The relative merits of the tetO 2 and tet O 7 promoter systems for the functional analysis of heterologous genes in yeast and a compilation of essential yeast genes with tet O 2 promoter substitutions

We have generated a collection of yeast strains, each of which has an essential yeast gene under the control of the tetracycline‐responsive, tet O, promoter. Screens using first‐generation promoter‐swap strains uncovered the non‐specific responsiveness of the tet O 7 promoter to a known human transc...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2006-03, Vol.23 (4), p.325-331
Hauptverfasser:	Wishart, Jill A., Osborn, Michael, Gent, Manda E., Yen, Kuangyu, Vujovic, Zorana, Gitsham, Paul, Zhang, Nianshu, Ross Miller, J., Oliver, Stephen G.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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