The Expression of SR Calcium Transport ATPase and the Na+/Ca2+Exchanger are Antithetically Regulated During Mouse Cardiac Development and in Hypo/hyperthyroidism

The mouse has been used extensively for generating transgenic animal models to study cardiovascular disease. Recently, a number of transgenic mouse models have been created to investigate the importance of sarcoplasmic reticulum (SR) Ca2+transport proteins in cardiac pathophysiology. However, the ex...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of molecular and cellular cardiology 2000-03, Vol.32 (3), p.453-464
Hauptverfasser: Reed, Thomas D, Babu, Gopal J, Ji, Yong, Zilberman, Alla, Ver Heyen, Mark, Wuytack, Frank, Periasamy, Muthu
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 464
container_issue 3
container_start_page 453
container_title Journal of molecular and cellular cardiology
container_volume 32
creator Reed, Thomas D
Babu, Gopal J
Ji, Yong
Zilberman, Alla
Ver Heyen, Mark
Wuytack, Frank
Periasamy, Muthu
description The mouse has been used extensively for generating transgenic animal models to study cardiovascular disease. Recently, a number of transgenic mouse models have been created to investigate the importance of sarcoplasmic reticulum (SR) Ca2+transport proteins in cardiac pathophysiology. However, the expression and regulation of cardiac SR Ca2+ATPase and other Ca2+transport proteins have not been studied in detail in the mouse. In this study, we used multiplex RNase mapping analysis to determine SERCA2, phospholamban (PLB), and Na+/Ca2+-exchanger (NCX-1) gene expression throughout mouse heart development and in hypo/hyperthyroid animals. Our results demonstrate that the expression of SERCA2 and PLB mRNA increase eight-fold from fetal to adult stages, indicating that SR function increases with heart development. In contrast, the expression of the Na+/Ca2+-exchanger gene is two-fold higher in fetal heart compared to adult. Our study also makes the important observation that in hypothyroidic hearts the NCX-1 mRNA and protein levels were upregulated, whereas the SERCA2 mRNA/protein levels were downregulated. In hyperthyroidic hearts, however, an opposite response was identified. These findings are important and point out that the expression of NCX-1 is regulated antithetically to that of SERCA2 during heart development and in response to alterations in thyroid hormone levels.
doi_str_mv 10.1006/jmcc.1999.1095
format Article
fullrecord <record><control><sourceid>elsevier_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1006_jmcc_1999_1095</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022282899910957</els_id><sourcerecordid>S0022282899910957</sourcerecordid><originalsourceid>FETCH-LOGICAL-c284t-8f526fc164167ca35132823d60981a148de5c9ce982a09a5c1962b6a6a6856ce3</originalsourceid><addsrcrecordid>eNp1kE1v2zAMhoWhBZZ-XHfWvXAiyR-TjoGbrQO6teiys8HSdKLClgxJKeqfs386e9114IEgiOcF-TD2SYq1FKLavAyIa2mMmUdTfmCrpWW61MUZWwmhVKa00h_ZRYwvQghT5PmK_d4fie_exkAxWu-47_jPJ15Dj_Y08H0AF0cfEt_uHyESB9fyNBM_4GZTg7rZveER3IECh0B865Kdt8ki9P3En-hw6iFRy29PwboD_-5Pc0YNobWA_JZeqffjQC79zbWO302j3xynkUI6TsHb1sbhip130Ee6_tcv2a8vu319l90_fP1Wb-8zVLpIme5KVXUoq0JWnxHyUubzu3lbCaMlyEK3VKJBMlqBMFCiNJV6rmAuXVZI-SVbv-di8DEG6pox2AHC1EjRLIKbRXCzCG4WszOg3wGar3q1FJqIlhxSawNhalpv_4f-ASElg-E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The Expression of SR Calcium Transport ATPase and the Na+/Ca2+Exchanger are Antithetically Regulated During Mouse Cardiac Development and in Hypo/hyperthyroidism</title><source>Access via ScienceDirect (Elsevier)</source><creator>Reed, Thomas D ; Babu, Gopal J ; Ji, Yong ; Zilberman, Alla ; Ver Heyen, Mark ; Wuytack, Frank ; Periasamy, Muthu</creator><creatorcontrib>Reed, Thomas D ; Babu, Gopal J ; Ji, Yong ; Zilberman, Alla ; Ver Heyen, Mark ; Wuytack, Frank ; Periasamy, Muthu</creatorcontrib><description>The mouse has been used extensively for generating transgenic animal models to study cardiovascular disease. Recently, a number of transgenic mouse models have been created to investigate the importance of sarcoplasmic reticulum (SR) Ca2+transport proteins in cardiac pathophysiology. However, the expression and regulation of cardiac SR Ca2+ATPase and other Ca2+transport proteins have not been studied in detail in the mouse. In this study, we used multiplex RNase mapping analysis to determine SERCA2, phospholamban (PLB), and Na+/Ca2+-exchanger (NCX-1) gene expression throughout mouse heart development and in hypo/hyperthyroid animals. Our results demonstrate that the expression of SERCA2 and PLB mRNA increase eight-fold from fetal to adult stages, indicating that SR function increases with heart development. In contrast, the expression of the Na+/Ca2+-exchanger gene is two-fold higher in fetal heart compared to adult. Our study also makes the important observation that in hypothyroidic hearts the NCX-1 mRNA and protein levels were upregulated, whereas the SERCA2 mRNA/protein levels were downregulated. In hyperthyroidic hearts, however, an opposite response was identified. These findings are important and point out that the expression of NCX-1 is regulated antithetically to that of SERCA2 during heart development and in response to alterations in thyroid hormone levels.</description><identifier>ISSN: 0022-2828</identifier><identifier>EISSN: 1095-8584</identifier><identifier>DOI: 10.1006/jmcc.1999.1095</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Heart development ; Na+/Ca2+-exchanger (NCX-1) ; Phospholamban ; Sarco(endo)plasmic Reticulum Ca2+ATPase (SERCA) ; Thyroid hormone</subject><ispartof>Journal of molecular and cellular cardiology, 2000-03, Vol.32 (3), p.453-464</ispartof><rights>2000 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c284t-8f526fc164167ca35132823d60981a148de5c9ce982a09a5c1962b6a6a6856ce3</citedby><cites>FETCH-LOGICAL-c284t-8f526fc164167ca35132823d60981a148de5c9ce982a09a5c1962b6a6a6856ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/jmcc.1999.1095$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Reed, Thomas D</creatorcontrib><creatorcontrib>Babu, Gopal J</creatorcontrib><creatorcontrib>Ji, Yong</creatorcontrib><creatorcontrib>Zilberman, Alla</creatorcontrib><creatorcontrib>Ver Heyen, Mark</creatorcontrib><creatorcontrib>Wuytack, Frank</creatorcontrib><creatorcontrib>Periasamy, Muthu</creatorcontrib><title>The Expression of SR Calcium Transport ATPase and the Na+/Ca2+Exchanger are Antithetically Regulated During Mouse Cardiac Development and in Hypo/hyperthyroidism</title><title>Journal of molecular and cellular cardiology</title><description>The mouse has been used extensively for generating transgenic animal models to study cardiovascular disease. Recently, a number of transgenic mouse models have been created to investigate the importance of sarcoplasmic reticulum (SR) Ca2+transport proteins in cardiac pathophysiology. However, the expression and regulation of cardiac SR Ca2+ATPase and other Ca2+transport proteins have not been studied in detail in the mouse. In this study, we used multiplex RNase mapping analysis to determine SERCA2, phospholamban (PLB), and Na+/Ca2+-exchanger (NCX-1) gene expression throughout mouse heart development and in hypo/hyperthyroid animals. Our results demonstrate that the expression of SERCA2 and PLB mRNA increase eight-fold from fetal to adult stages, indicating that SR function increases with heart development. In contrast, the expression of the Na+/Ca2+-exchanger gene is two-fold higher in fetal heart compared to adult. Our study also makes the important observation that in hypothyroidic hearts the NCX-1 mRNA and protein levels were upregulated, whereas the SERCA2 mRNA/protein levels were downregulated. In hyperthyroidic hearts, however, an opposite response was identified. These findings are important and point out that the expression of NCX-1 is regulated antithetically to that of SERCA2 during heart development and in response to alterations in thyroid hormone levels.</description><subject>Heart development</subject><subject>Na+/Ca2+-exchanger (NCX-1)</subject><subject>Phospholamban</subject><subject>Sarco(endo)plasmic Reticulum Ca2+ATPase (SERCA)</subject><subject>Thyroid hormone</subject><issn>0022-2828</issn><issn>1095-8584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kE1v2zAMhoWhBZZ-XHfWvXAiyR-TjoGbrQO6teiys8HSdKLClgxJKeqfs386e9114IEgiOcF-TD2SYq1FKLavAyIa2mMmUdTfmCrpWW61MUZWwmhVKa00h_ZRYwvQghT5PmK_d4fie_exkAxWu-47_jPJ15Dj_Y08H0AF0cfEt_uHyESB9fyNBM_4GZTg7rZveER3IECh0B865Kdt8ki9P3En-hw6iFRy29PwboD_-5Pc0YNobWA_JZeqffjQC79zbWO302j3xynkUI6TsHb1sbhip130Ee6_tcv2a8vu319l90_fP1Wb-8zVLpIme5KVXUoq0JWnxHyUubzu3lbCaMlyEK3VKJBMlqBMFCiNJV6rmAuXVZI-SVbv-di8DEG6pox2AHC1EjRLIKbRXCzCG4WszOg3wGar3q1FJqIlhxSawNhalpv_4f-ASElg-E</recordid><startdate>200003</startdate><enddate>200003</enddate><creator>Reed, Thomas D</creator><creator>Babu, Gopal J</creator><creator>Ji, Yong</creator><creator>Zilberman, Alla</creator><creator>Ver Heyen, Mark</creator><creator>Wuytack, Frank</creator><creator>Periasamy, Muthu</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200003</creationdate><title>The Expression of SR Calcium Transport ATPase and the Na+/Ca2+Exchanger are Antithetically Regulated During Mouse Cardiac Development and in Hypo/hyperthyroidism</title><author>Reed, Thomas D ; Babu, Gopal J ; Ji, Yong ; Zilberman, Alla ; Ver Heyen, Mark ; Wuytack, Frank ; Periasamy, Muthu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c284t-8f526fc164167ca35132823d60981a148de5c9ce982a09a5c1962b6a6a6856ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Heart development</topic><topic>Na+/Ca2+-exchanger (NCX-1)</topic><topic>Phospholamban</topic><topic>Sarco(endo)plasmic Reticulum Ca2+ATPase (SERCA)</topic><topic>Thyroid hormone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reed, Thomas D</creatorcontrib><creatorcontrib>Babu, Gopal J</creatorcontrib><creatorcontrib>Ji, Yong</creatorcontrib><creatorcontrib>Zilberman, Alla</creatorcontrib><creatorcontrib>Ver Heyen, Mark</creatorcontrib><creatorcontrib>Wuytack, Frank</creatorcontrib><creatorcontrib>Periasamy, Muthu</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of molecular and cellular cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reed, Thomas D</au><au>Babu, Gopal J</au><au>Ji, Yong</au><au>Zilberman, Alla</au><au>Ver Heyen, Mark</au><au>Wuytack, Frank</au><au>Periasamy, Muthu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Expression of SR Calcium Transport ATPase and the Na+/Ca2+Exchanger are Antithetically Regulated During Mouse Cardiac Development and in Hypo/hyperthyroidism</atitle><jtitle>Journal of molecular and cellular cardiology</jtitle><date>2000-03</date><risdate>2000</risdate><volume>32</volume><issue>3</issue><spage>453</spage><epage>464</epage><pages>453-464</pages><issn>0022-2828</issn><eissn>1095-8584</eissn><abstract>The mouse has been used extensively for generating transgenic animal models to study cardiovascular disease. Recently, a number of transgenic mouse models have been created to investigate the importance of sarcoplasmic reticulum (SR) Ca2+transport proteins in cardiac pathophysiology. However, the expression and regulation of cardiac SR Ca2+ATPase and other Ca2+transport proteins have not been studied in detail in the mouse. In this study, we used multiplex RNase mapping analysis to determine SERCA2, phospholamban (PLB), and Na+/Ca2+-exchanger (NCX-1) gene expression throughout mouse heart development and in hypo/hyperthyroid animals. Our results demonstrate that the expression of SERCA2 and PLB mRNA increase eight-fold from fetal to adult stages, indicating that SR function increases with heart development. In contrast, the expression of the Na+/Ca2+-exchanger gene is two-fold higher in fetal heart compared to adult. Our study also makes the important observation that in hypothyroidic hearts the NCX-1 mRNA and protein levels were upregulated, whereas the SERCA2 mRNA/protein levels were downregulated. In hyperthyroidic hearts, however, an opposite response was identified. These findings are important and point out that the expression of NCX-1 is regulated antithetically to that of SERCA2 during heart development and in response to alterations in thyroid hormone levels.</abstract><pub>Elsevier Ltd</pub><doi>10.1006/jmcc.1999.1095</doi><tpages>12</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-2828
ispartof Journal of molecular and cellular cardiology, 2000-03, Vol.32 (3), p.453-464
issn 0022-2828
1095-8584
language eng
recordid cdi_crossref_primary_10_1006_jmcc_1999_1095
source Access via ScienceDirect (Elsevier)
subjects Heart development
Na+/Ca2+-exchanger (NCX-1)
Phospholamban
Sarco(endo)plasmic Reticulum Ca2+ATPase (SERCA)
Thyroid hormone
title The Expression of SR Calcium Transport ATPase and the Na+/Ca2+Exchanger are Antithetically Regulated During Mouse Cardiac Development and in Hypo/hyperthyroidism
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T02%3A38%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-elsevier_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Expression%20of%20SR%20Calcium%20Transport%20ATPase%20and%20the%20Na+/Ca2+Exchanger%20are%20Antithetically%20Regulated%20During%20Mouse%20Cardiac%20Development%20and%20in%20Hypo/hyperthyroidism&rft.jtitle=Journal%20of%20molecular%20and%20cellular%20cardiology&rft.au=Reed,%20Thomas%20D&rft.date=2000-03&rft.volume=32&rft.issue=3&rft.spage=453&rft.epage=464&rft.pages=453-464&rft.issn=0022-2828&rft.eissn=1095-8584&rft_id=info:doi/10.1006/jmcc.1999.1095&rft_dat=%3Celsevier_cross%3ES0022282899910957%3C/elsevier_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_els_id=S0022282899910957&rfr_iscdi=true