Translocation of Retinoblastoma Protein Associated with Tumor Cell Growth Inhibition
Immunocytochemistry revealed an association between growth inhibition and a translocation of retinoblastoma protein (pRB) to the nucleoli of U-2 osteosarcoma cells inhibited in their growth by the negative growth factor NCPI (natural cell proliferation inhibitor). Similar phenomenon was demonstrated...
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Veröffentlicht in: | Biochemical and biophysical research communications 1993-05, Vol.192 (3), p.1139-1146 |
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description | Immunocytochemistry revealed an association between growth inhibition and a translocation of retinoblastoma protein (pRB) to the nucleoli of U-2 osteosarcoma cells inhibited in their growth by the negative growth factor NCPI (natural cell proliferation inhibitor). Similar phenomenon was demonstrated by Western blot analysis and immunocytochemistry in U937 leukemic cells inhibited in their growth and induced to differentiate by 12-
O-tetradecanoylphorbol acetate (TPA). Total nuclear extract of control U937 cells gave an intense 110 KD band, while total nuclear extract of TPA treated cells produced an intense 60 KD band and a weak 110 KD band. No bands were observed for the purified nucleoli of control cells, but the purified nucleoli of TPA treated cells produced a 60 KD band. These results suggest that in the process of cell growth inhibition and differentiation, specific proteolysis of pRB and its translocation to the nucleolus may occur. |
doi_str_mv | 10.1006/bbrc.1993.1535 |
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O-tetradecanoylphorbol acetate (TPA). Total nuclear extract of control U937 cells gave an intense 110 KD band, while total nuclear extract of TPA treated cells produced an intense 60 KD band and a weak 110 KD band. No bands were observed for the purified nucleoli of control cells, but the purified nucleoli of TPA treated cells produced a 60 KD band. These results suggest that in the process of cell growth inhibition and differentiation, specific proteolysis of pRB and its translocation to the nucleolus may occur.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.1535</identifier><identifier>PMID: 8507187</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Blotting, Western ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cell Nucleolus - metabolism ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; DNA Replication - drug effects ; DNA, Neoplasm - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoenzyme Techniques ; Immunohistochemistry ; Leukemia, Monocytic, Acute ; Molecular and cellular biology ; Molecular Weight ; Osteosarcoma ; Retinoblastoma Protein - analysis ; Retinoblastoma Protein - isolation & purification ; Retinoblastoma Protein - metabolism ; Tetradecanoylphorbol Acetate - pharmacology ; Tumor Cells, Cultured</subject><ispartof>Biochemical and biophysical research communications, 1993-05, Vol.192 (3), p.1139-1146</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-6f90cc8e45866461a705590224530ed36cce1cac2a99395b794dd4a8fbbbf5133</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1993.1535$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4879364$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8507187$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rogalsky, V.</creatorcontrib><creatorcontrib>Todorov, G.</creatorcontrib><creatorcontrib>Moran, D.</creatorcontrib><title>Translocation of Retinoblastoma Protein Associated with Tumor Cell Growth Inhibition</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Immunocytochemistry revealed an association between growth inhibition and a translocation of retinoblastoma protein (pRB) to the nucleoli of U-2 osteosarcoma cells inhibited in their growth by the negative growth factor NCPI (natural cell proliferation inhibitor). Similar phenomenon was demonstrated by Western blot analysis and immunocytochemistry in U937 leukemic cells inhibited in their growth and induced to differentiate by 12-
O-tetradecanoylphorbol acetate (TPA). Total nuclear extract of control U937 cells gave an intense 110 KD band, while total nuclear extract of TPA treated cells produced an intense 60 KD band and a weak 110 KD band. No bands were observed for the purified nucleoli of control cells, but the purified nucleoli of TPA treated cells produced a 60 KD band. These results suggest that in the process of cell growth inhibition and differentiation, specific proteolysis of pRB and its translocation to the nucleolus may occur.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell Nucleolus - metabolism</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>DNA Replication - drug effects</subject><subject>DNA, Neoplasm - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Immunohistochemistry</subject><subject>Leukemia, Monocytic, Acute</subject><subject>Molecular and cellular biology</subject><subject>Molecular Weight</subject><subject>Osteosarcoma</subject><subject>Retinoblastoma Protein - analysis</subject><subject>Retinoblastoma Protein - isolation & purification</subject><subject>Retinoblastoma Protein - metabolism</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9LwzAUx4Moc06v3oQevLYmzY82xzF0DgaKVPAWkjRlka4ZSebwvzdlYzdPD977vMf7fgC4R7BAELInpbwuEOe4QBTTCzBFkMO8RJBcgilMRF5y9HUNbkL4hhAhwvgETGoKK1RXU9A0Xg6hd1pG64bMddmHiXZwqpchuq3M3r2Lxg7ZPASnrYymzQ42brJmv3U-W5i-z5beHVJnNWyssuOZW3DVyT6Yu1Odgc-X52bxmq_flqvFfJ1rzOqYs45DrWtDaM0YYUhWkFIOy5JQDE2LmdYGaalLmeJxqipO2pbIulNKdRRhPAPF8a72LgRvOrHzdiv9r0BQjHbEaEeMdsRoJy08HBd2e7U17Rk_6Ujzx9NcBi37LrnRNpwxUlccM5Kw-oiZFO7HGi-CtmbQprXe6ChaZ__74A9kpYE1</recordid><startdate>19930514</startdate><enddate>19930514</enddate><creator>Rogalsky, V.</creator><creator>Todorov, G.</creator><creator>Moran, D.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19930514</creationdate><title>Translocation of Retinoblastoma Protein Associated with Tumor Cell Growth Inhibition</title><author>Rogalsky, V. ; Todorov, G. ; Moran, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-6f90cc8e45866461a705590224530ed36cce1cac2a99395b794dd4a8fbbbf5133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell Nucleolus - metabolism</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>DNA Replication - drug effects</topic><topic>DNA, Neoplasm - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Immunohistochemistry</topic><topic>Leukemia, Monocytic, Acute</topic><topic>Molecular and cellular biology</topic><topic>Molecular Weight</topic><topic>Osteosarcoma</topic><topic>Retinoblastoma Protein - analysis</topic><topic>Retinoblastoma Protein - isolation & purification</topic><topic>Retinoblastoma Protein - metabolism</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rogalsky, V.</creatorcontrib><creatorcontrib>Todorov, G.</creatorcontrib><creatorcontrib>Moran, D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rogalsky, V.</au><au>Todorov, G.</au><au>Moran, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Translocation of Retinoblastoma Protein Associated with Tumor Cell Growth Inhibition</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-05-14</date><risdate>1993</risdate><volume>192</volume><issue>3</issue><spage>1139</spage><epage>1146</epage><pages>1139-1146</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Immunocytochemistry revealed an association between growth inhibition and a translocation of retinoblastoma protein (pRB) to the nucleoli of U-2 osteosarcoma cells inhibited in their growth by the negative growth factor NCPI (natural cell proliferation inhibitor). Similar phenomenon was demonstrated by Western blot analysis and immunocytochemistry in U937 leukemic cells inhibited in their growth and induced to differentiate by 12-
O-tetradecanoylphorbol acetate (TPA). Total nuclear extract of control U937 cells gave an intense 110 KD band, while total nuclear extract of TPA treated cells produced an intense 60 KD band and a weak 110 KD band. No bands were observed for the purified nucleoli of control cells, but the purified nucleoli of TPA treated cells produced a 60 KD band. These results suggest that in the process of cell growth inhibition and differentiation, specific proteolysis of pRB and its translocation to the nucleolus may occur.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8507187</pmid><doi>10.1006/bbrc.1993.1535</doi><tpages>8</tpages></addata></record> |
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subjects | Biological and medical sciences Blotting, Western Cell Differentiation - drug effects Cell Division - drug effects Cell Nucleolus - metabolism Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes DNA Replication - drug effects DNA, Neoplasm - biosynthesis Fundamental and applied biological sciences. Psychology Humans Immunoenzyme Techniques Immunohistochemistry Leukemia, Monocytic, Acute Molecular and cellular biology Molecular Weight Osteosarcoma Retinoblastoma Protein - analysis Retinoblastoma Protein - isolation & purification Retinoblastoma Protein - metabolism Tetradecanoylphorbol Acetate - pharmacology Tumor Cells, Cultured |
title | Translocation of Retinoblastoma Protein Associated with Tumor Cell Growth Inhibition |
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