Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein

We describe a glucose sensor based on a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry. The GGBP fromEscherichia coliwas mutated to contain a single cysteine residue at position 26. When labeled with a sulfhydryl-reactive probe 2-(4′-iodoacetamidoanilino)naphthalene...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical biochemistry 1999-02, Vol.267 (1), p.114-120
Hauptverfasser:	Tolosa, Leah, Gryczynski, Ignacy, Eichhorn, Lisa R., Dattelbaum, Jonathan D., Castellano, Felix N., Rao, Govind, Lakowicz, Joseph R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Biosensing Techniques - economics Biosensing Techniques - methods Costs and Cost Analysis Escherichia coli - genetics Fluorescent Dyes Glucose - analysis Models, Molecular Monosaccharide Transport Proteins - chemistry Monosaccharide Transport Proteins - genetics Mutagenesis, Site-Directed Naphthalenesulfonates Protein Conformation Protein Engineering Recombinant Proteins - chemistry Recombinant Proteins - genetics Spectrometry, Fluorescence
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!