Cloning and Expression of cDNA for a Human Siaα2,3Galβ1,4GlcNA:α2,8-Sialyltransferase (hST8Sia III)

The cDNA encoding human Sia-α2,3-Gal-β1,4-GlcNAc-R:α2,8-sialyltransferase, hST8Sia III, was isolated by screening of a human brain cDNA library with polymerase chain reaction-amplified DNA probe generated from the sequence of mouse ST8Sia III (mST8Sia III) and by 5′ rapid amplification of cDNA ends...

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Veröffentlicht in:Archives of biochemistry and biophysics 1998-12, Vol.360 (1), p.41-46
Hauptverfasser: Lee, Young-Choon, Kim, Yeon-Jeong, Lee, Ki-Young, Kim, Kyoung-Sook, Kim, Bang-Ul, Kim, Hee-Nam, Kim, Cheorl-Ho, Do, Su-Il
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container_end_page 46
container_issue 1
container_start_page 41
container_title Archives of biochemistry and biophysics
container_volume 360
creator Lee, Young-Choon
Kim, Yeon-Jeong
Lee, Ki-Young
Kim, Kyoung-Sook
Kim, Bang-Ul
Kim, Hee-Nam
Kim, Cheorl-Ho
Do, Su-Il
description The cDNA encoding human Sia-α2,3-Gal-β1,4-GlcNAc-R:α2,8-sialyltransferase, hST8Sia III, was isolated by screening of a human brain cDNA library with polymerase chain reaction-amplified DNA probe generated from the sequence of mouse ST8Sia III (mST8Sia III) and by 5′ rapid amplification of cDNA ends of mRNA isolated from human brain tissues. Comparative analysis of the predicted protein-coding region between our cloned hST8Sia III and mST8Sia III showed 92 and 96% identities in the nucleotide and the amino acid sequence, respectively. The soluble hST8Sia III protein expressed in COS-7 showed an extremely high catalytic activity of transferring sialic acid through α2,8-linkage to intact fetuin glycoprotein, whereas the transferring activity was completely undetectable toward either α2,6-sialylated glycoprotein or desialylated glycoprotein acceptors. Northern analysis of hST8Sia III showed that the transcript corresponding to 11 kb was expressed in both human fetal and adult brain, while the expression of the 5.5-kb transcript was restricted to fetal liver, indicating that the expression of hST8Sia III is developmentally and tissue-specifically regulated.
doi_str_mv 10.1006/abbi.1998.0909
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subjects fetuin glycoprotein acceptor
sialic acid
Siaα2,3Galβ1,4GlcNAc:α2,8-sialyltransferase (hST8Sia III)
title Cloning and Expression of cDNA for a Human Siaα2,3Galβ1,4GlcNA:α2,8-Sialyltransferase (hST8Sia III)
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