Direct detection of Listeria monocytogenes using paramagnetic bead DNA extraction and enzymatic DNA amplification

The potential of the polymerase chain reaction (PCR) as a tool for direct detection of Listeria monoctogenes in food and clinical was investigated. A specific oligonucleotide, directed against the listeriolysin 0 gene of L. monocytogenes, was coupled to paramagnetic beads and used to isolate listeri...

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Veröffentlicht in:	Molecular and cellular probes 1994-06, Vol.8 (3), p.223-228
Hauptverfasser:	Niederhauser, C., Lüthy, J., Candrian, U.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA, Bacterial - analysis DNA, Bacterial - genetics Food Microbiology food, blood, Listeria monocytogenes, listeriolysin, PCR Gene Amplification Humans Immunomagnetic Separation Listeria monocytogenes - genetics Listeria monocytogenes - isolation & purification Methods Molecular Sequence Data Polymerase Chain Reaction Sensitivity and Specificity
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