N23K, A Gene Transiently Up-Regulated during Neural Differentiation, Encodes a Precursor Protein for a Newly Identified Neuropeptide Nociceptin
We used a subtraction cloning approach to isolate a cDNA of N23K, whose mRNA and protein are transiently up-regulated in mouse NS20Y cells undergoing neurite outgrowth induced by treatment of dibutyryl cyclic AMP. N23K encodes a precursor protein for a newly discovered rat neuropeptide nociceptin. A...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1995-12, Vol.217 (2), p.539-545 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Saito, Y. Maruyama, K. Saido, T.C. Kawashima, S. |
| description | We used a subtraction cloning approach to isolate a cDNA of N23K, whose mRNA and protein are transiently up-regulated in mouse NS20Y cells undergoing neurite outgrowth induced by treatment of dibutyryl cyclic AMP. N23K encodes a precursor protein for a newly discovered rat neuropeptide nociceptin. As with other neuropeptide precursors, the N23K protein contains a putative signal peptide rich in hydrophobic amino acids at the N-terminus. N23K mRNA is present only in bl ain and spinal cord in adult mouse, and is expressed at higher levels in early postnatal brain. These findings suggest that the N23K protein functions not only as a neuropeptide precursor but also as an important component in neuronal differentiation. |
| doi_str_mv | 10.1006/bbrc.1995.2809 |
| format | Article |
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N23K encodes a precursor protein for a newly discovered rat neuropeptide nociceptin. As with other neuropeptide precursors, the N23K protein contains a putative signal peptide rich in hydrophobic amino acids at the N-terminus. N23K mRNA is present only in bl ain and spinal cord in adult mouse, and is expressed at higher levels in early postnatal brain. These findings suggest that the N23K protein functions not only as a neuropeptide precursor but also as an important component in neuronal differentiation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1995.2809</identifier><identifier>PMID: 7503733</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Western ; Cloning, Molecular ; DNA, Complementary - genetics ; Gene Expression Regulation, Developmental ; Genes ; Mice ; Molecular Sequence Data ; Neurons - cytology ; Neuropeptides - genetics ; Nociceptin ; Opioid Peptides - genetics ; Repetitive Sequences, Nucleic Acid ; RNA, Messenger - genetics ; Sequence Alignment ; Sequence Homology, Amino Acid ; Tissue Distribution ; Up-Regulation</subject><ispartof>Biochemical and biophysical research communications, 1995-12, Vol.217 (2), p.539-545</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-5a7f2565ce81edb13031956d38757a6918e0605f640dd2ea75b1dafc6818b78e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X85728094$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7503733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saito, Y.</creatorcontrib><creatorcontrib>Maruyama, K.</creatorcontrib><creatorcontrib>Saido, T.C.</creatorcontrib><creatorcontrib>Kawashima, S.</creatorcontrib><title>N23K, A Gene Transiently Up-Regulated during Neural Differentiation, Encodes a Precursor Protein for a Newly Identified Neuropeptide Nociceptin</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>We used a subtraction cloning approach to isolate a cDNA of N23K, whose mRNA and protein are transiently up-regulated in mouse NS20Y cells undergoing neurite outgrowth induced by treatment of dibutyryl cyclic AMP. N23K encodes a precursor protein for a newly discovered rat neuropeptide nociceptin. As with other neuropeptide precursors, the N23K protein contains a putative signal peptide rich in hydrophobic amino acids at the N-terminus. N23K mRNA is present only in bl ain and spinal cord in adult mouse, and is expressed at higher levels in early postnatal brain. These findings suggest that the N23K protein functions not only as a neuropeptide precursor but also as an important component in neuronal differentiation.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Neurons - cytology</subject><subject>Neuropeptides - genetics</subject><subject>Nociceptin</subject><subject>Opioid Peptides - genetics</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tissue Distribution</subject><subject>Up-Regulation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1LwzAUhoMoc05vvRPyA2w9aZd-XI4553BMkQ28K2lyMiJbW5JW2a_wL5uy4Z1XeeG870N4CLllEDKA5KEsrQxZnvMwyiA_I0MGOQQRg_E5GYJvBFHOPi7JlXOfAIyNk3xABimHOI3jIflZRfHLPZ3QOVZI11ZUzmDV7g500wTvuO12okVFVWdNtaUr7KzY0UejNVpfM6I1dXVPZ5WsFToq6JtF2VlXW5_qFk1Ftc_CL789c6H6kTae2KPqBpvWKKSrWhrZ5-qaXGixc3hzekdk8zRbT5-D5et8MZ0sAzkG3gZcpDriCZeYMVQliyFmOU9UnKU8FUnOMoQEuE7GoFSEIuUlU0LLJGNZmWYYj0h45EpbO2dRF401e2EPBYOiF1v0YotebNGL9YO746Dpyj2qv_rJpL9nxzv6X38ZtIWT3qREZbyStlC1-Q_9C_8WiMA</recordid><startdate>19951214</startdate><enddate>19951214</enddate><creator>Saito, Y.</creator><creator>Maruyama, K.</creator><creator>Saido, T.C.</creator><creator>Kawashima, S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19951214</creationdate><title>N23K, A Gene Transiently Up-Regulated during Neural Differentiation, Encodes a Precursor Protein for a Newly Identified Neuropeptide Nociceptin</title><author>Saito, Y. ; Maruyama, K. ; Saido, T.C. ; Kawashima, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-5a7f2565ce81edb13031956d38757a6918e0605f640dd2ea75b1dafc6818b78e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Neurons - cytology</topic><topic>Neuropeptides - genetics</topic><topic>Nociceptin</topic><topic>Opioid Peptides - genetics</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tissue Distribution</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saito, Y.</creatorcontrib><creatorcontrib>Maruyama, K.</creatorcontrib><creatorcontrib>Saido, T.C.</creatorcontrib><creatorcontrib>Kawashima, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saito, Y.</au><au>Maruyama, K.</au><au>Saido, T.C.</au><au>Kawashima, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>N23K, A Gene Transiently Up-Regulated during Neural Differentiation, Encodes a Precursor Protein for a Newly Identified Neuropeptide Nociceptin</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1995-12-14</date><risdate>1995</risdate><volume>217</volume><issue>2</issue><spage>539</spage><epage>545</epage><pages>539-545</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We used a subtraction cloning approach to isolate a cDNA of N23K, whose mRNA and protein are transiently up-regulated in mouse NS20Y cells undergoing neurite outgrowth induced by treatment of dibutyryl cyclic AMP. N23K encodes a precursor protein for a newly discovered rat neuropeptide nociceptin. As with other neuropeptide precursors, the N23K protein contains a putative signal peptide rich in hydrophobic amino acids at the N-terminus. N23K mRNA is present only in bl ain and spinal cord in adult mouse, and is expressed at higher levels in early postnatal brain. These findings suggest that the N23K protein functions not only as a neuropeptide precursor but also as an important component in neuronal differentiation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7503733</pmid><doi>10.1006/bbrc.1995.2809</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1995-12, Vol.217 (2), p.539-545 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_crossref_primary_10_1006_bbrc_1995_2809 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Blotting, Western Cloning, Molecular DNA, Complementary - genetics Gene Expression Regulation, Developmental Genes Mice Molecular Sequence Data Neurons - cytology Neuropeptides - genetics Nociceptin Opioid Peptides - genetics Repetitive Sequences, Nucleic Acid RNA, Messenger - genetics Sequence Alignment Sequence Homology, Amino Acid Tissue Distribution Up-Regulation |
| title | N23K, A Gene Transiently Up-Regulated during Neural Differentiation, Encodes a Precursor Protein for a Newly Identified Neuropeptide Nociceptin |
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