ; Brindley, P.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1333-333a1217da2d334ad25ee5a040157b6f6c341c0054ee9d3d04b089ea7e511e9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Neospora,Toxoplasma, large subunit ribosomal DNA, secondary structure, polymerase chain reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ellis, J.T</creatorcontrib><creatorcontrib>Amoyal, G</creatorcontrib><creatorcontrib>Ryce, C</creatorcontrib><creatorcontrib>Harper, P.A.W</creatorcontrib><creatorcontrib>Clough, K.A</creatorcontrib><creatorcontrib>Homan, W.L</creatorcontrib><creatorcontrib>Brindley, P.J</creatorcontrib><collection>CrossRef</collection><jtitle>Molecular and cellular probes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ellis, J.T</au><au>Amoyal, G</au><au>Ryce, C</au><au>Harper, P.A.W</au><au>Clough, K.A</au><au>Homan, W.L</au><au>Brindley, P.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the large subunit ribosomal DNA ofNeosporaandToxoplasmaand development of a new genetic marker for their differentiation based on the D2 domain</atitle><jtitle>Molecular and cellular probes</jtitle><date>1998-02</date><risdate>1998</risdate><volume>12</volume><issue>1</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>0890-8508</issn><eissn>1096-1194</eissn><abstract>The latest release of the large subunit ribosomal database contains 429 sequences, yet only 10 (six nuclear and four mitochondrial) are derived from parasites of the phylum Apicomplexa. Three of these (allToxoplasma gondii) were previously contained in the 1994 release of the database. As an initiative towards an understanding of ribosomal gene organization in the Apicomplexa, the primary sequence of the large subunit (LSU) rDNA ofNeospora caninumis presented, and compared with a consensus sequence derived for the LSU rDNA ofT. gondii. Nucleotide differences observed between these two taxa in the D2 expansion segment (or domain) (also called the C1/C1′ region) of the LSU rDNA were incorporated into a primer that forms the basis of a species-specific polymerase chain reaction (PCR) forN. caninum. The D2 domain of the LSU rDNA, therefore, represents a new genetic marker that can be used for the differentiation and identification ofNeosporafrom other cyst-forming coccidia.</abstract><pub>Elsevier Ltd</pub><doi>10.1006/mcpr.1997.0143</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0890-8508
ispartof Molecular and cellular probes, 1998-02, Vol.12 (1), p.1-13
issn 0890-8508
1096-1194
language eng
recordid cdi_crossref_primary_10_1006_mcpr_1997_0143
source Access via ScienceDirect (Elsevier)
subjects Neospora,Toxoplasma, large subunit ribosomal DNA, secondary structure, polymerase chain reaction
title Comparison of the large subunit ribosomal DNA ofNeosporaandToxoplasmaand development of a new genetic marker for their differentiation based on the D2 domain
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T19%3A07%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-elsevier_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20the%20large%20subunit%20ribosomal%20DNA%20ofNeosporaandToxoplasmaand%20development%20of%20a%20new%20genetic%20marker%20for%20their%20differentiation%20based%20on%20the%20D2%20domain&rft.jtitle=Molecular%20and%20cellular%20probes&rft.au=Ellis,%20J.T&rft.date=1998-02&rft.volume=12&rft.issue=1&rft.spage=1&rft.epage=13&rft.pages=1-13&rft.issn=0890-8508&rft.eissn=1096-1194&rft_id=info:doi/10.1006/mcpr.1997.0143&rft_dat=%3Celsevier_cross%3ES0890850897901432%3C/elsevier_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_els_id=S0890850897901432&rfr_iscdi=true