Activation-Induced Expression of Human Programmed Death-1 Gene in T-Lymphocytes
The Programmed Death-1 (PD-1) gene is a member of the immunoglobulin superfamily of genes. Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hP...
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| Veröffentlicht in: | Experimental cell research 1997-04, Vol.232 (1), p.25-28 |
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| container_title | Experimental cell research |
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| creator | Vibhakar, Rajeev Juan, Gloria Traganos, Frank Darzynkiewicz, Zbigniew Finger, Lawrence R. |
| description | The Programmed Death-1 (PD-1) gene is a member of the immunoglobulin superfamily of genes. Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hPD-1, seems to correlate with activation of T lymphocytes rather than apoptosis. We observed a time-dependent upregulation of hPD-1 mRNA and protein levels in Jurkat cells during phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA)-induced differentiation. Human PD-1 protein was also induced during lectin-stimulated activation of human peripheral blood mononuclear cells. Additionally, TPA stimulation of Jurkat cells induces tyrosine phosphorylation of hPD-1, putatively on its cytoplasmic tail signal transduction motif. These data suggest a role for hPD-1 during activation and differentiation of T-lymphocytes. |
| doi_str_mv | 10.1006/excr.1997.3493 |
| format | Article |
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Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hPD-1, seems to correlate with activation of T lymphocytes rather than apoptosis. We observed a time-dependent upregulation of hPD-1 mRNA and protein levels in Jurkat cells during phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA)-induced differentiation. Human PD-1 protein was also induced during lectin-stimulated activation of human peripheral blood mononuclear cells. Additionally, TPA stimulation of Jurkat cells induces tyrosine phosphorylation of hPD-1, putatively on its cytoplasmic tail signal transduction motif. 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Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hPD-1, seems to correlate with activation of T lymphocytes rather than apoptosis. We observed a time-dependent upregulation of hPD-1 mRNA and protein levels in Jurkat cells during phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA)-induced differentiation. Human PD-1 protein was also induced during lectin-stimulated activation of human peripheral blood mononuclear cells. Additionally, TPA stimulation of Jurkat cells induces tyrosine phosphorylation of hPD-1, putatively on its cytoplasmic tail signal transduction motif. These data suggest a role for hPD-1 during activation and differentiation of T-lymphocytes.</description><subject>Antigens, CD</subject><subject>Antigens, Surface - biosynthesis</subject><subject>Apoptosis Regulatory Proteins</subject><subject>Cell Differentiation</subject><subject>Humans</subject><subject>Jurkat Cells</subject><subject>Lymphocyte Activation</subject><subject>Phosphorylation</subject><subject>Programmed Cell Death 1 Receptor</subject><subject>Signal Transduction</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - metabolism</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Tyrosine - metabolism</subject><subject>Up-Regulation</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFPwzAQhS0EKqWwsiHlDzj4YseJx6qUtlKlMpTZchybGpGkstOq_fc4asXGcifdu_d09yH0DCQFQvirOWmfghBFSpmgN2gMRBCcsSy7RWNCgGFWZsU9egjhmxBSlsBHaCSAAYdijDZT3buj6l3X4lVbH7Spk_lp700IcZR0NlkeGtUmH7778qppovxmVL_DkCxMaxLXJlu8Pjf7XafPvQmP6M6qn2Cern2CPt_n29kSrzeL1Wy6xppS0WOVWV5nAiiFSluqgXAmuKGWloyZKq8YyZSthOVVrlTOChurolDbUud5wekEpZdc7bsQvLFy712j_FkCkQMYOYCRAxg5gImGl4thf6jiF3_rVxJRLy-6iVcfnfEyaGfayMN5o3tZd-6_6F8dy3JJ</recordid><startdate>19970410</startdate><enddate>19970410</enddate><creator>Vibhakar, Rajeev</creator><creator>Juan, Gloria</creator><creator>Traganos, Frank</creator><creator>Darzynkiewicz, Zbigniew</creator><creator>Finger, Lawrence R.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19970410</creationdate><title>Activation-Induced Expression of Human Programmed Death-1 Gene in T-Lymphocytes</title><author>Vibhakar, Rajeev ; Juan, Gloria ; Traganos, Frank ; Darzynkiewicz, Zbigniew ; Finger, Lawrence R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c339t-a2f6d291331bcf3c106496e3f3844eb5b402afb9f6b5aa547faa5a31df8c55763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Antigens, CD</topic><topic>Antigens, Surface - biosynthesis</topic><topic>Apoptosis Regulatory Proteins</topic><topic>Cell Differentiation</topic><topic>Humans</topic><topic>Jurkat Cells</topic><topic>Lymphocyte Activation</topic><topic>Phosphorylation</topic><topic>Programmed Cell Death 1 Receptor</topic><topic>Signal Transduction</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - metabolism</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Tyrosine - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vibhakar, Rajeev</creatorcontrib><creatorcontrib>Juan, Gloria</creatorcontrib><creatorcontrib>Traganos, Frank</creatorcontrib><creatorcontrib>Darzynkiewicz, Zbigniew</creatorcontrib><creatorcontrib>Finger, Lawrence R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vibhakar, Rajeev</au><au>Juan, Gloria</au><au>Traganos, Frank</au><au>Darzynkiewicz, Zbigniew</au><au>Finger, Lawrence R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation-Induced Expression of Human Programmed Death-1 Gene in T-Lymphocytes</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1997-04-10</date><risdate>1997</risdate><volume>232</volume><issue>1</issue><spage>25</spage><epage>28</epage><pages>25-28</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>The Programmed Death-1 (PD-1) gene is a member of the immunoglobulin superfamily of genes. Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hPD-1, seems to correlate with activation of T lymphocytes rather than apoptosis. We observed a time-dependent upregulation of hPD-1 mRNA and protein levels in Jurkat cells during phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA)-induced differentiation. Human PD-1 protein was also induced during lectin-stimulated activation of human peripheral blood mononuclear cells. Additionally, TPA stimulation of Jurkat cells induces tyrosine phosphorylation of hPD-1, putatively on its cytoplasmic tail signal transduction motif. These data suggest a role for hPD-1 during activation and differentiation of T-lymphocytes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9141617</pmid><doi>10.1006/excr.1997.3493</doi><tpages>4</tpages></addata></record> |
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| ispartof | Experimental cell research, 1997-04, Vol.232 (1), p.25-28 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Antigens, CD Antigens, Surface - biosynthesis Apoptosis Regulatory Proteins Cell Differentiation Humans Jurkat Cells Lymphocyte Activation Phosphorylation Programmed Cell Death 1 Receptor Signal Transduction T-Lymphocytes - drug effects T-Lymphocytes - metabolism Tetradecanoylphorbol Acetate - pharmacology Tyrosine - metabolism Up-Regulation |
| title | Activation-Induced Expression of Human Programmed Death-1 Gene in T-Lymphocytes |
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