Human NicotinamideN-Methyltransferase Gene: Molecular Cloning, Structural Characterization and Chromosomal Localization

Human NicotinamideN-Methyltransferase Gene: Molecular Cloning, Structural Characterization and Chromosomal Localization

Genomic DNA clones for nicotinamideN-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it poss...

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Veröffentlicht in:Genomics (San Diego, Calif.) Calif.), 1995-10, Vol.29 (3), p.555-561
Hauptverfasser: AKSOY, SAIME, BRANDRIFF, BRIGITTE F., WARD, AVIVA, LITTLE, PETER F.R., WEINSHILBOUM, RICHARD M.
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container_issue 3
container_start_page 555
container_title Genomics (San Diego, Calif.)
container_volume 29
creator AKSOY, SAIME
BRANDRIFF, BRIGITTE F.
WARD, AVIVA
LITTLE, PETER F.R.
WEINSHILBOUM, RICHARD M.
description Genomic DNA clones for nicotinamideN-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it possible to determine the structure of the human NNMT gene. The gene was approximately 16.5 kb in length and consisted of 3 exons and 2 introns. Transcription initiation for the NNMT gene occurred 105–109 nucleotides 5′-upstream from the cDNA translation initiation codon on the basis of the results of both primer extension and 5′-rapid amplification of cDNA ends.NNMTmapped to chromosome band 11q23.1 by fluorescencein situhybridization.
doi_str_mv 10.1006/geno.1995.9966
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title Human NicotinamideN-Methyltransferase Gene: Molecular Cloning, Structural Characterization and Chromosomal Localization
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