Preparation and Characterization of Three Fluorescent Labels for Proteins, Suitable for Structural Studies
Three 7-aminocoumarin derivatives, 7-carboxymethyl-amino-4-trifluoromethyl-2 H-1-benzopyran-2-one [2], 7-carboxymethylamino-4-methyl-2 H-1-benzopyran-k2-one [4], and 7-dimethylamino-4-formyl-2 H-1-benzopyran-2-one [6], were prepared. The reagents can be attached to primary amino groups of proteins....
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| Veröffentlicht in: | Analytical biochemistry 1993, Vol.209 (1), p.9-14 |
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| container_title | Analytical biochemistry |
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| creator | Forster, Y. Haas, E. |
| description | Three 7-aminocoumarin derivatives, 7-carboxymethyl-amino-4-trifluoromethyl-2
H-1-benzopyran-2-one [2], 7-carboxymethylamino-4-methyl-2
H-1-benzopyran-k2-one [4], and 7-dimethylamino-4-formyl-2
H-1-benzopyran-2-one [6], were prepared. The reagents can be attached to primary amino groups of proteins. The labeled derivatives are stable and are characterized by a strong absorption band in the near uv (300-400 nm) and an emission band in the blue region (440-510 nm) with a large Stokes′ shift. The spectral characteristics of these probes make them useful in applications of dynamic nonradiative excitation energy transfer measurements for conformational studies and analytical procedures based on simultaneous detection of labeled components in mixtures. |
| doi_str_mv | 10.1006/abio.1993.1076 |
| format | Article |
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H-1-benzopyran-2-one [2], 7-carboxymethylamino-4-methyl-2
H-1-benzopyran-k2-one [4], and 7-dimethylamino-4-formyl-2
H-1-benzopyran-2-one [6], were prepared. The reagents can be attached to primary amino groups of proteins. The labeled derivatives are stable and are characterized by a strong absorption band in the near uv (300-400 nm) and an emission band in the blue region (440-510 nm) with a large Stokes′ shift. The spectral characteristics of these probes make them useful in applications of dynamic nonradiative excitation energy transfer measurements for conformational studies and analytical procedures based on simultaneous detection of labeled components in mixtures.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1006/abio.1993.1076</identifier><identifier>PMID: 7682038</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Aprotinin - analysis ; Biological and medical sciences ; Biotechnology ; Cattle ; Chemical industry. Chemical engineering ; Coumarins - chemical synthesis ; Fluorescent Dyes - chemical synthesis ; Fundamental and applied biological sciences. Psychology ; Industrial applications and implications. Economical aspects ; Protein Conformation ; Proteins - analysis ; Spectrometry, Fluorescence ; Spectrophotometry, Ultraviolet</subject><ispartof>Analytical biochemistry, 1993, Vol.209 (1), p.9-14</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c252t-465fddff475f9612540e45d1cbdc9c8bb07a20fdffe549b6e2ed7f3ce0790e453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/abio.1993.1076$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4727600$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7682038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Forster, Y.</creatorcontrib><creatorcontrib>Haas, E.</creatorcontrib><title>Preparation and Characterization of Three Fluorescent Labels for Proteins, Suitable for Structural Studies</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Three 7-aminocoumarin derivatives, 7-carboxymethyl-amino-4-trifluoromethyl-2
H-1-benzopyran-2-one [2], 7-carboxymethylamino-4-methyl-2
H-1-benzopyran-k2-one [4], and 7-dimethylamino-4-formyl-2
H-1-benzopyran-2-one [6], were prepared. The reagents can be attached to primary amino groups of proteins. The labeled derivatives are stable and are characterized by a strong absorption band in the near uv (300-400 nm) and an emission band in the blue region (440-510 nm) with a large Stokes′ shift. The spectral characteristics of these probes make them useful in applications of dynamic nonradiative excitation energy transfer measurements for conformational studies and analytical procedures based on simultaneous detection of labeled components in mixtures.</description><subject>Animals</subject><subject>Aprotinin - analysis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cattle</subject><subject>Chemical industry. Chemical engineering</subject><subject>Coumarins - chemical synthesis</subject><subject>Fluorescent Dyes - chemical synthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Protein Conformation</subject><subject>Proteins - analysis</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LwzAUwIMoc06v3oQePNr50o-kOcpwKgwcbJ5LmrywjNqOJBX0r7e1YzdP7-v3Ho8fIbcU5hSAPcrKtnMqRNqXnJ2RKQXBYkhBnJMpAKRxwgS_JFfe7wEozXI2IRPOigTSYkr2a4cH6WSwbRPJRkeLXV-pgM7-jM3WRNudQ4yWddc69AqbEK1khbWPTOuitWsD2sY_RJvOBlnV-NfeBNep0DlZ92mnLfprcmFk7fHmGGfkY_m8XbzGq_eXt8XTKlZJnoQ4Y7nR2piM50YwmuQZYJZrqiqthCqqCrhMwPQE5pmoGCaouUkVAhcDmc7IfLyrXOu9Q1MenP2U7rukUA7OysFZOTgrB2f9wt24cOiqT9Qn_Cipn98f59IrWRsnG2X9Cct4wlkvekaKEevN4JdFV3plsVGorUMVSt3a_z74BVdBikA</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Forster, Y.</creator><creator>Haas, E.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>1993</creationdate><title>Preparation and Characterization of Three Fluorescent Labels for Proteins, Suitable for Structural Studies</title><author>Forster, Y. ; Haas, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c252t-465fddff475f9612540e45d1cbdc9c8bb07a20fdffe549b6e2ed7f3ce0790e453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Aprotinin - analysis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cattle</topic><topic>Chemical industry. Chemical engineering</topic><topic>Coumarins - chemical synthesis</topic><topic>Fluorescent Dyes - chemical synthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Protein Conformation</topic><topic>Proteins - analysis</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forster, Y.</creatorcontrib><creatorcontrib>Haas, E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Forster, Y.</au><au>Haas, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and Characterization of Three Fluorescent Labels for Proteins, Suitable for Structural Studies</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1993</date><risdate>1993</risdate><volume>209</volume><issue>1</issue><spage>9</spage><epage>14</epage><pages>9-14</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>Three 7-aminocoumarin derivatives, 7-carboxymethyl-amino-4-trifluoromethyl-2
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H-1-benzopyran-k2-one [4], and 7-dimethylamino-4-formyl-2
H-1-benzopyran-2-one [6], were prepared. The reagents can be attached to primary amino groups of proteins. The labeled derivatives are stable and are characterized by a strong absorption band in the near uv (300-400 nm) and an emission band in the blue region (440-510 nm) with a large Stokes′ shift. The spectral characteristics of these probes make them useful in applications of dynamic nonradiative excitation energy transfer measurements for conformational studies and analytical procedures based on simultaneous detection of labeled components in mixtures.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7682038</pmid><doi>10.1006/abio.1993.1076</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1993, Vol.209 (1), p.9-14 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_crossref_primary_10_1006_abio_1993_1076 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Aprotinin - analysis Biological and medical sciences Biotechnology Cattle Chemical industry. Chemical engineering Coumarins - chemical synthesis Fluorescent Dyes - chemical synthesis Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects Protein Conformation Proteins - analysis Spectrometry, Fluorescence Spectrophotometry, Ultraviolet |
| title | Preparation and Characterization of Three Fluorescent Labels for Proteins, Suitable for Structural Studies |
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