Expression of Cytochrome P450 mRNAs in the Colon and the Rectum in Normal Human Subjects
The expression of mRNA for three isozymes of cytochrome P450, CYP1A1, 1A2 and 3A3, was examined in biopsied samples of colon and rectum from five healthy subjects. Using RT-PCR, it was possible to detect the three CYP mRNA species at both sites in all samples examined. The expression of each CYP mRN...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1995-05, Vol.210 (2), p.350-355 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Mercurio, M.G. Shiff, S.J. Galbraith, R.A. Sassa, S. |
| description | The expression of mRNA for three isozymes of cytochrome P450, CYP1A1, 1A2 and 3A3, was examined in biopsied samples of colon and rectum from five healthy subjects. Using RT-PCR, it was possible to detect the three CYP mRNA species at both sites in all samples examined. The expression of each CYP mRNA was site-specific in that rectum had higher levels of CYP1A1 mRNA than colon, while colon had higher levels of CYP3A3 mRNA than in rectum. In contrast, there was no consistent trend in CYP1A2 mRNA expression between the two sites. Variability of alimentary CYP expression may contribute to individual differences in susceptibility to carcinogens and/or drugs. |
| doi_str_mv | 10.1006/bbrc.1995.1668 |
| format | Article |
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Using RT-PCR, it was possible to detect the three CYP mRNA species at both sites in all samples examined. The expression of each CYP mRNA was site-specific in that rectum had higher levels of CYP1A1 mRNA than colon, while colon had higher levels of CYP3A3 mRNA than in rectum. In contrast, there was no consistent trend in CYP1A2 mRNA expression between the two sites. Variability of alimentary CYP expression may contribute to individual differences in susceptibility to carcinogens and/or drugs.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1995.1668</identifier><identifier>PMID: 7755610</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aged ; Base Sequence ; Colon - enzymology ; Cytochrome P-450 CYP3A ; Cytochrome P-450 Enzyme System - biosynthesis ; Cytochrome P-450 Enzyme System - genetics ; DNA Primers ; Electrophoresis, Polyacrylamide Gel ; Female ; Humans ; Isoenzymes - biosynthesis ; Isoenzymes - genetics ; Male ; Middle Aged ; Molecular Sequence Data ; Polymerase Chain Reaction ; Rectum - enzymology ; Reference Values ; RNA, Messenger - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1995-05, Vol.210 (2), p.350-355</ispartof><rights>1995 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c254t-bd0dc8fc93152efa049ec1156685faa9fe1a3f46d522411514f5517ebc10864a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1995.1668$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7755610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mercurio, M.G.</creatorcontrib><creatorcontrib>Shiff, S.J.</creatorcontrib><creatorcontrib>Galbraith, R.A.</creatorcontrib><creatorcontrib>Sassa, S.</creatorcontrib><title>Expression of Cytochrome P450 mRNAs in the Colon and the Rectum in Normal Human Subjects</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The expression of mRNA for three isozymes of cytochrome P450, CYP1A1, 1A2 and 3A3, was examined in biopsied samples of colon and rectum from five healthy subjects. Using RT-PCR, it was possible to detect the three CYP mRNA species at both sites in all samples examined. The expression of each CYP mRNA was site-specific in that rectum had higher levels of CYP1A1 mRNA than colon, while colon had higher levels of CYP3A3 mRNA than in rectum. In contrast, there was no consistent trend in CYP1A2 mRNA expression between the two sites. Variability of alimentary CYP expression may contribute to individual differences in susceptibility to carcinogens and/or drugs.</description><subject>Aged</subject><subject>Base Sequence</subject><subject>Colon - enzymology</subject><subject>Cytochrome P-450 CYP3A</subject><subject>Cytochrome P-450 Enzyme System - biosynthesis</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>DNA Primers</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Humans</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - genetics</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Rectum - enzymology</subject><subject>Reference Values</subject><subject>RNA, Messenger - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kNFLwzAQxoMoc05ffRPyD7Tm2qRrHkeZThhTpsLeQpomrGNtRtKK--9N3fDNp-Pu--6474fQPZAYCMkey9KpGDhnMWRZfoHGQDiJEiD0Eo1JcEQJh801uvF-RwgAzfgIjaZTxjIgY7SZfx-c9r62LbYGF8fOqq2zjcZvlBHcrFczj-sWd1uNC7sPLtlWv91aq65vBm1lXSP3eNE3ssXvfbkLir9FV0buvb471wn6fJp_FIto-fr8UsyWkUoY7aKyIpXKjeIpsEQbSSjXCoCFLMxIyY0GmRqaVSxJaJgDNYzBVJcKSJ5RmU5QfLqrnPXeaSMOrm6kOwogYiAkBkJiICQGQmHh4bRw6MtGV3_2M5Kg5yddh6-_au2EV7Vula5qF4KJytb_nf4BY-Nz-g</recordid><startdate>19950516</startdate><enddate>19950516</enddate><creator>Mercurio, M.G.</creator><creator>Shiff, S.J.</creator><creator>Galbraith, R.A.</creator><creator>Sassa, S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19950516</creationdate><title>Expression of Cytochrome P450 mRNAs in the Colon and the Rectum in Normal Human Subjects</title><author>Mercurio, M.G. ; Shiff, S.J. ; Galbraith, R.A. ; Sassa, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c254t-bd0dc8fc93152efa049ec1156685faa9fe1a3f46d522411514f5517ebc10864a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Aged</topic><topic>Base Sequence</topic><topic>Colon - enzymology</topic><topic>Cytochrome P-450 CYP3A</topic><topic>Cytochrome P-450 Enzyme System - biosynthesis</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>DNA Primers</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Humans</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - genetics</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Rectum - enzymology</topic><topic>Reference Values</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mercurio, M.G.</creatorcontrib><creatorcontrib>Shiff, S.J.</creatorcontrib><creatorcontrib>Galbraith, R.A.</creatorcontrib><creatorcontrib>Sassa, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mercurio, M.G.</au><au>Shiff, S.J.</au><au>Galbraith, R.A.</au><au>Sassa, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Cytochrome P450 mRNAs in the Colon and the Rectum in Normal Human Subjects</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1995-05-16</date><risdate>1995</risdate><volume>210</volume><issue>2</issue><spage>350</spage><epage>355</epage><pages>350-355</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The expression of mRNA for three isozymes of cytochrome P450, CYP1A1, 1A2 and 3A3, was examined in biopsied samples of colon and rectum from five healthy subjects. Using RT-PCR, it was possible to detect the three CYP mRNA species at both sites in all samples examined. The expression of each CYP mRNA was site-specific in that rectum had higher levels of CYP1A1 mRNA than colon, while colon had higher levels of CYP3A3 mRNA than in rectum. In contrast, there was no consistent trend in CYP1A2 mRNA expression between the two sites. Variability of alimentary CYP expression may contribute to individual differences in susceptibility to carcinogens and/or drugs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7755610</pmid><doi>10.1006/bbrc.1995.1668</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biochemical and biophysical research communications, 1995-05, Vol.210 (2), p.350-355 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Aged Base Sequence Colon - enzymology Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System - biosynthesis Cytochrome P-450 Enzyme System - genetics DNA Primers Electrophoresis, Polyacrylamide Gel Female Humans Isoenzymes - biosynthesis Isoenzymes - genetics Male Middle Aged Molecular Sequence Data Polymerase Chain Reaction Rectum - enzymology Reference Values RNA, Messenger - metabolism |
| title | Expression of Cytochrome P450 mRNAs in the Colon and the Rectum in Normal Human Subjects |
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