Preparation of a Monoclonal Antibody to Nϵ-(Hexanonyl)lysine: Application to the Evaluation of Protective Effects of Flavonoid Supplementation against Exercise-Induced Oxidative Stress in Rat Skeletal Muscle
The monoclonal antibody to Nϵ-(hexanonyl)lysine (HEL), a novel adduct formed by the reaction of linoleic acid hydroperoxide and lysine, has been prepared and characterized. The obtained antibody specifically recognized the HEL moiety. Using the monoclonal antibody, we evaluated the protective effect...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2000-08, Vol.274 (2), p.389-393 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kato, Yoji Miyake, Yoshiaki Yamamoto, Kanefumi Shimomura, Yoshiharu Ochi, Hirotomo Mori, Yoko Osawa, Toshihiko |
| description | The monoclonal antibody to Nϵ-(hexanonyl)lysine (HEL), a novel adduct formed by the reaction of linoleic acid hydroperoxide and lysine, has been prepared and characterized. The obtained antibody specifically recognized the HEL moiety. Using the monoclonal antibody, we evaluated the protective effects of feeding eriocitrin, which is one of flavonoids in lemon fruit, on oxidative modification induced by exercise in rats. The supplementation of eriocitrin significantly suppressed the increase in HEL in the skeletal muscle by exercise. The result suggests that the determination of HEL may be a good method for evaluation of the protective effect of beneficial food factors against oxidative stress. |
| doi_str_mv | 10.1006/bbrc.2000.3150 |
| format | Article |
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The result suggests that the determination of HEL may be a good method for evaluation of the protective effect of beneficial food factors against oxidative stress.</description><subject>adduct</subject><subject>biomarker</subject><subject>exercise</subject><subject>flavonoid</subject><subject>lipid hydroperoxide</subject><subject>monoclonal antibody</subject><subject>oxidative stress</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNp1kctOIzEQRa0RSBMeW9ZewqIztrvTidlFKDwkGBABiV2r2q4Gzxg7sp0o-TC-Y_gk3GTEjpWtcp1b13UJOeJsyBmrf7VtUEPBGBuWfMR-kAFnkhWCs2qHDHK5LoTkTz_JXox_GOO8quWAvN8FXECAZLyjvqNAb7zzynoHlk5dMq3XG5o8_f3vrTi-xDU47zb2xG6icXhKp4uFNWqL5670gnS2Arv8ErwLPqFKZpUfui7fYl89t7DKc4ym82VWwFd0aYvAMxgXE52tMSgTsbhyeqlQ09u10fCpM08BY6TG0XtIdP4XLabs9mYZlcUDstuBjXj4_9wnj-ezh7PL4vr24upsel0owSepkKNOy7JjXMh2POKTqlMSK1ELjazksoVaczmCcVXWUlQllKWQGpBVYwG8xrrcJ8Otrgo-xoBdswjmFcKm4azp82j6PJo-j6bPIwOTLYDZ1cpgaKIy6PLXTMhrabQ336EfM16W0A</recordid><startdate>20000802</startdate><enddate>20000802</enddate><creator>Kato, Yoji</creator><creator>Miyake, Yoshiaki</creator><creator>Yamamoto, Kanefumi</creator><creator>Shimomura, Yoshiharu</creator><creator>Ochi, Hirotomo</creator><creator>Mori, Yoko</creator><creator>Osawa, Toshihiko</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20000802</creationdate><title>Preparation of a Monoclonal Antibody to Nϵ-(Hexanonyl)lysine: Application to the Evaluation of Protective Effects of Flavonoid Supplementation against Exercise-Induced Oxidative Stress in Rat Skeletal Muscle</title><author>Kato, Yoji ; Miyake, Yoshiaki ; Yamamoto, Kanefumi ; Shimomura, Yoshiharu ; Ochi, Hirotomo ; Mori, Yoko ; Osawa, Toshihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c218t-95fd93f0129b75184fc9e4262de0319ba6d195a74369243a3329dae0472a16e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>adduct</topic><topic>biomarker</topic><topic>exercise</topic><topic>flavonoid</topic><topic>lipid hydroperoxide</topic><topic>monoclonal antibody</topic><topic>oxidative stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kato, Yoji</creatorcontrib><creatorcontrib>Miyake, Yoshiaki</creatorcontrib><creatorcontrib>Yamamoto, Kanefumi</creatorcontrib><creatorcontrib>Shimomura, Yoshiharu</creatorcontrib><creatorcontrib>Ochi, Hirotomo</creatorcontrib><creatorcontrib>Mori, Yoko</creatorcontrib><creatorcontrib>Osawa, Toshihiko</creatorcontrib><collection>CrossRef</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kato, Yoji</au><au>Miyake, Yoshiaki</au><au>Yamamoto, Kanefumi</au><au>Shimomura, Yoshiharu</au><au>Ochi, Hirotomo</au><au>Mori, Yoko</au><au>Osawa, Toshihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of a Monoclonal Antibody to Nϵ-(Hexanonyl)lysine: Application to the Evaluation of Protective Effects of Flavonoid Supplementation against Exercise-Induced Oxidative Stress in Rat Skeletal Muscle</atitle><jtitle>Biochemical and biophysical research communications</jtitle><date>2000-08-02</date><risdate>2000</risdate><volume>274</volume><issue>2</issue><spage>389</spage><epage>393</epage><pages>389-393</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The monoclonal antibody to Nϵ-(hexanonyl)lysine (HEL), a novel adduct formed by the reaction of linoleic acid hydroperoxide and lysine, has been prepared and characterized. 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| subjects | adduct biomarker exercise flavonoid lipid hydroperoxide monoclonal antibody oxidative stress |
| title | Preparation of a Monoclonal Antibody to Nϵ-(Hexanonyl)lysine: Application to the Evaluation of Protective Effects of Flavonoid Supplementation against Exercise-Induced Oxidative Stress in Rat Skeletal Muscle |
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