First-Generation Monoclonal Antibodies Identifying the Human Leukotriene B4 Receptor-1
The leukotriene B4 receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of thr...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-12, Vol.279 (2), p.520-525 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Pettersson, Annika Boketoft, Åke Sabirsh, Alan Nilsson, Niclas E. Kotarsky, Knut Olde, Björn Owman, Christer |
| description | The leukotriene B4 receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB4 receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB4. The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies. |
| doi_str_mv | 10.1006/bbrc.2000.3989 |
| format | Article |
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We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB4 receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB4. The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2000.3989</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>BLTR ; endogenous receptor ; HL-60 cells ; leukocytes ; leukotriene B4 receptor ; monoclonal antibodies ; recombinant receptor</subject><ispartof>Biochemical and biophysical research communications, 2000-12, Vol.279 (2), p.520-525</ispartof><rights>2000 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c267t-1a70712a50e06b2cfa816980559b7f4e8d0b5984152a9d3b223c93cdc8193fd3</citedby><cites>FETCH-LOGICAL-c267t-1a70712a50e06b2cfa816980559b7f4e8d0b5984152a9d3b223c93cdc8193fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X00939895$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Pettersson, Annika</creatorcontrib><creatorcontrib>Boketoft, Åke</creatorcontrib><creatorcontrib>Sabirsh, Alan</creatorcontrib><creatorcontrib>Nilsson, Niclas E.</creatorcontrib><creatorcontrib>Kotarsky, Knut</creatorcontrib><creatorcontrib>Olde, Björn</creatorcontrib><creatorcontrib>Owman, Christer</creatorcontrib><title>First-Generation Monoclonal Antibodies Identifying the Human Leukotriene B4 Receptor-1</title><title>Biochemical and biophysical research communications</title><description>The leukotriene B4 receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB4 receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB4. The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies.</description><subject>BLTR</subject><subject>endogenous receptor</subject><subject>HL-60 cells</subject><subject>leukocytes</subject><subject>leukotriene B4 receptor</subject><subject>monoclonal antibodies</subject><subject>recombinant receptor</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLAzEUhYMoWKtb1_kDGW8yzyxrsQ-oCFLEXcgkdzTaTkqSCv33zlC3rg73wHc5fITcc8g4QPXQtsFkAgCyXDbygkw4SGCCQ3FJJkNdMSH5-zW5ifELgPOikhPytnAhJrbEHoNOzvf02ffe7Hyvd3TWJ9d66zDStcXh6E6u_6DpE-nquNc93eDx26fgBpo-FvQVDR6SD4zfkqtO7yLe_eWUbBdP2_mKbV6W6_lsw4yo6sS4rqHmQpeAULXCdLrhlWygLGVbdwU2FtpSNgUvhZY2b4XIjcyNNQ2XeWfzKcnOb03wMQbs1CG4vQ4nxUGNUtQoRY1S1ChlAJozgMOoH4dBRTOsN2hdQJOU9e4_9BcbsGil</recordid><startdate>200012</startdate><enddate>200012</enddate><creator>Pettersson, Annika</creator><creator>Boketoft, Åke</creator><creator>Sabirsh, Alan</creator><creator>Nilsson, Niclas E.</creator><creator>Kotarsky, Knut</creator><creator>Olde, Björn</creator><creator>Owman, Christer</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200012</creationdate><title>First-Generation Monoclonal Antibodies Identifying the Human Leukotriene B4 Receptor-1</title><author>Pettersson, Annika ; Boketoft, Åke ; Sabirsh, Alan ; Nilsson, Niclas E. ; Kotarsky, Knut ; Olde, Björn ; Owman, Christer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c267t-1a70712a50e06b2cfa816980559b7f4e8d0b5984152a9d3b223c93cdc8193fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>BLTR</topic><topic>endogenous receptor</topic><topic>HL-60 cells</topic><topic>leukocytes</topic><topic>leukotriene B4 receptor</topic><topic>monoclonal antibodies</topic><topic>recombinant receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pettersson, Annika</creatorcontrib><creatorcontrib>Boketoft, Åke</creatorcontrib><creatorcontrib>Sabirsh, Alan</creatorcontrib><creatorcontrib>Nilsson, Niclas E.</creatorcontrib><creatorcontrib>Kotarsky, Knut</creatorcontrib><creatorcontrib>Olde, Björn</creatorcontrib><creatorcontrib>Owman, Christer</creatorcontrib><collection>CrossRef</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pettersson, Annika</au><au>Boketoft, Åke</au><au>Sabirsh, Alan</au><au>Nilsson, Niclas E.</au><au>Kotarsky, Knut</au><au>Olde, Björn</au><au>Owman, Christer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First-Generation Monoclonal Antibodies Identifying the Human Leukotriene B4 Receptor-1</atitle><jtitle>Biochemical and biophysical research communications</jtitle><date>2000-12</date><risdate>2000</risdate><volume>279</volume><issue>2</issue><spage>520</spage><epage>525</epage><pages>520-525</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The leukotriene B4 receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB4 receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB4. The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies.</abstract><pub>Elsevier Inc</pub><doi>10.1006/bbrc.2000.3989</doi><tpages>6</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | BLTR endogenous receptor HL-60 cells leukocytes leukotriene B4 receptor monoclonal antibodies recombinant receptor |
| title | First-Generation Monoclonal Antibodies Identifying the Human Leukotriene B4 Receptor-1 |
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