Yeast vectors for the integration/expression of any sequence at the TYR1 locus

We have constructed new yeast vectors for targeted integration and conditional expression of any sequence at the Saccharomyces cerevisiae TYR1 locus which becomes disrupted. We show that vector integration is not neutral, causing prototrophy for tyrosine and auxotrophy for the vector's selectab...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2007-09, Vol.24 (9), p.761-766
Hauptverfasser:	Mirisola, Mario G, Colomba, Leonarda, Gallo, Alessia, Amodeo, Roberta, De Leo, Giacomo
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence DNA, Fungal - chemistry DNA, Fungal - genetics ends‐out gene targeting expression vectors Gene Expression Regulation, Fungal Genes, Reporter - genetics Genetic Vectors - chemistry Genetic Vectors - genetics Green Fluorescent Proteins - genetics heterologous expression Molecular Sequence Data Mutagenesis, Insertional - methods Polymerase Chain Reaction Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Sequence Analysis, DNA TYR1 locus Tyrosine - chemistry Tyrosine - genetics yeast integration vector
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