Effect of Selector Coverage and Mobile Phase Composition on Enantiomeric Separations with Ristocetin A Chiral Stationary Phases
Three different amounts (i.e., selector coverages) of ristocetin A macrocyclic antibiotic have been covalently bonded to silica gel, packed in stainless steel columns, and evaluated as chiral stationary phases (CSPs) in high-performance liquid chromatography (HPLC). The three columns (i.e., CSPs) we...
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| Veröffentlicht in: | Microchemical journal 1999-05, Vol.62 (1), p.26-49 |
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| creator | Ekborg-Ott, K.Helen Wang, Xiande Armstrong, Daniel W. |
| description | Three different amounts (i.e., selector coverages) of ristocetin A macrocyclic antibiotic have been covalently bonded to silica gel, packed in stainless steel columns, and evaluated as chiral stationary phases (CSPs) in high-performance liquid chromatography (HPLC). The three columns (i.e., CSPs) were examined and compared in the reversed-phase, polar-organic, and normal-phase modes. The retention behavior (k′), selectivity (α), resolution (RS), and number of theoretical plates (N) for all three columns are discussed and compared in each mobile phase mode. The coverage of the chiral selector had a profound effect on the chromatographic behavior of the more retained enantiomers. A special mobile phase composition (i.e., methanol plus very small amounts of acid and base modifiers) resulted in improved baseline separations, without greatly affecting the analysis times, for all chiral analytes evaluated. The column stabilities were excellent when switching between different mobile phase modes. |
| doi_str_mv | 10.1006/mchj.1999.1730 |
| format | Article |
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The three columns (i.e., CSPs) were examined and compared in the reversed-phase, polar-organic, and normal-phase modes. The retention behavior (k′), selectivity (α), resolution (RS), and number of theoretical plates (N) for all three columns are discussed and compared in each mobile phase mode. The coverage of the chiral selector had a profound effect on the chromatographic behavior of the more retained enantiomers. A special mobile phase composition (i.e., methanol plus very small amounts of acid and base modifiers) resulted in improved baseline separations, without greatly affecting the analysis times, for all chiral analytes evaluated. The column stabilities were excellent when switching between different mobile phase modes.</description><identifier>ISSN: 0026-265X</identifier><identifier>EISSN: 1095-9149</identifier><identifier>DOI: 10.1006/mchj.1999.1730</identifier><identifier>CODEN: MICJAN</identifier><language>eng</language><publisher>New York, NY: Elsevier B.V</publisher><subject>amino acids ; Aminoacids, peptides. Hormones. Neuropeptides ; Analytical chemistry ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; enantiomeric separations ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; macrocyclic antibiotic ; Other chromatographic methods ; Proteins ; ristocetin A ; selector coverage</subject><ispartof>Microchemical journal, 1999-05, Vol.62 (1), p.26-49</ispartof><rights>1999 Academic Press</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c315t-cf0498b19a35eb77451f070c8f3d21acc01e741afeda404a4d7078b82f6fc23a3</citedby><cites>FETCH-LOGICAL-c315t-cf0498b19a35eb77451f070c8f3d21acc01e741afeda404a4d7078b82f6fc23a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0026265X99917307$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1793658$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Ekborg-Ott, K.Helen</creatorcontrib><creatorcontrib>Wang, Xiande</creatorcontrib><creatorcontrib>Armstrong, Daniel W.</creatorcontrib><title>Effect of Selector Coverage and Mobile Phase Composition on Enantiomeric Separations with Ristocetin A Chiral Stationary Phases</title><title>Microchemical journal</title><description>Three different amounts (i.e., selector coverages) of ristocetin A macrocyclic antibiotic have been covalently bonded to silica gel, packed in stainless steel columns, and evaluated as chiral stationary phases (CSPs) in high-performance liquid chromatography (HPLC). The three columns (i.e., CSPs) were examined and compared in the reversed-phase, polar-organic, and normal-phase modes. The retention behavior (k′), selectivity (α), resolution (RS), and number of theoretical plates (N) for all three columns are discussed and compared in each mobile phase mode. The coverage of the chiral selector had a profound effect on the chromatographic behavior of the more retained enantiomers. A special mobile phase composition (i.e., methanol plus very small amounts of acid and base modifiers) resulted in improved baseline separations, without greatly affecting the analysis times, for all chiral analytes evaluated. The column stabilities were excellent when switching between different mobile phase modes.</description><subject>amino acids</subject><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>enantiomeric separations</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>macrocyclic antibiotic</subject><subject>Other chromatographic methods</subject><subject>Proteins</subject><subject>ristocetin A</subject><subject>selector coverage</subject><issn>0026-265X</issn><issn>1095-9149</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNp1kM1LAzEQxYMoWKtXzzl43TrZr2yOpdQPqChWwdsyzU5synZTkqXiyX_drBU8CQMz8N6bGX6MXQqYCIDyeqvXm4lQSk2EzOCIjQSoIlEiV8dsBJCWSVoWb6fsLIQNAMgiFSP2NTeGdM-d4Utq4-Q8n7k9eXwnjl3DH9zKtsSf1hgoKtudC7a3ruOx5h12cd6StzrGd-hxkAL_sP2aP9vQO0297fiUz9bWY8uX_Y8D_edhYzhnJwbbQBe_fcxeb-Yvs7tk8Xh7P5suEp2Jok-0gVxVK6EwK2glZV4IAxJ0ZbImFag1CJK5QEMN5pBj3kiQ1apKTWl0mmE2ZpPDXu1dCJ5MvfN2G_-oBdQDvnrAVw_46gFfDFwdAjsMGlvjsdM2_KWkysqiirbqYKP4_N6Sr4O21GlqrI8068bZ_y58A4g6he4</recordid><startdate>19990501</startdate><enddate>19990501</enddate><creator>Ekborg-Ott, K.Helen</creator><creator>Wang, Xiande</creator><creator>Armstrong, Daniel W.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19990501</creationdate><title>Effect of Selector Coverage and Mobile Phase Composition on Enantiomeric Separations with Ristocetin A Chiral Stationary Phases</title><author>Ekborg-Ott, K.Helen ; Wang, Xiande ; Armstrong, Daniel W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c315t-cf0498b19a35eb77451f070c8f3d21acc01e741afeda404a4d7078b82f6fc23a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>amino acids</topic><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical chemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>enantiomeric separations</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>macrocyclic antibiotic</topic><topic>Other chromatographic methods</topic><topic>Proteins</topic><topic>ristocetin A</topic><topic>selector coverage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ekborg-Ott, K.Helen</creatorcontrib><creatorcontrib>Wang, Xiande</creatorcontrib><creatorcontrib>Armstrong, Daniel W.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Microchemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ekborg-Ott, K.Helen</au><au>Wang, Xiande</au><au>Armstrong, Daniel W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Selector Coverage and Mobile Phase Composition on Enantiomeric Separations with Ristocetin A Chiral Stationary Phases</atitle><jtitle>Microchemical journal</jtitle><date>1999-05-01</date><risdate>1999</risdate><volume>62</volume><issue>1</issue><spage>26</spage><epage>49</epage><pages>26-49</pages><issn>0026-265X</issn><eissn>1095-9149</eissn><coden>MICJAN</coden><abstract>Three different amounts (i.e., selector coverages) of ristocetin A macrocyclic antibiotic have been covalently bonded to silica gel, packed in stainless steel columns, and evaluated as chiral stationary phases (CSPs) in high-performance liquid chromatography (HPLC). The three columns (i.e., CSPs) were examined and compared in the reversed-phase, polar-organic, and normal-phase modes. The retention behavior (k′), selectivity (α), resolution (RS), and number of theoretical plates (N) for all three columns are discussed and compared in each mobile phase mode. The coverage of the chiral selector had a profound effect on the chromatographic behavior of the more retained enantiomers. A special mobile phase composition (i.e., methanol plus very small amounts of acid and base modifiers) resulted in improved baseline separations, without greatly affecting the analysis times, for all chiral analytes evaluated. The column stabilities were excellent when switching between different mobile phase modes.</abstract><cop>New York, NY</cop><pub>Elsevier B.V</pub><doi>10.1006/mchj.1999.1730</doi><tpages>24</tpages></addata></record> |
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| ispartof | Microchemical journal, 1999-05, Vol.62 (1), p.26-49 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | amino acids Aminoacids, peptides. Hormones. Neuropeptides Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography enantiomeric separations Exact sciences and technology Fundamental and applied biological sciences. Psychology macrocyclic antibiotic Other chromatographic methods Proteins ristocetin A selector coverage |
| title | Effect of Selector Coverage and Mobile Phase Composition on Enantiomeric Separations with Ristocetin A Chiral Stationary Phases |
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