Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3
The responses of cutaneous Ca2+transport to parathyroid hormone, calcitonin, and vitamin D3were evaluated in intactRana pipiensand isolated skins from this species. Parathyroid hormone (12 pmol g−1) stimulated Ca2+influx in intact frogs but not in isolated skin (10–100 pmol ml−1) unless the frogs ha...
Gespeichert in:
| Veröffentlicht in: | General and comparative endocrinology 1998-11, Vol.112 (2), p.191-199 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 199 |
|---|---|
| container_issue | 2 |
| container_start_page | 191 |
| container_title | General and comparative endocrinology |
| container_volume | 112 |
| creator | Stiffler, Daniel F. Yee, J.Christopher Tefft, J.Denise |
| description | The responses of cutaneous Ca2+transport to parathyroid hormone, calcitonin, and vitamin D3were evaluated in intactRana pipiensand isolated skins from this species. Parathyroid hormone (12 pmol g−1) stimulated Ca2+influx in intact frogs but not in isolated skin (10–100 pmol ml−1) unless the frogs had been pretreated with vitamin D3injections (100–300 μg day−1) for 3 days prior to isolating the skins for flux measurements. Pretreatment of intact frogs with vitamin D3for 3 days (100 μg day−1) also resulted in greater responses to PTH (6–12 pmol g−1). Vitamin D3(most likely after conversion to 1,25(OH)2D3) had a direct stimulatory effect on Ca2+transport in isolated skins after three daily doses of 500 μg of D3. Calcitonin also stimulated Ca2+influx in both intact frogs (24 pmol g−1) and isolated skins (25–100 pmol ml−1). We conclude that parathyroid hormone, facilitated by vitamin D, stimulates Ca2+influx in the skin ofR. pipiens. We also conclude that calcitonin is stimulatory for cutaneous Ca2+influx in this species. |
| doi_str_mv | 10.1006/gcen.1998.7161 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1006_gcen_1998_7161</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0016648098971611</els_id><sourcerecordid>9784302</sourcerecordid><originalsourceid>FETCH-LOGICAL-c254t-a08b7b10268bfbcdee8fa12740a995b65dc0b93c4005b4d5508af4feb61fb08f3</originalsourceid><addsrcrecordid>eNp1kE1LwzAYx4Moc06v3oR8gLU-6UuaHmU6Nxgoc3otSZrM6JqUpBP27W3Z8ObpOfxf-D8_hG4JxASA3m-lsjEpSxYXhJIzNCZQ5hFlGZyjMQChEc0YXKKrEL4AIE8pGaFRWbAshWSMmrUKrbNBBew0nnu3xW_fxk7X3HLcmtYoG6Z4xnfS7Bu8dBZvPLd9xHe4c_iVe959HrwzNV443zirTu7O2b4Gc1vjD9Pxxlj8mF6jC813Qd2c7gS9z582s0W0enlezh5WkUzyrIs4MFEIAgllQgtZK8U0J0mRAS_LXNC8liDKVGb9PyKr8xwY15lWghItgOl0guJjr_QuBK901XrTcH-oCFQDtmrAVg3YqgFbH7g7Btq9aFT9Zz9x6nV21FW_-scoXwXZo5GqNl7Jrqqd-a_6F8fsfNo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Stiffler, Daniel F. ; Yee, J.Christopher ; Tefft, J.Denise</creator><creatorcontrib>Stiffler, Daniel F. ; Yee, J.Christopher ; Tefft, J.Denise</creatorcontrib><description>The responses of cutaneous Ca2+transport to parathyroid hormone, calcitonin, and vitamin D3were evaluated in intactRana pipiensand isolated skins from this species. Parathyroid hormone (12 pmol g−1) stimulated Ca2+influx in intact frogs but not in isolated skin (10–100 pmol ml−1) unless the frogs had been pretreated with vitamin D3injections (100–300 μg day−1) for 3 days prior to isolating the skins for flux measurements. Pretreatment of intact frogs with vitamin D3for 3 days (100 μg day−1) also resulted in greater responses to PTH (6–12 pmol g−1). Vitamin D3(most likely after conversion to 1,25(OH)2D3) had a direct stimulatory effect on Ca2+transport in isolated skins after three daily doses of 500 μg of D3. Calcitonin also stimulated Ca2+influx in both intact frogs (24 pmol g−1) and isolated skins (25–100 pmol ml−1). We conclude that parathyroid hormone, facilitated by vitamin D, stimulates Ca2+influx in the skin ofR. pipiens. We also conclude that calcitonin is stimulatory for cutaneous Ca2+influx in this species.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1006/gcen.1998.7161</identifier><identifier>PMID: 9784302</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Biological Transport - drug effects ; Calcitonin - administration & dosage ; Calcitonin - pharmacology ; Calcium - metabolism ; Cholecalciferol - administration & dosage ; Cholecalciferol - pharmacology ; Dose-Response Relationship, Drug ; Drug Synergism ; In Vitro Techniques ; Parathyroid Hormone - administration & dosage ; Parathyroid Hormone - pharmacology ; permissive effect of vitamin D3 ; PTH ; Rana pipiens ; Skin - drug effects ; Skin - metabolism</subject><ispartof>General and comparative endocrinology, 1998-11, Vol.112 (2), p.191-199</ispartof><rights>1998 Academic Press</rights><rights>Copyright 1998 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c254t-a08b7b10268bfbcdee8fa12740a995b65dc0b93c4005b4d5508af4feb61fb08f3</citedby><cites>FETCH-LOGICAL-c254t-a08b7b10268bfbcdee8fa12740a995b65dc0b93c4005b4d5508af4feb61fb08f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016648098971611$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9784302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stiffler, Daniel F.</creatorcontrib><creatorcontrib>Yee, J.Christopher</creatorcontrib><creatorcontrib>Tefft, J.Denise</creatorcontrib><title>Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>The responses of cutaneous Ca2+transport to parathyroid hormone, calcitonin, and vitamin D3were evaluated in intactRana pipiensand isolated skins from this species. Parathyroid hormone (12 pmol g−1) stimulated Ca2+influx in intact frogs but not in isolated skin (10–100 pmol ml−1) unless the frogs had been pretreated with vitamin D3injections (100–300 μg day−1) for 3 days prior to isolating the skins for flux measurements. Pretreatment of intact frogs with vitamin D3for 3 days (100 μg day−1) also resulted in greater responses to PTH (6–12 pmol g−1). Vitamin D3(most likely after conversion to 1,25(OH)2D3) had a direct stimulatory effect on Ca2+transport in isolated skins after three daily doses of 500 μg of D3. Calcitonin also stimulated Ca2+influx in both intact frogs (24 pmol g−1) and isolated skins (25–100 pmol ml−1). We conclude that parathyroid hormone, facilitated by vitamin D, stimulates Ca2+influx in the skin ofR. pipiens. We also conclude that calcitonin is stimulatory for cutaneous Ca2+influx in this species.</description><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Calcitonin - administration & dosage</subject><subject>Calcitonin - pharmacology</subject><subject>Calcium - metabolism</subject><subject>Cholecalciferol - administration & dosage</subject><subject>Cholecalciferol - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Synergism</subject><subject>In Vitro Techniques</subject><subject>Parathyroid Hormone - administration & dosage</subject><subject>Parathyroid Hormone - pharmacology</subject><subject>permissive effect of vitamin D3</subject><subject>PTH</subject><subject>Rana pipiens</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LwzAYx4Moc06v3oR8gLU-6UuaHmU6Nxgoc3otSZrM6JqUpBP27W3Z8ObpOfxf-D8_hG4JxASA3m-lsjEpSxYXhJIzNCZQ5hFlGZyjMQChEc0YXKKrEL4AIE8pGaFRWbAshWSMmrUKrbNBBew0nnu3xW_fxk7X3HLcmtYoG6Z4xnfS7Bu8dBZvPLd9xHe4c_iVe959HrwzNV443zirTu7O2b4Gc1vjD9Pxxlj8mF6jC813Qd2c7gS9z582s0W0enlezh5WkUzyrIs4MFEIAgllQgtZK8U0J0mRAS_LXNC8liDKVGb9PyKr8xwY15lWghItgOl0guJjr_QuBK901XrTcH-oCFQDtmrAVg3YqgFbH7g7Btq9aFT9Zz9x6nV21FW_-scoXwXZo5GqNl7Jrqqd-a_6F8fsfNo</recordid><startdate>199811</startdate><enddate>199811</enddate><creator>Stiffler, Daniel F.</creator><creator>Yee, J.Christopher</creator><creator>Tefft, J.Denise</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199811</creationdate><title>Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3</title><author>Stiffler, Daniel F. ; Yee, J.Christopher ; Tefft, J.Denise</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c254t-a08b7b10268bfbcdee8fa12740a995b65dc0b93c4005b4d5508af4feb61fb08f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Calcitonin - administration & dosage</topic><topic>Calcitonin - pharmacology</topic><topic>Calcium - metabolism</topic><topic>Cholecalciferol - administration & dosage</topic><topic>Cholecalciferol - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Synergism</topic><topic>In Vitro Techniques</topic><topic>Parathyroid Hormone - administration & dosage</topic><topic>Parathyroid Hormone - pharmacology</topic><topic>permissive effect of vitamin D3</topic><topic>PTH</topic><topic>Rana pipiens</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stiffler, Daniel F.</creatorcontrib><creatorcontrib>Yee, J.Christopher</creatorcontrib><creatorcontrib>Tefft, J.Denise</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stiffler, Daniel F.</au><au>Yee, J.Christopher</au><au>Tefft, J.Denise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1998-11</date><risdate>1998</risdate><volume>112</volume><issue>2</issue><spage>191</spage><epage>199</epage><pages>191-199</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><abstract>The responses of cutaneous Ca2+transport to parathyroid hormone, calcitonin, and vitamin D3were evaluated in intactRana pipiensand isolated skins from this species. Parathyroid hormone (12 pmol g−1) stimulated Ca2+influx in intact frogs but not in isolated skin (10–100 pmol ml−1) unless the frogs had been pretreated with vitamin D3injections (100–300 μg day−1) for 3 days prior to isolating the skins for flux measurements. Pretreatment of intact frogs with vitamin D3for 3 days (100 μg day−1) also resulted in greater responses to PTH (6–12 pmol g−1). Vitamin D3(most likely after conversion to 1,25(OH)2D3) had a direct stimulatory effect on Ca2+transport in isolated skins after three daily doses of 500 μg of D3. Calcitonin also stimulated Ca2+influx in both intact frogs (24 pmol g−1) and isolated skins (25–100 pmol ml−1). We conclude that parathyroid hormone, facilitated by vitamin D, stimulates Ca2+influx in the skin ofR. pipiens. We also conclude that calcitonin is stimulatory for cutaneous Ca2+influx in this species.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9784302</pmid><doi>10.1006/gcen.1998.7161</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0016-6480 |
| ispartof | General and comparative endocrinology, 1998-11, Vol.112 (2), p.191-199 |
| issn | 0016-6480 1095-6840 |
| language | eng |
| recordid | cdi_crossref_primary_10_1006_gcen_1998_7161 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological Transport - drug effects Calcitonin - administration & dosage Calcitonin - pharmacology Calcium - metabolism Cholecalciferol - administration & dosage Cholecalciferol - pharmacology Dose-Response Relationship, Drug Drug Synergism In Vitro Techniques Parathyroid Hormone - administration & dosage Parathyroid Hormone - pharmacology permissive effect of vitamin D3 PTH Rana pipiens Skin - drug effects Skin - metabolism |
| title | Responses of Frog Skin,Rana pipiens, Calcium Ion Transport to Parathyroid Hormone, Calcitonin, and Vitamin D3 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T10%3A01%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Responses%20of%20Frog%20Skin,Rana%20pipiens,%20Calcium%20Ion%20Transport%20to%20Parathyroid%20Hormone,%20Calcitonin,%20and%20Vitamin%20D3&rft.jtitle=General%20and%20comparative%20endocrinology&rft.au=Stiffler,%20Daniel%20F.&rft.date=1998-11&rft.volume=112&rft.issue=2&rft.spage=191&rft.epage=199&rft.pages=191-199&rft.issn=0016-6480&rft.eissn=1095-6840&rft_id=info:doi/10.1006/gcen.1998.7161&rft_dat=%3Cpubmed_cross%3E9784302%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/9784302&rft_els_id=S0016648098971611&rfr_iscdi=true |