Further Studies on Physicochemical Properties of Mitochondrial Membranes during the Formation Process of Megamitochondria in the Rat Liver by Hydrazine
Physicochemical properties of lipids extracted from hepatic mitochondria of rats fed with 1% hydrazine diet for 3 days (3H-mitochondria) and 7 days (7H-mitochondria) were studied using differential scanning calorimetry and the fluorescence polarization measurements. Results obtained from lipids extr...
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| Veröffentlicht in: | Experimental and molecular pathology 1994-10, Vol.61 (2), p.134-151 |
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| creator | Adachi, Kayo Matsuhashi, Tatsuo Nishizawa, Yuji Usukura, Jiro Momota, Michihiko Popinigis, Jerzy Wakabayashi, Takashi |
| description | Physicochemical properties of lipids extracted from hepatic mitochondria of rats fed with 1% hydrazine diet for 3 days (3H-mitochondria) and 7 days (7H-mitochondria) were studied using differential scanning calorimetry and the fluorescence polarization measurements. Results obtained from lipids extracted from 3H-mitochondria were as follows: (1) Thermotropic phase transition temperatures (
T
m) were elevated with a distinct increase in transition enthalpy (Δ
H) compared to those of the control due to increases in relative amounts of phosphatidylethanolamine (PE) and phosphatidylserine (PS) in phospholipid species. (2)
T
m in lipids and phospholipids extracted from 3H-mitochondria was elevated with decreases in Δ
H after the addition of Ca
2+, whereas Δ
H of the control was increased after the addition of Ca
2+. (3) Phosphatidylcholine (PC) liposomes containing PE or PS at concentrations corresponding to PE/PC or PS/PC ratio in 3H-mitochondria showed elevations of transition temperatures with decreases in Δ
H values after the addition of Ca
2+, whereas those containing PE or PS ratio at concentrations corresponding to PE/PC or PS/PC in the control mitochondria showed increases in Δ
H values after the addition of Ca
2+. (4) There were essentially no changes in membrane fluidity between lipids extracted from 3H-mitochondria and those extracted from the control mitochondria. Addition of Ca
2+ to lipids extracted from 3H-mitochondria, however, caused elevation of membrane fluidity while those extracted from the control mitochondria showed no changes in membrane fluidity after the addition of Ca
2+. (5) Increases in the ratio of unsaturated fatty acids to saturated fatty acids (
U/S ratio) in phospholipids extracted from 3H-mitochondria were found to be mainly due to those in PS. These data may suggest that hydrazine modifies the membrane of mitochondria favorable for the membrane fusion via enhanced acidic phospholipid-Ca
2+ interactions. |
| doi_str_mv | 10.1006/exmp.1994.1032 |
| format | Article |
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T
m) were elevated with a distinct increase in transition enthalpy (Δ
H) compared to those of the control due to increases in relative amounts of phosphatidylethanolamine (PE) and phosphatidylserine (PS) in phospholipid species. (2)
T
m in lipids and phospholipids extracted from 3H-mitochondria was elevated with decreases in Δ
H after the addition of Ca
2+, whereas Δ
H of the control was increased after the addition of Ca
2+. (3) Phosphatidylcholine (PC) liposomes containing PE or PS at concentrations corresponding to PE/PC or PS/PC ratio in 3H-mitochondria showed elevations of transition temperatures with decreases in Δ
H values after the addition of Ca
2+, whereas those containing PE or PS ratio at concentrations corresponding to PE/PC or PS/PC in the control mitochondria showed increases in Δ
H values after the addition of Ca
2+. (4) There were essentially no changes in membrane fluidity between lipids extracted from 3H-mitochondria and those extracted from the control mitochondria. Addition of Ca
2+ to lipids extracted from 3H-mitochondria, however, caused elevation of membrane fluidity while those extracted from the control mitochondria showed no changes in membrane fluidity after the addition of Ca
2+. (5) Increases in the ratio of unsaturated fatty acids to saturated fatty acids (
U/S ratio) in phospholipids extracted from 3H-mitochondria were found to be mainly due to those in PS. These data may suggest that hydrazine modifies the membrane of mitochondria favorable for the membrane fusion via enhanced acidic phospholipid-Ca
2+ interactions.</description><identifier>ISSN: 0014-4800</identifier><identifier>EISSN: 1096-0945</identifier><identifier>DOI: 10.1006/exmp.1994.1032</identifier><identifier>PMID: 7859829</identifier><identifier>CODEN: EXMPA6</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Calorimetry, Differential Scanning ; Chemical Phenomena ; Chemistry, Physical ; Digestive system ; Fatty Acids - analysis ; Fluorescence Polarization ; Hydrazines - pharmacology ; Intracellular Membranes - chemistry ; Intracellular Membranes - drug effects ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Membrane Fluidity ; Mitochondria, Liver - chemistry ; Mitochondria, Liver - drug effects ; Mitochondria, Liver - pathology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Phospholipids - analysis ; Rats ; Rats, Wistar ; Submitochondrial Particles - chemistry ; Submitochondrial Particles - drug effects</subject><ispartof>Experimental and molecular pathology, 1994-10, Vol.61 (2), p.134-151</ispartof><rights>1994 Academic Press</rights><rights>1995 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-9bfde38610eade534ec01bb36f4fb382e8d5f2c81968bdf05c5910f1a4d839e43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S001448008471032X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3463682$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7859829$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adachi, Kayo</creatorcontrib><creatorcontrib>Matsuhashi, Tatsuo</creatorcontrib><creatorcontrib>Nishizawa, Yuji</creatorcontrib><creatorcontrib>Usukura, Jiro</creatorcontrib><creatorcontrib>Momota, Michihiko</creatorcontrib><creatorcontrib>Popinigis, Jerzy</creatorcontrib><creatorcontrib>Wakabayashi, Takashi</creatorcontrib><title>Further Studies on Physicochemical Properties of Mitochondrial Membranes during the Formation Process of Megamitochondria in the Rat Liver by Hydrazine</title><title>Experimental and molecular pathology</title><addtitle>Exp Mol Pathol</addtitle><description>Physicochemical properties of lipids extracted from hepatic mitochondria of rats fed with 1% hydrazine diet for 3 days (3H-mitochondria) and 7 days (7H-mitochondria) were studied using differential scanning calorimetry and the fluorescence polarization measurements. Results obtained from lipids extracted from 3H-mitochondria were as follows: (1) Thermotropic phase transition temperatures (
T
m) were elevated with a distinct increase in transition enthalpy (Δ
H) compared to those of the control due to increases in relative amounts of phosphatidylethanolamine (PE) and phosphatidylserine (PS) in phospholipid species. (2)
T
m in lipids and phospholipids extracted from 3H-mitochondria was elevated with decreases in Δ
H after the addition of Ca
2+, whereas Δ
H of the control was increased after the addition of Ca
2+. (3) Phosphatidylcholine (PC) liposomes containing PE or PS at concentrations corresponding to PE/PC or PS/PC ratio in 3H-mitochondria showed elevations of transition temperatures with decreases in Δ
H values after the addition of Ca
2+, whereas those containing PE or PS ratio at concentrations corresponding to PE/PC or PS/PC in the control mitochondria showed increases in Δ
H values after the addition of Ca
2+. (4) There were essentially no changes in membrane fluidity between lipids extracted from 3H-mitochondria and those extracted from the control mitochondria. Addition of Ca
2+ to lipids extracted from 3H-mitochondria, however, caused elevation of membrane fluidity while those extracted from the control mitochondria showed no changes in membrane fluidity after the addition of Ca
2+. (5) Increases in the ratio of unsaturated fatty acids to saturated fatty acids (
U/S ratio) in phospholipids extracted from 3H-mitochondria were found to be mainly due to those in PS. These data may suggest that hydrazine modifies the membrane of mitochondria favorable for the membrane fusion via enhanced acidic phospholipid-Ca
2+ interactions.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calorimetry, Differential Scanning</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Digestive system</subject><subject>Fatty Acids - analysis</subject><subject>Fluorescence Polarization</subject><subject>Hydrazines - pharmacology</subject><subject>Intracellular Membranes - chemistry</subject><subject>Intracellular Membranes - drug effects</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Fluidity</subject><subject>Mitochondria, Liver - chemistry</subject><subject>Mitochondria, Liver - drug effects</subject><subject>Mitochondria, Liver - pathology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Phospholipids - analysis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Submitochondrial Particles - chemistry</subject><subject>Submitochondrial Particles - drug effects</subject><issn>0014-4800</issn><issn>1096-0945</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKxDAUhoMoOl627oQs3HY8adKaLEUcFUYUL-uSJidOxLZD0hkcX8TXNXUGceMqhP_Cfz5CjhmMGUB5hh_NfMyUEunL8y0yYqDKDJQotskIgIlMSIA9sh_jGwAoYPku2T2XhZK5GpGvySL0Mwz0qV9Yj5F2LX2YraI3nZlh441-pw-hm2Pof1RH73yfpK61wSftDps66DZJdhF8-0pTGZ10odG9H6pCZzCuc_iqmz9Z6tsf86Pu6dQv04R6RW9WNuhP3-Ih2XH6PeLR5j0gL5Or58ubbHp_fXt5Mc0ML2WfqdpZ5LJkgNpiwQUaYHXNSydczWWO0hYuN5KpUtbWQWEKxcAxLazkCgU_ION1rwldjAFdNQ--0WFVMagGwNUAuBoAVwPgFDhZB-aLukH7a98QTfrpRtcxwXMJjvHx18ZFmYYPNXJtw3Tc0mOoovHYGrQ-oOkr2_n_FnwDDkebDQ</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>Adachi, Kayo</creator><creator>Matsuhashi, Tatsuo</creator><creator>Nishizawa, Yuji</creator><creator>Usukura, Jiro</creator><creator>Momota, Michihiko</creator><creator>Popinigis, Jerzy</creator><creator>Wakabayashi, Takashi</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19941001</creationdate><title>Further Studies on Physicochemical Properties of Mitochondrial Membranes during the Formation Process of Megamitochondria in the Rat Liver by Hydrazine</title><author>Adachi, Kayo ; Matsuhashi, Tatsuo ; Nishizawa, Yuji ; Usukura, Jiro ; Momota, Michihiko ; Popinigis, Jerzy ; Wakabayashi, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-9bfde38610eade534ec01bb36f4fb382e8d5f2c81968bdf05c5910f1a4d839e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calorimetry, Differential Scanning</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Digestive system</topic><topic>Fatty Acids - analysis</topic><topic>Fluorescence Polarization</topic><topic>Hydrazines - pharmacology</topic><topic>Intracellular Membranes - chemistry</topic><topic>Intracellular Membranes - drug effects</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Fluidity</topic><topic>Mitochondria, Liver - chemistry</topic><topic>Mitochondria, Liver - drug effects</topic><topic>Mitochondria, Liver - pathology</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Phospholipids - analysis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Submitochondrial Particles - chemistry</topic><topic>Submitochondrial Particles - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adachi, Kayo</creatorcontrib><creatorcontrib>Matsuhashi, Tatsuo</creatorcontrib><creatorcontrib>Nishizawa, Yuji</creatorcontrib><creatorcontrib>Usukura, Jiro</creatorcontrib><creatorcontrib>Momota, Michihiko</creatorcontrib><creatorcontrib>Popinigis, Jerzy</creatorcontrib><creatorcontrib>Wakabayashi, Takashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Experimental and molecular pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adachi, Kayo</au><au>Matsuhashi, Tatsuo</au><au>Nishizawa, Yuji</au><au>Usukura, Jiro</au><au>Momota, Michihiko</au><au>Popinigis, Jerzy</au><au>Wakabayashi, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Further Studies on Physicochemical Properties of Mitochondrial Membranes during the Formation Process of Megamitochondria in the Rat Liver by Hydrazine</atitle><jtitle>Experimental and molecular pathology</jtitle><addtitle>Exp Mol Pathol</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>61</volume><issue>2</issue><spage>134</spage><epage>151</epage><pages>134-151</pages><issn>0014-4800</issn><eissn>1096-0945</eissn><coden>EXMPA6</coden><abstract>Physicochemical properties of lipids extracted from hepatic mitochondria of rats fed with 1% hydrazine diet for 3 days (3H-mitochondria) and 7 days (7H-mitochondria) were studied using differential scanning calorimetry and the fluorescence polarization measurements. Results obtained from lipids extracted from 3H-mitochondria were as follows: (1) Thermotropic phase transition temperatures (
T
m) were elevated with a distinct increase in transition enthalpy (Δ
H) compared to those of the control due to increases in relative amounts of phosphatidylethanolamine (PE) and phosphatidylserine (PS) in phospholipid species. (2)
T
m in lipids and phospholipids extracted from 3H-mitochondria was elevated with decreases in Δ
H after the addition of Ca
2+, whereas Δ
H of the control was increased after the addition of Ca
2+. (3) Phosphatidylcholine (PC) liposomes containing PE or PS at concentrations corresponding to PE/PC or PS/PC ratio in 3H-mitochondria showed elevations of transition temperatures with decreases in Δ
H values after the addition of Ca
2+, whereas those containing PE or PS ratio at concentrations corresponding to PE/PC or PS/PC in the control mitochondria showed increases in Δ
H values after the addition of Ca
2+. (4) There were essentially no changes in membrane fluidity between lipids extracted from 3H-mitochondria and those extracted from the control mitochondria. Addition of Ca
2+ to lipids extracted from 3H-mitochondria, however, caused elevation of membrane fluidity while those extracted from the control mitochondria showed no changes in membrane fluidity after the addition of Ca
2+. (5) Increases in the ratio of unsaturated fatty acids to saturated fatty acids (
U/S ratio) in phospholipids extracted from 3H-mitochondria were found to be mainly due to those in PS. These data may suggest that hydrazine modifies the membrane of mitochondria favorable for the membrane fusion via enhanced acidic phospholipid-Ca
2+ interactions.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>7859829</pmid><doi>10.1006/exmp.1994.1032</doi><tpages>18</tpages></addata></record> |
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| ispartof | Experimental and molecular pathology, 1994-10, Vol.61 (2), p.134-151 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Calorimetry, Differential Scanning Chemical Phenomena Chemistry, Physical Digestive system Fatty Acids - analysis Fluorescence Polarization Hydrazines - pharmacology Intracellular Membranes - chemistry Intracellular Membranes - drug effects Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Membrane Fluidity Mitochondria, Liver - chemistry Mitochondria, Liver - drug effects Mitochondria, Liver - pathology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Phospholipids - analysis Rats Rats, Wistar Submitochondrial Particles - chemistry Submitochondrial Particles - drug effects |
| title | Further Studies on Physicochemical Properties of Mitochondrial Membranes during the Formation Process of Megamitochondria in the Rat Liver by Hydrazine |
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