Identification of Putative Active-site Residues in the DNase Domain of Colicin E9 by Random Mutagenesis

We have used random mutagenesis to identify putative active-site residues in the C-terminal cytotoxic endonuclease domain of the bacterial toxin colicin E9. Six single-site mutations in the DNase domain were isolated which destroyed the toxic action of the colicin. DNA sequencing identified the muta...

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