Recombinant Expression and Characterization of the Candida rugosa lip4 Lipase in Pichia pastoris: Comparison of Glycosylation, Activity, and Stability

Although Candida rugosa utilizes a nonuniversal serine codon (CUG) for leucine, it is possible to express lipase genes (LIP) in heterologous systems. After replacing the 19 CUG codons in LIP4 with serine codons by site-directed mutagenesis, a recombinant LIP4 was functionally overexpressed in Pichia...

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Veröffentlicht in:	Archives of biochemistry and biophysics 2001-03, Vol.387 (1), p.93-98
Hauptverfasser:	Tang, Shye-Jye, Shaw, Jei-Fu, Sun, Kuang-Hui, Sun, Guang-Huan, Chang, Terng-Yuan, Lin, Ching-Kai, Lo, Yuh-Chih, Lee, Guang-Chiun
Format:	Artikel
Sprache:	eng
Schlagworte:	Candida - enzymology Candida - genetics Candida rugosa Carboxylic Ester Hydrolases - genetics Carboxylic Ester Hydrolases - metabolism Codon Enzyme Stability Fungal Proteins - genetics Fungal Proteins - metabolism Glycosylation Hot Temperature Lipase Pichia - genetics Pichia pastoris Protein Processing, Post-Translational Recombinant Proteins - metabolism Serine - genetics Substrate Specificity
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