An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca 2+ /Mg 2+ /Zn 2+ and Cl − with gramicidin A
The peptide, gramicidin A (GrA), has been demonstrated to interact with divalent salts (CaCl 2 , MgCl 2 , and ZnCl 2 ) using electrospray ionization mass spectrometry (ESI‐MS). The ESI‐MS analysis revealed different complexes formed due to the interaction of Val‐GrA and Ile‐GrA with divalent salts:...
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| Veröffentlicht in: | Rapid communications in mass spectrometry 2005-06, Vol.19 (11), p.1517-1521 |
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| creator | Sudhir, Putty‐Reddy Wu, Hui‐Fen Zhou, Zi‐Cong |
| description | The peptide, gramicidin A (GrA), has been demonstrated to interact with divalent salts (CaCl
2
, MgCl
2
, and ZnCl
2
) using electrospray ionization mass spectrometry (ESI‐MS). The ESI‐MS analysis revealed different complexes formed due to the interaction of Val‐GrA and Ile‐GrA with divalent salts: [Val or Ile‐GrA‐H+M]
+
, [Val or Ile‐GrA+MCl]
+
and [Val or Ile‐GrA+M]
2+
, where M is Ca or Mg or Zn. All these complexes have been subjected to collisionally activated dissociation (CAD). CAD of singly and doubly charged GrA and metal complexes exhibited the losses of water molecules, indicating the ligand preference of GrA. MS/MS and MS
3
of [Val or Ile‐GrA+MCl]
+
resulted in the elimination of chloride ion and water, respectively. The tandem mass spectrometry data of the complex [Val‐GrA+MCl]
+
suggest that chloride interaction is stronger in the presence of Ca than of Mg and Zn. This study reveals that GrA could interact with Ca, Mg, and Zn in metal ion form as well as in ion pair (MCl) form. The interactions of GrA with Ca support the proposal of a physical basis for the messenger role of Ca (Urry et al.,
J. Biol. Chem.
1982, 257: 6659–6661). Copyright © 2005 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/rcm.1947 |
| format | Article |
| fullrecord | <record><control><sourceid>crossref</sourceid><recordid>TN_cdi_crossref_primary_10_1002_rcm_1947</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1002_rcm_1947</sourcerecordid><originalsourceid>FETCH-LOGICAL-c727-46d822fe853d978fb3adc237af8c71901920af50af8aef3d8363ed218982a2863</originalsourceid><addsrcrecordid>eNo1kMtKAzEUhoMoWKvgI5ylINPm0mmSZRm8QcVNV26G00zSRuZGEpD6BO4EH9Encdrq4vDB-Q8_nI-Qa0YnjFI-DaaZMD2TJ2TEqJYZ5YKdkhHVOctmTKtzchHjG6WM5ZyOyNeiBez72htMvmuhc2Bra1LoYh9wB8POfxyjhG1lG2gwRoj94aaxKewgddCHbm0hbS34NtmA5r-sQOC3MH3eHPDa7jH0QFHDz-c3vPu0hU3Axhtf-RYWl-TMYR3t1R_HZHV_tyoes-XLw1OxWGZGcpnN5pXi3FmVi0pL5dYCK8OFRKeMZJoyzSm6fBiF1olKibmwFWdKK45czcWY3BxrzfBoDNaVffANhl3JaLn3WA4ey71H8QubD2aI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca 2+ /Mg 2+ /Zn 2+ and Cl − with gramicidin A</title><source>Access via Wiley Online Library</source><creator>Sudhir, Putty‐Reddy ; Wu, Hui‐Fen ; Zhou, Zi‐Cong</creator><creatorcontrib>Sudhir, Putty‐Reddy ; Wu, Hui‐Fen ; Zhou, Zi‐Cong</creatorcontrib><description>The peptide, gramicidin A (GrA), has been demonstrated to interact with divalent salts (CaCl
2
, MgCl
2
, and ZnCl
2
) using electrospray ionization mass spectrometry (ESI‐MS). The ESI‐MS analysis revealed different complexes formed due to the interaction of Val‐GrA and Ile‐GrA with divalent salts: [Val or Ile‐GrA‐H+M]
+
, [Val or Ile‐GrA+MCl]
+
and [Val or Ile‐GrA+M]
2+
, where M is Ca or Mg or Zn. All these complexes have been subjected to collisionally activated dissociation (CAD). CAD of singly and doubly charged GrA and metal complexes exhibited the losses of water molecules, indicating the ligand preference of GrA. MS/MS and MS
3
of [Val or Ile‐GrA+MCl]
+
resulted in the elimination of chloride ion and water, respectively. The tandem mass spectrometry data of the complex [Val‐GrA+MCl]
+
suggest that chloride interaction is stronger in the presence of Ca than of Mg and Zn. This study reveals that GrA could interact with Ca, Mg, and Zn in metal ion form as well as in ion pair (MCl) form. The interactions of GrA with Ca support the proposal of a physical basis for the messenger role of Ca (Urry et al.,
J. Biol. Chem.
1982, 257: 6659–6661). Copyright © 2005 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-4198</identifier><identifier>EISSN: 1097-0231</identifier><identifier>DOI: 10.1002/rcm.1947</identifier><language>eng</language><ispartof>Rapid communications in mass spectrometry, 2005-06, Vol.19 (11), p.1517-1521</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c727-46d822fe853d978fb3adc237af8c71901920af50af8aef3d8363ed218982a2863</citedby><cites>FETCH-LOGICAL-c727-46d822fe853d978fb3adc237af8c71901920af50af8aef3d8363ed218982a2863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sudhir, Putty‐Reddy</creatorcontrib><creatorcontrib>Wu, Hui‐Fen</creatorcontrib><creatorcontrib>Zhou, Zi‐Cong</creatorcontrib><title>An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca 2+ /Mg 2+ /Zn 2+ and Cl − with gramicidin A</title><title>Rapid communications in mass spectrometry</title><description>The peptide, gramicidin A (GrA), has been demonstrated to interact with divalent salts (CaCl
2
, MgCl
2
, and ZnCl
2
) using electrospray ionization mass spectrometry (ESI‐MS). The ESI‐MS analysis revealed different complexes formed due to the interaction of Val‐GrA and Ile‐GrA with divalent salts: [Val or Ile‐GrA‐H+M]
+
, [Val or Ile‐GrA+MCl]
+
and [Val or Ile‐GrA+M]
2+
, where M is Ca or Mg or Zn. All these complexes have been subjected to collisionally activated dissociation (CAD). CAD of singly and doubly charged GrA and metal complexes exhibited the losses of water molecules, indicating the ligand preference of GrA. MS/MS and MS
3
of [Val or Ile‐GrA+MCl]
+
resulted in the elimination of chloride ion and water, respectively. The tandem mass spectrometry data of the complex [Val‐GrA+MCl]
+
suggest that chloride interaction is stronger in the presence of Ca than of Mg and Zn. This study reveals that GrA could interact with Ca, Mg, and Zn in metal ion form as well as in ion pair (MCl) form. The interactions of GrA with Ca support the proposal of a physical basis for the messenger role of Ca (Urry et al.,
J. Biol. Chem.
1982, 257: 6659–6661). Copyright © 2005 John Wiley & Sons, Ltd.</description><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNo1kMtKAzEUhoMoWKvgI5ylINPm0mmSZRm8QcVNV26G00zSRuZGEpD6BO4EH9Encdrq4vDB-Q8_nI-Qa0YnjFI-DaaZMD2TJ2TEqJYZ5YKdkhHVOctmTKtzchHjG6WM5ZyOyNeiBez72htMvmuhc2Bra1LoYh9wB8POfxyjhG1lG2gwRoj94aaxKewgddCHbm0hbS34NtmA5r-sQOC3MH3eHPDa7jH0QFHDz-c3vPu0hU3Axhtf-RYWl-TMYR3t1R_HZHV_tyoes-XLw1OxWGZGcpnN5pXi3FmVi0pL5dYCK8OFRKeMZJoyzSm6fBiF1olKibmwFWdKK45czcWY3BxrzfBoDNaVffANhl3JaLn3WA4ey71H8QubD2aI</recordid><startdate>20050615</startdate><enddate>20050615</enddate><creator>Sudhir, Putty‐Reddy</creator><creator>Wu, Hui‐Fen</creator><creator>Zhou, Zi‐Cong</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20050615</creationdate><title>An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca 2+ /Mg 2+ /Zn 2+ and Cl − with gramicidin A</title><author>Sudhir, Putty‐Reddy ; Wu, Hui‐Fen ; Zhou, Zi‐Cong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c727-46d822fe853d978fb3adc237af8c71901920af50af8aef3d8363ed218982a2863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sudhir, Putty‐Reddy</creatorcontrib><creatorcontrib>Wu, Hui‐Fen</creatorcontrib><creatorcontrib>Zhou, Zi‐Cong</creatorcontrib><collection>CrossRef</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sudhir, Putty‐Reddy</au><au>Wu, Hui‐Fen</au><au>Zhou, Zi‐Cong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca 2+ /Mg 2+ /Zn 2+ and Cl − with gramicidin A</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><date>2005-06-15</date><risdate>2005</risdate><volume>19</volume><issue>11</issue><spage>1517</spage><epage>1521</epage><pages>1517-1521</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>The peptide, gramicidin A (GrA), has been demonstrated to interact with divalent salts (CaCl
2
, MgCl
2
, and ZnCl
2
) using electrospray ionization mass spectrometry (ESI‐MS). The ESI‐MS analysis revealed different complexes formed due to the interaction of Val‐GrA and Ile‐GrA with divalent salts: [Val or Ile‐GrA‐H+M]
+
, [Val or Ile‐GrA+MCl]
+
and [Val or Ile‐GrA+M]
2+
, where M is Ca or Mg or Zn. All these complexes have been subjected to collisionally activated dissociation (CAD). CAD of singly and doubly charged GrA and metal complexes exhibited the losses of water molecules, indicating the ligand preference of GrA. MS/MS and MS
3
of [Val or Ile‐GrA+MCl]
+
resulted in the elimination of chloride ion and water, respectively. The tandem mass spectrometry data of the complex [Val‐GrA+MCl]
+
suggest that chloride interaction is stronger in the presence of Ca than of Mg and Zn. This study reveals that GrA could interact with Ca, Mg, and Zn in metal ion form as well as in ion pair (MCl) form. The interactions of GrA with Ca support the proposal of a physical basis for the messenger role of Ca (Urry et al.,
J. Biol. Chem.
1982, 257: 6659–6661). Copyright © 2005 John Wiley & Sons, Ltd.</abstract><doi>10.1002/rcm.1947</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0951-4198 |
| ispartof | Rapid communications in mass spectrometry, 2005-06, Vol.19 (11), p.1517-1521 |
| issn | 0951-4198 1097-0231 |
| language | eng |
| recordid | cdi_crossref_primary_10_1002_rcm_1947 |
| source | Access via Wiley Online Library |
| title | An application of electrospray ionization tandem mass spectrometry to probe the interaction of Ca 2+ /Mg 2+ /Zn 2+ and Cl − with gramicidin A |
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