High-Performance Liquid Chromatographic Method Combining Radiochemical and Ultraviolet Detection for Determination of Low Activities of Uridine 5′-Diphosphate-Glucuronosyltransferase

A novel reversed-phase high-performance liquid chromatographic method was developed to measure UDP-glucuronosyltransferase (UGT) activity. Radiochemical and UV detection were combined in this UDP-[14C]glucuronic acid-utilizing method which was especially aimed at determination of low activities typi...

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Veröffentlicht in:	Analytical biochemistry 2001-05, Vol.292 (2), p.178-187
Hauptverfasser:	Kaivosaari, Sanna, Salonen, Jarmo S., Mortensen, Jonna, Taskinen, Jyrki
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminobiphenyl Compounds - metabolism Amitriptyline - chemistry Amitriptyline - metabolism Chromatography, High Pressure Liquid - methods Glucuronides - metabolism Glucuronosyltransferase - analysis Glucuronosyltransferase - chemistry Glucuronosyltransferase - metabolism Humans Imidazoles - metabolism Kinetics Medetomidine - metabolism Microsomes, Liver - enzymology Nitrophenols - metabolism Radiochemistry Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization Substrate Specificity Time Factors Ultraviolet Rays
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