Characterization of the Biological Role of Murine Erythroleukemia Cells "Differentiation-Enhancing Factor" Using Antisense Oligodeoxynucleotides
On the basis of the amino acid sequence of isolated tryptic peptides, it has been established that the differentiation enhancing factor, produced and active on murine erythroleukemia (MEL)cells, possesses a unique sequence, with no similarity to that of known proteins. Accordingly, this factor can b...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-06, Vol.193 (3), p.941-947 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Sparatore, B. Patrone, M. Passalacqua, M. Pessino, A. Falchetto, R. Melloni, E. Pontremoli, S. |
| description | On the basis of the amino acid sequence of isolated tryptic peptides, it has been established that the differentiation enhancing factor, produced and active on murine erythroleukemia (MEL)cells, possesses a unique sequence, with no similarity to that of known proteins. Accordingly, this factor can be defined as a novel biologically active peptide. An antisense oligodeoxynucleotide, deduced from the sequence of a nonadecapeptide (produced by tryptic digestion of the factor), decreases the rate and the extent of MEL cell differentiation, induced by hexamethylenebisacetamide. In these cells the amount of the factor is reduced to one third of that constitutively present in untreated cells. Exogenous addition of the factor restores cell inducibility to normal values. Taken together, these results demonstrate the presence in MEL cells of a new factor, structurally and firnctionally unrelated to any of the known biologically active peptides, and suggest its crucial role in the promotion of an initial signal, in chemically induced erythroid differentiation. |
| doi_str_mv | 10.1006/bbrc.1993.1716 |
| format | Article |
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Accordingly, this factor can be defined as a novel biologically active peptide. An antisense oligodeoxynucleotide, deduced from the sequence of a nonadecapeptide (produced by tryptic digestion of the factor), decreases the rate and the extent of MEL cell differentiation, induced by hexamethylenebisacetamide. In these cells the amount of the factor is reduced to one third of that constitutively present in untreated cells. Exogenous addition of the factor restores cell inducibility to normal values. Taken together, these results demonstrate the presence in MEL cells of a new factor, structurally and firnctionally unrelated to any of the known biologically active peptides, and suggest its crucial role in the promotion of an initial signal, in chemically induced erythroid differentiation.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1716</identifier><identifier>PMID: 8323566</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Cell Differentiation - drug effects ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Drug Resistance ; Fundamental and applied biological sciences. Psychology ; HMGB1 Protein ; Leukemia, Erythroblastic, Acute ; Leukemia, Experimental ; Mice ; Molecular and cellular biology ; Molecular Sequence Data ; Neoplasm Proteins - genetics ; Neoplasm Proteins - physiology ; Oligonucleotides, Antisense - pharmacology ; Tumor Cells, Cultured ; Vincristine - toxicity</subject><ispartof>Biochemical and biophysical research communications, 1993-06, Vol.193 (3), p.941-947</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c283t-8eac4379d8f0e6906ed33fbf798bee93fd673042199bada4ed9a0c3403482e7e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X8371716X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4826450$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8323566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sparatore, B.</creatorcontrib><creatorcontrib>Patrone, M.</creatorcontrib><creatorcontrib>Passalacqua, M.</creatorcontrib><creatorcontrib>Pessino, A.</creatorcontrib><creatorcontrib>Falchetto, R.</creatorcontrib><creatorcontrib>Melloni, E.</creatorcontrib><creatorcontrib>Pontremoli, S.</creatorcontrib><title>Characterization of the Biological Role of Murine Erythroleukemia Cells "Differentiation-Enhancing Factor" Using Antisense Oligodeoxynucleotides</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>On the basis of the amino acid sequence of isolated tryptic peptides, it has been established that the differentiation enhancing factor, produced and active on murine erythroleukemia (MEL)cells, possesses a unique sequence, with no similarity to that of known proteins. Accordingly, this factor can be defined as a novel biologically active peptide. An antisense oligodeoxynucleotide, deduced from the sequence of a nonadecapeptide (produced by tryptic digestion of the factor), decreases the rate and the extent of MEL cell differentiation, induced by hexamethylenebisacetamide. In these cells the amount of the factor is reduced to one third of that constitutively present in untreated cells. Exogenous addition of the factor restores cell inducibility to normal values. Taken together, these results demonstrate the presence in MEL cells of a new factor, structurally and firnctionally unrelated to any of the known biologically active peptides, and suggest its crucial role in the promotion of an initial signal, in chemically induced erythroid differentiation.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Drug Resistance</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HMGB1 Protein</subject><subject>Leukemia, Erythroblastic, Acute</subject><subject>Leukemia, Experimental</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasm Proteins - physiology</subject><subject>Oligonucleotides, Antisense - pharmacology</subject><subject>Tumor Cells, Cultured</subject><subject>Vincristine - toxicity</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1PAyEQhonRaP24ejMhxutWWFZ2OWqtH4mmibGJtw0LQ4tuwcDWWH-FP1nWNt48EWaeeWEehI4pGVJC-HnTBDWkQrAhLSnfQgNKBMlySoptNCCJyHJBX_bQfoyvhFBacLGLdiuWswvOB-h7NJdBqg6C_ZKd9Q57g7s54CvrWz-zSrb4ybfQlx-XwTrA47Dq5iHVlm-wsBKPoG0jPr22xkAA19nfnGzs5tIp62b4JuX7cIqnsb9dJiKCi4AnrZ15Df5z5ZaqBd9ZDfEQ7RjZRjjanAdoejN-Ht1lD5Pb-9HlQ6byinVZBVIVrBS6MgS4IBw0Y6YxpagaAMGM5iUjRZ7MNFLLArSQRLGCsKLKoQR2gIbrXBV8jAFM_R7sQoZVTUndm617s3Vvtu7NpoGT9cD7slmA_sM3KlP_bNOXMVkzod8-_mHpWV5ckIRVawzSch8WQh2VBadA2wCqq7W3__3gBxK8mE0</recordid><startdate>19930630</startdate><enddate>19930630</enddate><creator>Sparatore, B.</creator><creator>Patrone, M.</creator><creator>Passalacqua, M.</creator><creator>Pessino, A.</creator><creator>Falchetto, R.</creator><creator>Melloni, E.</creator><creator>Pontremoli, S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19930630</creationdate><title>Characterization of the Biological Role of Murine Erythroleukemia Cells "Differentiation-Enhancing Factor" Using Antisense Oligodeoxynucleotides</title><author>Sparatore, B. ; Patrone, M. ; Passalacqua, M. ; Pessino, A. ; Falchetto, R. ; Melloni, E. ; Pontremoli, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c283t-8eac4379d8f0e6906ed33fbf798bee93fd673042199bada4ed9a0c3403482e7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Drug Resistance</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HMGB1 Protein</topic><topic>Leukemia, Erythroblastic, Acute</topic><topic>Leukemia, Experimental</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasm Proteins - physiology</topic><topic>Oligonucleotides, Antisense - pharmacology</topic><topic>Tumor Cells, Cultured</topic><topic>Vincristine - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sparatore, B.</creatorcontrib><creatorcontrib>Patrone, M.</creatorcontrib><creatorcontrib>Passalacqua, M.</creatorcontrib><creatorcontrib>Pessino, A.</creatorcontrib><creatorcontrib>Falchetto, R.</creatorcontrib><creatorcontrib>Melloni, E.</creatorcontrib><creatorcontrib>Pontremoli, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sparatore, B.</au><au>Patrone, M.</au><au>Passalacqua, M.</au><au>Pessino, A.</au><au>Falchetto, R.</au><au>Melloni, E.</au><au>Pontremoli, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the Biological Role of Murine Erythroleukemia Cells "Differentiation-Enhancing Factor" Using Antisense Oligodeoxynucleotides</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-06-30</date><risdate>1993</risdate><volume>193</volume><issue>3</issue><spage>941</spage><epage>947</epage><pages>941-947</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>On the basis of the amino acid sequence of isolated tryptic peptides, it has been established that the differentiation enhancing factor, produced and active on murine erythroleukemia (MEL)cells, possesses a unique sequence, with no similarity to that of known proteins. Accordingly, this factor can be defined as a novel biologically active peptide. An antisense oligodeoxynucleotide, deduced from the sequence of a nonadecapeptide (produced by tryptic digestion of the factor), decreases the rate and the extent of MEL cell differentiation, induced by hexamethylenebisacetamide. In these cells the amount of the factor is reduced to one third of that constitutively present in untreated cells. Exogenous addition of the factor restores cell inducibility to normal values. Taken together, these results demonstrate the presence in MEL cells of a new factor, structurally and firnctionally unrelated to any of the known biologically active peptides, and suggest its crucial role in the promotion of an initial signal, in chemically induced erythroid differentiation.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8323566</pmid><doi>10.1006/bbrc.1993.1716</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1993-06, Vol.193 (3), p.941-947 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Cell Differentiation - drug effects Cell differentiation, maturation, development, hematopoiesis Cell physiology Drug Resistance Fundamental and applied biological sciences. Psychology HMGB1 Protein Leukemia, Erythroblastic, Acute Leukemia, Experimental Mice Molecular and cellular biology Molecular Sequence Data Neoplasm Proteins - genetics Neoplasm Proteins - physiology Oligonucleotides, Antisense - pharmacology Tumor Cells, Cultured Vincristine - toxicity |
| title | Characterization of the Biological Role of Murine Erythroleukemia Cells "Differentiation-Enhancing Factor" Using Antisense Oligodeoxynucleotides |
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