Pilot Scale Expression and Purification of Soluble Protein A Tagged β1,6N-Acetylglucosaminyltransferase in CHO Cells

Expression of recombinant soluble protein A tagged mouse core 2 β6-N-acetylglucosaminyltransferase (E.C. 2.4.1.102) has been scaled-up in CHO cells using a continuously operating fluidized bed system yielding 0.3 U/day. A one step 213 fold purification by affinity chromatography on IgG-Sepharose yie...

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Veröffentlicht in:	Biochemical and biophysical research communications 1997-08, Vol.237 (3), p.653-658
Hauptverfasser:	Zeng, Steffen, Dinter, Andre, Eisenkrätzer, Detlef, Biselli, Manfred, Wandrey, Christian, Berger, Eric.G.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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