EARLY PROGRESSION FROM DIMETHYL SULFOXIDE‐INDUCED G 0 /G 1 ARREST IN L 1210 CELLS

EARLY PROGRESSION FROM DIMETHYL SULFOXIDE‐INDUCED G 0 /G 1 ARREST IN L 1210 CELLS

Recently, dimethyl sulfoxide (DMSO) has been used as a convenient cryoprotectant for stem cells in stem cell transplantation using allogenic peripheral blood or umbilical cord blood. As the stem cells have a multipotency, clarification of the extent of cell proliferation after transplantation is dif...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cell biology international 2002-02, Vol.26 (2), p.211-215
Hauptverfasser: Kudo, Hideki, Nakayama, Tohru, Mano, Yoshihiro, Suzuki, Satoe, Sassa, Shuji, Sakamoto, Shinobu
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 215
container_issue 2
container_start_page 211
container_title Cell biology international
container_volume 26
creator Kudo, Hideki
Nakayama, Tohru
Mano, Yoshihiro
Suzuki, Satoe
Sassa, Shuji
Sakamoto, Shinobu
description Recently, dimethyl sulfoxide (DMSO) has been used as a convenient cryoprotectant for stem cells in stem cell transplantation using allogenic peripheral blood or umbilical cord blood. As the stem cells have a multipotency, clarification of the extent of cell proliferation after transplantation is difficult. In the present study, DMSO gradually induced G 0 /G 1 arrest in mouse leukemia L 1210 cells with good cell viability. After removal of DMSO, the cells proliferated appropriately, resulting in expression of the DNA‐synthesizing enzymes thymidylate synthase and thymidine kinase within 6h, and the cells entering into S phase within 12h. The sequence was followed by the marked activation of both enzymes within 24h and the increase of bromodeoxyuridine (BrdU) immunoreactive (S phase) cells with rapid cell proliferation within 36h. In conclusion, mouse leukemia L 1210 cells, which were treated with 1.5% DMSO for 96h, tolerated the treatment and reversed the cell cycle arrest within 36h.
doi_str_mv 10.1006/cbir.2001.0802
format Article
fullrecord <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1006_cbir_2001_0802</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1006_cbir_2001_0802</sourcerecordid><originalsourceid>FETCH-LOGICAL-c842-20a07e846842c511d4ee5e558807bd36dc292795d66286f197d68e0ca3aaa1ff3</originalsourceid><addsrcrecordid>eNotkMFOg0AYhDdGE2v16vl_Aej_L-yyHAlsKcm2GKCJPZEtLEmNRgMnbz6Cz-iTCNHTzCQzc_gYeyT0CVFuuvNl9Dki-aiQX7EVYSw8FQhxvXgpPBnH4pbdTdPL3KJQyRWrdVKZEzxVZV7pui7KA2yrcg9ZsdfN7mSgPppt-Vxk-ufruzhkx1RnkAPCJgeCpJpHDRQHMECcEFJtTH3Pbgb7OrmHf12zZqubdOeZMi_SxHidCrnH0WLkVCjn0AmiPnROOCGUwujcB7LveMyjWPRSciUHiqNeKoedDay1NAzBmvl_t934Pk2jG9qP8fJmx8-WsF2ItAuRdiHSLkSCX-XOTKQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>EARLY PROGRESSION FROM DIMETHYL SULFOXIDE‐INDUCED G 0 /G 1 ARREST IN L 1210 CELLS</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Kudo, Hideki ; Nakayama, Tohru ; Mano, Yoshihiro ; Suzuki, Satoe ; Sassa, Shuji ; Sakamoto, Shinobu</creator><creatorcontrib>Kudo, Hideki ; Nakayama, Tohru ; Mano, Yoshihiro ; Suzuki, Satoe ; Sassa, Shuji ; Sakamoto, Shinobu</creatorcontrib><description>Recently, dimethyl sulfoxide (DMSO) has been used as a convenient cryoprotectant for stem cells in stem cell transplantation using allogenic peripheral blood or umbilical cord blood. As the stem cells have a multipotency, clarification of the extent of cell proliferation after transplantation is difficult. In the present study, DMSO gradually induced G 0 /G 1 arrest in mouse leukemia L 1210 cells with good cell viability. After removal of DMSO, the cells proliferated appropriately, resulting in expression of the DNA‐synthesizing enzymes thymidylate synthase and thymidine kinase within 6h, and the cells entering into S phase within 12h. The sequence was followed by the marked activation of both enzymes within 24h and the increase of bromodeoxyuridine (BrdU) immunoreactive (S phase) cells with rapid cell proliferation within 36h. In conclusion, mouse leukemia L 1210 cells, which were treated with 1.5% DMSO for 96h, tolerated the treatment and reversed the cell cycle arrest within 36h.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1006/cbir.2001.0802</identifier><language>eng</language><ispartof>Cell biology international, 2002-02, Vol.26 (2), p.211-215</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c842-20a07e846842c511d4ee5e558807bd36dc292795d66286f197d68e0ca3aaa1ff3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids></links><search><creatorcontrib>Kudo, Hideki</creatorcontrib><creatorcontrib>Nakayama, Tohru</creatorcontrib><creatorcontrib>Mano, Yoshihiro</creatorcontrib><creatorcontrib>Suzuki, Satoe</creatorcontrib><creatorcontrib>Sassa, Shuji</creatorcontrib><creatorcontrib>Sakamoto, Shinobu</creatorcontrib><title>EARLY PROGRESSION FROM DIMETHYL SULFOXIDE‐INDUCED G 0 /G 1 ARREST IN L 1210 CELLS</title><title>Cell biology international</title><description>Recently, dimethyl sulfoxide (DMSO) has been used as a convenient cryoprotectant for stem cells in stem cell transplantation using allogenic peripheral blood or umbilical cord blood. As the stem cells have a multipotency, clarification of the extent of cell proliferation after transplantation is difficult. In the present study, DMSO gradually induced G 0 /G 1 arrest in mouse leukemia L 1210 cells with good cell viability. After removal of DMSO, the cells proliferated appropriately, resulting in expression of the DNA‐synthesizing enzymes thymidylate synthase and thymidine kinase within 6h, and the cells entering into S phase within 12h. The sequence was followed by the marked activation of both enzymes within 24h and the increase of bromodeoxyuridine (BrdU) immunoreactive (S phase) cells with rapid cell proliferation within 36h. In conclusion, mouse leukemia L 1210 cells, which were treated with 1.5% DMSO for 96h, tolerated the treatment and reversed the cell cycle arrest within 36h.</description><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNotkMFOg0AYhDdGE2v16vl_Aej_L-yyHAlsKcm2GKCJPZEtLEmNRgMnbz6Cz-iTCNHTzCQzc_gYeyT0CVFuuvNl9Dki-aiQX7EVYSw8FQhxvXgpPBnH4pbdTdPL3KJQyRWrdVKZEzxVZV7pui7KA2yrcg9ZsdfN7mSgPppt-Vxk-ufruzhkx1RnkAPCJgeCpJpHDRQHMECcEFJtTH3Pbgb7OrmHf12zZqubdOeZMi_SxHidCrnH0WLkVCjn0AmiPnROOCGUwujcB7LveMyjWPRSciUHiqNeKoedDay1NAzBmvl_t934Pk2jG9qP8fJmx8-WsF2ItAuRdiHSLkSCX-XOTKQ</recordid><startdate>200202</startdate><enddate>200202</enddate><creator>Kudo, Hideki</creator><creator>Nakayama, Tohru</creator><creator>Mano, Yoshihiro</creator><creator>Suzuki, Satoe</creator><creator>Sassa, Shuji</creator><creator>Sakamoto, Shinobu</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200202</creationdate><title>EARLY PROGRESSION FROM DIMETHYL SULFOXIDE‐INDUCED G 0 /G 1 ARREST IN L 1210 CELLS</title><author>Kudo, Hideki ; Nakayama, Tohru ; Mano, Yoshihiro ; Suzuki, Satoe ; Sassa, Shuji ; Sakamoto, Shinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c842-20a07e846842c511d4ee5e558807bd36dc292795d66286f197d68e0ca3aaa1ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kudo, Hideki</creatorcontrib><creatorcontrib>Nakayama, Tohru</creatorcontrib><creatorcontrib>Mano, Yoshihiro</creatorcontrib><creatorcontrib>Suzuki, Satoe</creatorcontrib><creatorcontrib>Sassa, Shuji</creatorcontrib><creatorcontrib>Sakamoto, Shinobu</creatorcontrib><collection>CrossRef</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kudo, Hideki</au><au>Nakayama, Tohru</au><au>Mano, Yoshihiro</au><au>Suzuki, Satoe</au><au>Sassa, Shuji</au><au>Sakamoto, Shinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EARLY PROGRESSION FROM DIMETHYL SULFOXIDE‐INDUCED G 0 /G 1 ARREST IN L 1210 CELLS</atitle><jtitle>Cell biology international</jtitle><date>2002-02</date><risdate>2002</risdate><volume>26</volume><issue>2</issue><spage>211</spage><epage>215</epage><pages>211-215</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Recently, dimethyl sulfoxide (DMSO) has been used as a convenient cryoprotectant for stem cells in stem cell transplantation using allogenic peripheral blood or umbilical cord blood. As the stem cells have a multipotency, clarification of the extent of cell proliferation after transplantation is difficult. In the present study, DMSO gradually induced G 0 /G 1 arrest in mouse leukemia L 1210 cells with good cell viability. After removal of DMSO, the cells proliferated appropriately, resulting in expression of the DNA‐synthesizing enzymes thymidylate synthase and thymidine kinase within 6h, and the cells entering into S phase within 12h. The sequence was followed by the marked activation of both enzymes within 24h and the increase of bromodeoxyuridine (BrdU) immunoreactive (S phase) cells with rapid cell proliferation within 36h. In conclusion, mouse leukemia L 1210 cells, which were treated with 1.5% DMSO for 96h, tolerated the treatment and reversed the cell cycle arrest within 36h.</abstract><doi>10.1006/cbir.2001.0802</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1065-6995
ispartof Cell biology international, 2002-02, Vol.26 (2), p.211-215
issn 1065-6995
1095-8355
language eng
recordid cdi_crossref_primary_10_1006_cbir_2001_0802
source Wiley Online Library Journals Frontfile Complete
title EARLY PROGRESSION FROM DIMETHYL SULFOXIDE‐INDUCED G 0 /G 1 ARREST IN L 1210 CELLS
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T03%3A36%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=EARLY%20PROGRESSION%20FROM%20DIMETHYL%20SULFOXIDE%E2%80%90INDUCED%20G%200%20/G%201%20ARREST%20IN%20L%201210%20CELLS&rft.jtitle=Cell%20biology%20international&rft.au=Kudo,%20Hideki&rft.date=2002-02&rft.volume=26&rft.issue=2&rft.spage=211&rft.epage=215&rft.pages=211-215&rft.issn=1065-6995&rft.eissn=1095-8355&rft_id=info:doi/10.1006/cbir.2001.0802&rft_dat=%3Ccrossref%3E10_1006_cbir_2001_0802%3C/crossref%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true