Effect of Abscisic Acid, Cold Hardening, and Photoperiod on Recovery of Cryopreservedin VitroShoot Tips of Silver Birch
Recovery of cryopreservedin vitroshoot tips of silver birch (Betula pendulaRoth) was doubled by incorporating abscisic acid (ABA) in the culture medium during cold hardening of the mother shoots. The average recovery of shoot tips was over 40% after cold hardening for 28 days at +5°C under an 8/16 l...
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Veröffentlicht in: | Cryobiology 1998-02, Vol.36 (1), p.32-39 |
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description | Recovery of cryopreservedin vitroshoot tips of silver birch (Betula pendulaRoth) was doubled by incorporating abscisic acid (ABA) in the culture medium during cold hardening of the mother shoots. The average recovery of shoot tips was over 40% after cold hardening for 28 days at +5°C under an 8/16 light/dark photoperiod on medium containing 10−4M ABA. ABA was effective in combination with low temperature and short daylength only, although large genotypical differences were noted. ABA had two different effects: it enhanced cold hardening and increased callus formation during regeneration of cryopreserved shoot tips. |
doi_str_mv | 10.1006/cryo.1997.2067 |
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ABA had two different effects: it enhanced cold hardening and increased callus formation during regeneration of cryopreserved shoot tips.</description><subject>abscisic acid</subject><subject>Betula pendulaRoth</subject><subject>cold hardening</subject><subject>cryopreservation</subject><subject>long daylength</subject><subject>short daylength</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOwzAQRS0EEqWwZe0PaIIfiRMvS1QoUiUQjdhaqT2mRiWO7Kiof4-jsmU1m3vuzByE7inJKSHiQYeTz6mUVc6IqC7QjBJJMsYlu0QzQijNGCvINbqJ8YskoOLFDP2srAU9Ym_xche1i07jpXZmgRt_MHjdBQO96z8XuOsNftv70Q8QnDfY9_gdtD9COE10k7YPASKEIxjX4w83Br_dez_i1g1ximzdIaXxowt6f4uubHeIcPc356h9WrXNOtu8Pr80y02mKedVJqhktKSE8orbihVlYanUALKsRVmYkkm2q8GYguu6NoQXO1uVRhAuBBO243OUn2t18DEGsGoI7rsLJ0WJmqypyZqarKnJWgLqMwDpqKODoJIU6HX6KSRPynj3H_oLNVZzpQ</recordid><startdate>199802</startdate><enddate>199802</enddate><creator>Ryynänen, Leena</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>199802</creationdate><title>Effect of Abscisic Acid, Cold Hardening, and Photoperiod on Recovery of Cryopreservedin VitroShoot Tips of Silver Birch</title><author>Ryynänen, Leena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1337-619215101373f72454f19cee958654d5292b8edd43c88d034bf75d6036626fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>abscisic acid</topic><topic>Betula pendulaRoth</topic><topic>cold hardening</topic><topic>cryopreservation</topic><topic>long daylength</topic><topic>short daylength</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ryynänen, Leena</creatorcontrib><collection>CrossRef</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ryynänen, Leena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Abscisic Acid, Cold Hardening, and Photoperiod on Recovery of Cryopreservedin VitroShoot Tips of Silver Birch</atitle><jtitle>Cryobiology</jtitle><date>1998-02</date><risdate>1998</risdate><volume>36</volume><issue>1</issue><spage>32</spage><epage>39</epage><pages>32-39</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>Recovery of cryopreservedin vitroshoot tips of silver birch (Betula pendulaRoth) was doubled by incorporating abscisic acid (ABA) in the culture medium during cold hardening of the mother shoots. The average recovery of shoot tips was over 40% after cold hardening for 28 days at +5°C under an 8/16 light/dark photoperiod on medium containing 10−4M ABA. ABA was effective in combination with low temperature and short daylength only, although large genotypical differences were noted. ABA had two different effects: it enhanced cold hardening and increased callus formation during regeneration of cryopreserved shoot tips.</abstract><pub>Elsevier Inc</pub><doi>10.1006/cryo.1997.2067</doi><tpages>8</tpages></addata></record> |
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subjects | abscisic acid Betula pendulaRoth cold hardening cryopreservation long daylength short daylength |
title | Effect of Abscisic Acid, Cold Hardening, and Photoperiod on Recovery of Cryopreservedin VitroShoot Tips of Silver Birch |
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