Transforming Growth Factor-β1 Increases mRNA Levels of Osteoclastogenesis Inhibitory Factor in Osteoblastic/Stromal Cells and Inhibits the Survival of Murine Osteoclast-like Cells

Osteoclastogenesis inhibitory factor (OCIF), also termed osteoprotegerin (OPG), is a secreted member of the tumor necrosis factor (TNF) receptor family. It inhibits bone resorptionin vivoand osteoclast-like cell (OCL) formationin vitro.To better understand the biological role of OCIF, we first exami...

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Veröffentlicht in:Biochemical and biophysical research communications 1998-11, Vol.252 (3), p.747-752
Hauptverfasser: Murakami, Takehiko, Yamamoto, Michiko, Yamamoto, Mikio, Ono, Katsuhiro, Nishikawa, Miyuki, Nagata, Naokazu, Motoyoshi, Kazuo, Akatsu, Takuhiko
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container_issue 3
container_start_page 747
container_title Biochemical and biophysical research communications
container_volume 252
creator Murakami, Takehiko
Yamamoto, Michiko
Yamamoto, Mikio
Ono, Katsuhiro
Nishikawa, Miyuki
Nagata, Naokazu
Motoyoshi, Kazuo
Akatsu, Takuhiko
description Osteoclastogenesis inhibitory factor (OCIF), also termed osteoprotegerin (OPG), is a secreted member of the tumor necrosis factor (TNF) receptor family. It inhibits bone resorptionin vivoand osteoclast-like cell (OCL) formationin vitro.To better understand the biological role of OCIF, we first examined the effects of various osteotropic agents on OCIF mRNA levels in mouse calvarial osteoblasts. Northern blot analysis showed that stimulators of OCL formation such as 1,25-(OH)2D3, prostaglandin E2(PGE2), parathyroid hormone (PTH), and interleukin 1 (IL-1) decreased OCIF mRNA levels. In contrast, transforming growth factor (TGF)-β1 increased OCIF mRNA levels in primary osteoblasts as well as in osteoblastic/stromal cell lines. Since it was reported that both TGF-β1 and OCIF not only inhibited OCL formation but also impaired the survival of OCL by inducing apoptosisin vitro,we next examined the possible involvement of OCIF in TGF-β1-induced impairment of OCL survival. In a mouse bone marrow culture, we confirmed that addition of OCIF or TGF-β1 decreased the number of surviving OCL. Anti-OCIF IgG, which completely neutralized the effect of OCIF, partially prevented the TGF-β1-induced decrease in the number of OCL. Our results suggest that (i) downregulation of OCIF expression is one of the mechanisms for the stimulatory effects of 1,25(OH)2D3, PGE2, PTH, and IL-1 on osteoclastogenesis; and (ii) the TGF-β1-induced apoptosis of OCL is mediated, at least in part, by upregulation of OCIF expression.
doi_str_mv 10.1006/bbrc.1998.9723
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It inhibits bone resorptionin vivoand osteoclast-like cell (OCL) formationin vitro.To better understand the biological role of OCIF, we first examined the effects of various osteotropic agents on OCIF mRNA levels in mouse calvarial osteoblasts. Northern blot analysis showed that stimulators of OCL formation such as 1,25-(OH)2D3, prostaglandin E2(PGE2), parathyroid hormone (PTH), and interleukin 1 (IL-1) decreased OCIF mRNA levels. In contrast, transforming growth factor (TGF)-β1 increased OCIF mRNA levels in primary osteoblasts as well as in osteoblastic/stromal cell lines. Since it was reported that both TGF-β1 and OCIF not only inhibited OCL formation but also impaired the survival of OCL by inducing apoptosisin vitro,we next examined the possible involvement of OCIF in TGF-β1-induced impairment of OCL survival. In a mouse bone marrow culture, we confirmed that addition of OCIF or TGF-β1 decreased the number of surviving OCL. Anti-OCIF IgG, which completely neutralized the effect of OCIF, partially prevented the TGF-β1-induced decrease in the number of OCL. Our results suggest that (i) downregulation of OCIF expression is one of the mechanisms for the stimulatory effects of 1,25(OH)2D3, PGE2, PTH, and IL-1 on osteoclastogenesis; and (ii) the TGF-β1-induced apoptosis of OCL is mediated, at least in part, by upregulation of OCIF expression.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1998.9723</identifier><language>eng</language><publisher>Elsevier Inc</publisher><ispartof>Biochemical and biophysical research communications, 1998-11, Vol.252 (3), p.747-752</ispartof><rights>1998 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c267t-ce78939462e1ff59eed1c9eb489ea651e257d3921287c9ba67aea3fb2e31f3143</citedby><cites>FETCH-LOGICAL-c267t-ce78939462e1ff59eed1c9eb489ea651e257d3921287c9ba67aea3fb2e31f3143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1998.9723$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Murakami, Takehiko</creatorcontrib><creatorcontrib>Yamamoto, Michiko</creatorcontrib><creatorcontrib>Yamamoto, Mikio</creatorcontrib><creatorcontrib>Ono, Katsuhiro</creatorcontrib><creatorcontrib>Nishikawa, Miyuki</creatorcontrib><creatorcontrib>Nagata, Naokazu</creatorcontrib><creatorcontrib>Motoyoshi, Kazuo</creatorcontrib><creatorcontrib>Akatsu, Takuhiko</creatorcontrib><title>Transforming Growth Factor-β1 Increases mRNA Levels of Osteoclastogenesis Inhibitory Factor in Osteoblastic/Stromal Cells and Inhibits the Survival of Murine Osteoclast-like Cells</title><title>Biochemical and biophysical research communications</title><description>Osteoclastogenesis inhibitory factor (OCIF), also termed osteoprotegerin (OPG), is a secreted member of the tumor necrosis factor (TNF) receptor family. 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Anti-OCIF IgG, which completely neutralized the effect of OCIF, partially prevented the TGF-β1-induced decrease in the number of OCL. Our results suggest that (i) downregulation of OCIF expression is one of the mechanisms for the stimulatory effects of 1,25(OH)2D3, PGE2, PTH, and IL-1 on osteoclastogenesis; and (ii) the TGF-β1-induced apoptosis of OCL is mediated, at least in part, by upregulation of OCIF expression.</abstract><pub>Elsevier Inc</pub><doi>10.1006/bbrc.1998.9723</doi><tpages>6</tpages></addata></record>
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title Transforming Growth Factor-β1 Increases mRNA Levels of Osteoclastogenesis Inhibitory Factor in Osteoblastic/Stromal Cells and Inhibits the Survival of Murine Osteoclast-like Cells
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